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Platform design for extraction and isolation of Bromelain: complex formation and precipitation with carrageenan

2017Journal article Open access
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dc.contributor.authorCampos, Débora A.
dc.contributor.authorValetti, Nadia Woitovich
dc.contributor.authorOliveira, Ana
dc.contributor.authorPastrana-Castro, Lorenzo M.
dc.contributor.authorTeixeira, José A.
dc.contributor.authorPintado, Maria Manuela
dc.contributor.authorPicó, Guillermo
dc.date.accessioned2017-10-17T13:22:09Z
dc.date.available2017-10-17T13:22:09Z
dc.date.issued2017
dc.description.abstractThe main objective of this work was to investigate for the first time the molecular mechanism of com-plex formation between bromelain (a positively charged enzyme) and carrageenan (a natural strongpolyelectrolyte, negatively charged) using spectroscopy techniques and thermodynamic approaches. TheBromelain-Carrageenan complex showed a maximal non-solubility at pH around 5.1. The solubility wasdependent on pH and ionic strength of the medium. To re-dissolve the formed complex, the pH waschanged and 500 mM of NaCl was added to the initial solution, proving the columbic mechanism forthe formation of non-soluble complex. The formation of the carrageenan-bromelain complex increasedin 8◦C the enzyme thermal stability, while its biological activity was not modified. The amount of totalenzyme recovered in solution after precipitation with around 0.08% w/v of carrageenan was 85–90%.pt_PT
dc.description.versioninfo:eu-repo/semantics/acceptedVersionpt_PT
dc.identifier.citationCAMPOS, Débora A.; VALETTI, Nadia Woitovich; OLIVEIRA, Ana; PASTRANA-CASTRO, Lorenzo M.; TEIXEIRA, José A.; PINTADO, M. E.; PICO, Guillermo - Platform design for extraction and isolation of Bromelain: Complex formation and precipitation with carrageenan. Process Biochemistry. ISSN 1359-5113. Vol. 54 (2017), p. 156–161pt_PT
dc.identifier.doi10.1016/j.procbio.2016.12.014pt_PT
dc.identifier.eid85009251183
dc.identifier.eissn1873-3298
dc.identifier.issn1359-5113
dc.identifier.urihttp://hdl.handle.net/10400.14/23148
dc.identifier.wos000397832800021
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevier
dc.relationDevelopment and characterization of functional ingredients through valorization of pineapple by-products: production, bioactivity and potential application
dc.subjectProtein complexpt_PT
dc.subjectEnzyme mechanismpt_PT
dc.subjectCarrageenanpt_PT
dc.subjectPolyelectrolytespt_PT
dc.subjectBromelainpt_PT
dc.titlePlatform design for extraction and isolation of Bromelain: complex formation and precipitation with carrageenanpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleDevelopment and characterization of functional ingredients through valorization of pineapple by-products: production, bioactivity and potential application
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FMulti%2F50016%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/FARH/SFRH%2FBD%2F104074%2F2014/PT
oaire.citation.endPage161
oaire.citation.startPage156
oaire.citation.titleProcess Biochemistrypt_PT
oaire.citation.volume54
oaire.fundingStream5876
oaire.fundingStreamFARH
person.familyNameCampos
person.familyNameWoitovich Valetti
person.familyNameOliveira
person.familyNamePastrana
person.familyNameTeixeira
person.familyNamePintado
person.familyNamePicó
person.givenNameDébora
person.givenNameNadia
person.givenNameAna
person.givenNameLorenzo
person.givenNameJosé
person.givenNameMaria Manuela
person.givenNameGuillermo
person.identifierD-8675-2016
person.identifier1415634
person.identifier456608
person.identifier461789
person.identifier.ciencia-id1A16-FC5B-3468
person.identifier.ciencia-id4713-31C8-A356
person.identifier.ciencia-id2F13-AAE0-3405
person.identifier.orcid0000-0003-0174-6640
person.identifier.orcid0000-0001-7509-1160
person.identifier.orcid0000-0001-8012-4203
person.identifier.orcid0000-0002-6637-3462
person.identifier.orcid0000-0002-4918-3704
person.identifier.orcid0000-0002-0760-3184
person.identifier.orcid0000-0002-4764-9582
person.identifier.ridJ-3404-2013
person.identifier.ridA-6268-2012
person.identifier.ridF-5696-2013
person.identifier.scopus-author-id55250923400
person.identifier.scopus-author-id55937245800
person.identifier.scopus-author-id13402823200
person.identifier.scopus-author-id7004483898
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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