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Biopreservation strategies in combination with mild high pressure treatments in traditional Portuguese ready-to-eat meat sausage

2017Journal article Restricted access
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dc.contributor.authorCastro, S. M.
dc.contributor.authorKolomeytseva, M.
dc.contributor.authorCasquete, R.
dc.contributor.authorSilva, J.
dc.contributor.authorQueirós, R.
dc.contributor.authorSaraiva, J. A.
dc.contributor.authorTeixeira, P.
dc.date.accessioned2017-12-17T20:43:49Z
dc.date.available2017-12-17T20:43:49Z
dc.date.issued2017
dc.description.abstractThrombomodulin (TM) is an endothelial cell membrane protein that acts as a major cofactor in the protein C anticoagulant pathway. The EGF-like domains 4-6 of TM (TM456) are essential for PC activation. In this study, we proposed a liposomal recombinant TM conjugate to mimic the membrane TM structure and its anticoagulant activity. First, a DSPE-PEG2000-TM456 was successfully synthesized by site-specific conjugation of azido-TM456 with DSPE-PEG2000-DBCO via copper-free click chemistry quantitatively. Then, liposome-TM456 was fabricated via direct liposome formation with the DSPE-PEG2000-TM456 and other lipids. This liposomal formulation of TM456 retained protein C activation activity as that of TM456. Also, liposome-TM456 was much more stable and had a longer plasma half-life than TM456 and DSPE-PEG2000-TM456, respectively. Moreover, liposome-TM456 showed in vivo anticoagulant effect by decreasing the mortality from 80% to 20% in a thrombin-induced thromboembolism mouse model. The reported liposome-TM456 conjugate mimics the endothelial TM anticoagulation activity and may serve as an effective anticoagulant agent candidate for future development.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationCastro, S. M., Kolomeytseva, M., Casquete, R., Silva, J., Queiros, R., Saraiva, J. A., Teixeira, P. (2017). Biopreservation strategies in combination with mild high pressure treatments in traditional portuguese ready-to-eat meat sausage. Food Bioscience, 19, 65-72pt_PT
dc.identifier.doi10.1016/j.fbio.2017.05.008pt_PT
dc.identifier.eid85020926053
dc.identifier.eissn2212-4306
dc.identifier.issn2212-4292
dc.identifier.urihttp://hdl.handle.net/10400.14/23691
dc.identifier.wos000409155500009
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationNORTE-01–0145-FEDER-000030pt_PT
dc.subjectHigh pressurept_PT
dc.subjectBiopreservationpt_PT
dc.subjectListeria innocuapt_PT
dc.subjectPediococcus acidilacticipt_PT
dc.subjectBacteriocinpt_PT
dc.subjectMeat sausagept_PT
dc.titleBiopreservation strategies in combination with mild high pressure treatments in traditional Portuguese ready-to-eat meat sausagept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F71723%2F2010/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FMulti%2F50016%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FQUI%2F00062%2F2013/PT
oaire.citation.endPage72pt_PT
oaire.citation.startPage65pt_PT
oaire.citation.titleFood Biosciencept_PT
oaire.citation.volume19pt_PT
oaire.fundingStreamSFRH
oaire.fundingStream5876
oaire.fundingStream5876
person.familyNameSilva
person.familyNameQueirós
person.familyNameTeixeira
person.givenNameJoana
person.givenNameRui
person.givenNamePaula
person.identifier.ciencia-idB415-743D-D9F9
person.identifier.orcid0000-0003-3977-7418
person.identifier.orcid0000-0002-9112-5276
person.identifier.orcid0000-0002-6296-5137
person.identifier.ridM-1131-2014
person.identifier.ridJ-8678-2014
person.identifier.scopus-author-id7403023130
person.identifier.scopus-author-id55807131600
person.identifier.scopus-author-id7005895206
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication42069f79-2050-47f2-a42b-fab5c301e9a3
relation.isAuthorOfPublicationabd0be21-15fb-4656-b6e2-e59555eecea1
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relation.isAuthorOfPublication.latestForDiscoveryb918d4a2-b9f9-4c56-8318-111625e4d58b
relation.isProjectOfPublication42870125-9248-43dc-942f-cbaef23e56be
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