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Insights into the genome of Labrys portucalensis F11 and Rhodococcus sp. FP1, versatile xenobiotic-degrading bacteria isolated from an industrial contaminated soil

2017-10-18Conference object Restricted access
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dc.contributor.authorAmorim, Catarina L.
dc.contributor.authorMoreira, Irina S.
dc.contributor.authorHenriques, Isabel S.
dc.contributor.authorCastro, Paula M.L.
dc.date.accessioned2018-06-12T10:25:21Z
dc.date.available2018-06-12T10:25:21Z
dc.date.issued2017-10-18
dc.descriptionOral communicationpt_PT
dc.description.abstractBacteria play an important role in the cleanup of contaminated sites due to their ability to degrade an impressive variety of xenobiotic compounds, using them as carbon and energy source. Two xenobiotic-degrading bacteria, namely Labrys portucalensis F11 and Rhodococcus sp. FP1, were previously isolated from industrially contaminated sediments collected at Estarreja, northern Portugal, which is known for its large industrial chemical complex (from the 1950s). Both strains have versatile catabolic routes for the bioconversion of several xenobiotic compounds including pharmaceuticals (e.g. fluoroquinolones [1], fluoxetine [2], diclofenac and estradiols (ongoing work)) and industrial and agro-chemical compounds (e.g. fluorobenzene [3], fluorophenol [4], fluoroanilines [5] and bisphenols (ongoing work)). The whole-genome sequencing and assembly of the two xenobiotic-degrading bacteria and further functional annotation using the Rapid Annotation using Subsystems Technology (RAST) server was carried out. Assembled genomes are composed of 7,952,755 bp (F11) and 9,630,728 bp (FP1) with GC contents of 63.5 and 67.2%, respectively. Moreover, the draft genome sequence of strain F11 contains 7406 predicted coding DNA sequences (CDSs) that were classified into 505 different subsystems, whereas FP1 contains 9094 CDSs distributed by 454 subsystems. On both cases, the most abundant systems are those involved in the metabolism of amino acid derivatives and carbohydrates. In addition, both genomes harbor genes for the metabolism of aromatic compounds with about 3.2 and 4.5% of the total encoding genes of F11 and FP1 related to that feature. Gene clusters likely encoding different peripheral pathways for the aromatic compounds degradation such as benzoate, quinate, toluene, byphenyl and choloaromatics were found. Moreover, catechol 1,2-dioxygenases, catechol 2,3-dioxygenase and protocatechuate 3,4-dioxygenases encoding genes of the central pathway in the metabolism of various aromatic compounds were also present. In silico and experimental analysis revealed that F11 and FP1 had versatile catabolic metabolism of various aromatic xenobiotics, hence being good candidates to recycle xenobiotic compounds back into natural biogeochemical cycles. These new insights could help devising strategies for bioaugmentation of environments polluted with xenobiotics. Keywords: Whole-genome sequencing; xenobiotic-degrading bacteria; Labrys portucalensis F11; Rhodococcus sp. FP1.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationAmorim, C. L., Moreira, I. S., Henriques, I. S., Castro, P. M. L.. (2018). Insights into the genome of Labrys portucalensis F11 and Rhodococcus sp. FP1, versatile xenobiotic-degrading bacteria isolated from an industrial contaminated soil. BioMicroWorld 2017 - VII International Conference on Environmental, Industrial and applied Microbiology. 18-20 October, Madrid, Spain.pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.14/25047
dc.language.isoengpt_PT
dc.peerreviewednopt_PT
dc.relationIF/00492/2013pt_PT
dc.subjectWhole genome sequencingpt_PT
dc.subjectBioremediationpt_PT
dc.titleInsights into the genome of Labrys portucalensis F11 and Rhodococcus sp. FP1, versatile xenobiotic-degrading bacteria isolated from an industrial contaminated soilpt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F96481%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F87251%2F2012/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FMulti%2F50016%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FAMB%2F50017%2F2013/PT
oaire.citation.conferencePlaceMadrid, Spainpt_PT
oaire.citation.titleVII International Conference on Environmental, Industrial and Applied Microbiology - BioMicroWorld2017pt_PT
oaire.fundingStreamSFRH
oaire.fundingStreamSFRH
oaire.fundingStream5876
oaire.fundingStream5876
person.familyNameMoreira
person.familyNameCastro
person.givenNameIrina Susana Sousa
person.givenNamePaula
person.identifierK-5841-2014
person.identifier2013444
person.identifier.ciencia-id161E-A350-7FDE
person.identifier.ciencia-id7C1F-6C72-354A
person.identifier.orcid0000-0001-6516-0994
person.identifier.orcid0000-0001-8841-6606
person.identifier.ridK-5841-2014
person.identifier.ridM-8241-2013
person.identifier.scopus-author-id36798094100
person.identifier.scopus-author-id7102781782
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typeconferenceObjectpt_PT
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