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A multi-omics analysis of the grapevine pathogen Lasiodiplodia theobromae reveals that temperature affects the expression of virulence- and pathogenicity-related genes

2019-12-01Journal article Open access
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dc.contributor.authorFélix, Carina
dc.contributor.authorMeneses, Rodrigo
dc.contributor.authorGonçalves, Micael F. M.
dc.contributor.authorTilleman, Laurentijn
dc.contributor.authorDuarte, Ana S.
dc.contributor.authorJorrín-Novo, Jesus V.
dc.contributor.authorPeer, Yves Van de
dc.contributor.authorDeforce, Dieter
dc.contributor.authorNieuwerburgh, Filip Van
dc.contributor.authorEsteves, Ana C.
dc.contributor.authorAlves, Artur
dc.date.accessioned2021-04-12T13:20:57Z
dc.date.available2021-04-12T13:20:57Z
dc.date.issued2019-12-01
dc.description.abstractLasiodiplodia theobromae (Botryosphaeriaceae, Ascomycota) is a plant pathogen and human opportunist whose pathogenicity is modulated by temperature. The molecular effects of temperature on L. theobromae are mostly unknown, so we used a multi-omics approach to understand how temperature affects the molecular mechanisms of pathogenicity. The genome of L. theobromae LA-SOL3 was sequenced (Illumina MiSeq) and annotated. Furthermore, the transcriptome (Illumina TruSeq) and proteome (Orbitrap LC-MS/MS) of LA-SOL3 grown at 25 °C and 37 °C were analysed. Proteins related to pathogenicity (plant cell wall degradation, toxin synthesis, mitogen-activated kinases pathway and proteins involved in the velvet complex) were more abundant when the fungus grew at 25 °C. At 37 °C, proteins related to pathogenicity were less abundant than at 25 °C, while proteins related to cell wall organisation were more abundant. On the other hand, virulence factors involved in human pathogenesis, such as the SSD1 virulence protein, were expressed only at 37 °C. Taken together, our results showed that this species presents a typical phytopathogenic molecular profile that is compatible with a hemibiotrophic lifestyle. We showed that L. theobromae is equipped with the pathogenesis toolbox that enables it to infect not only plants but also animals.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1038/s41598-019-49551-wpt_PT
dc.identifier.eid85072126103
dc.identifier.issn2045-2322
dc.identifier.pmcPMC6739476
dc.identifier.pmid31511626
dc.identifier.urihttp://hdl.handle.net/10400.14/32488
dc.identifier.wos000485216000014
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.titleA multi-omics analysis of the grapevine pathogen Lasiodiplodia theobromae reveals that temperature affects the expression of virulence- and pathogenicity-related genespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue1pt_PT
oaire.citation.titleScientific Reportspt_PT
oaire.citation.volume9pt_PT
person.familyNameFélix
person.familyNameGonçalves
person.familyNameTilleman
person.familyNameDuarte
person.familyNameJorrin Novo
person.familyNameEsteves
person.givenNameCarina
person.givenNameMicael
person.givenNameLaurentijn
person.givenNameAna Sofia
person.givenNameJesus V
person.givenNameAna Cristina
person.identifier295556
person.identifier82868
person.identifier.ciencia-id2015-2A43-2055
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person.identifier.orcid0000-0001-6849-6004
person.identifier.orcid0000-0002-8778-3604
person.identifier.orcid0000-0003-2239-2976
person.identifier.ridA-5212-2009
person.identifier.ridL-1656-2014
person.identifier.ridB-2939-2008
person.identifier.scopus-author-id57188979120
person.identifier.scopus-author-id56548119400
person.identifier.scopus-author-id56817825800
person.identifier.scopus-author-id57200010499
person.identifier.scopus-author-id56379803400
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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