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Advisor(s)
Abstract(s)
This work reports the biological evaluation of nanosized hydroxyapatite (HAp) previously synthesized
by continuous-flow precipitation in a scaled-up meso oscillatory flow reactor (meso-OFR). Physicochemical
characterization of the synthesized HApsuggests high surface reactivity namely because of
its high specific surface area and low crystallinity. On the other hand, in vitro biomineralization assays
demonstrated the apatite-forming activity of the prepared HAp and their higher surface reactivity
when compared to a commercial HAp. Furthermore, human osteoblastic-like (Saos-2) cells culture
evidenced that the synthesized HAp stimulated cell proliferation, especially when applied at lower
concentrations (30 and 50 μg ml−1), although its cellular uptake behavior. Therefore, the prepared
HApshows immense potential as biomedical material, as well as drug and gene delivery vehicle. The
results are also very promising regarding further scaling up of the process, as the designed
methodology allow for the preparation in a continuous mode of nanosized HAp with controlled
physico-chemical properties.
Description
Keywords
Nanohydroxyapatite Calcium Phosphates Oscillatory Flow Reactor Mineralization SBF
Citation
CASTRO, Filipa; RIBEIRO, Viviana. P.; FERREIRA, António; OLIVEIRA, Ana L.; REIS, Rui L.; TEIXEIRA, José A.; ROCHA, Fernando - Continuous-flow precipitation as a route to prepare highly controlled nanohydroxyapatite: In vitro mineralization and biological evaluation. Materials Research Express. ISSN 2053-1591. Vol. 3, n.º 7 (2016), 13 p. (075404)
Publisher
IOP Publishing