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Modelling the inactivation of Bacillus subtilis spores by ethylene oxide processing

2009Artigo científico Acesso aberto
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dc.contributor.authorMendes, G. C.
dc.contributor.authorBrandão, Teresa R. S.
dc.contributor.authorSilva, C. L. M.
dc.date.accessioned2011-10-15T12:55:16Z
dc.date.available2011-10-15T12:55:16Z
dc.date.issued2009
dc.description.abstractEthylene oxide is currently a dominant agent in medical devices sterilization. This work intends to study the main effects and interactions of temperature (T), ethylene oxide (EO) concentration and relative humidity (RH) on commercial spore strips of Bacillus subtilis, var. niger (ATCC 9372) inactivation, the most common microorganism used in controlling the efficacy of the process. Experiments were carried out using a full factorial experimental design at two levels (23 factorial design). Limit target exposure conditions for EO concentration, temperature and relative humidity were 250 - 1000 mg EO/L, 40 - 60 °C and 50 - 90 %, respectively. Adopting a different approach from the first order kinetics, a Gompertz model was successfully applied in data fitting of the inactivation curves. Bacillus subtilis kinetic behaviour presented a sigmoidal inactivation with an initial shoulder (λ), followed by a maximum inactivation rate (kmax), these being model parameters. The influence of the referred environmental factors on lethality was assessed through λ and kmax estimates. It was concluded that, temperature and EO concentration were the most significant factors and consequently, additional experiments were carried out aiming at describing the parameters dependence on these process factors. Mathematical relations describing such dependences were successfully developed and included in the Gompertz kinetic model. The predictive ability of this integrated model was assessed, and its adequacy in predicting B. subtilis inactivation was proven.por
dc.identifier.citationMENDES, G. C. ; BRANDÃO, T. R. S. ; SILVA, C. L. M. - Modelling the inactivation of Bacillus subtilis spores by ethylene oxide processing. In World Congress of Sterilization, 10, Crete, Greece, 7-10 October, 2009 - Proceedings of the 10th World Congress of Sterilization. [S.l. : s.n., 2009]. ISBN 978-960-89379-5-6. 23 p.por
dc.identifier.urihttp://hdl.handle.net/10400.14/6213
dc.language.isoengpor
dc.peerreviewednopor
dc.titleModelling the inactivation of Bacillus subtilis spores by ethylene oxide processingpor
dc.typejournal article
dspace.entity.typePublication
person.familyNameBrandão
person.familyNameSilva
person.givenNameTeresa
person.givenNameCristina
person.identifierJ-9418-2012
person.identifier1473674
person.identifier.ciencia-id7B19-E23B-BB31
person.identifier.ciencia-id6611-634A-132C
person.identifier.orcid0000-0002-8857-6471
person.identifier.orcid0000-0002-0495-3955
person.identifier.ridJ-9418-2012
person.identifier.ridA-3568-2013
person.identifier.scopus-author-id6602174128
person.identifier.scopus-author-id7201387397
rcaap.rightsopenAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublicationebf7ead0-3772-4fd6-988c-f6b8722de84e
relation.isAuthorOfPublication4e2903de-0679-4cb1-981e-10de14c0c995
relation.isAuthorOfPublication.latestForDiscoveryebf7ead0-3772-4fd6-988c-f6b8722de84e

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