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Advisor(s)
Abstract(s)
The main objective of this work was to investigate for the first time the molecular mechanism of com-plex formation between bromelain (a positively charged enzyme) and carrageenan (a natural strongpolyelectrolyte, negatively charged) using spectroscopy techniques and thermodynamic approaches. TheBromelain-Carrageenan complex showed a maximal non-solubility at pH around 5.1. The solubility wasdependent on pH and ionic strength of the medium. To re-dissolve the formed complex, the pH waschanged and 500 mM of NaCl was added to the initial solution, proving the columbic mechanism forthe formation of non-soluble complex. The formation of the carrageenan-bromelain complex increasedin 8◦C the enzyme thermal stability, while its biological activity was not modified. The amount of totalenzyme recovered in solution after precipitation with around 0.08% w/v of carrageenan was 85–90%.
Description
Keywords
Protein complex Enzyme mechanism Carrageenan Polyelectrolytes Bromelain
Citation
CAMPOS, Débora A.; VALETTI, Nadia Woitovich; OLIVEIRA, Ana; PASTRANA-CASTRO, Lorenzo M.; TEIXEIRA, José A.; PINTADO, M. E.; PICO, Guillermo - Platform design for extraction and isolation of Bromelain: Complex formation and precipitation with carrageenan. Process Biochemistry. ISSN 1359-5113. Vol. 54 (2017), p. 156–161
Publisher
Elsevier