Publication
Chemical characterization and antimicrobial activity of 6 plant essential oils against Pseudomonas syringae pv. actinidiae and P. syringae pv. actinidifoliorum
| dc.contributor.author | Silva, M. Nunes da | |
| dc.contributor.author | Machado, J. | |
| dc.contributor.author | Mazzaglia, A. | |
| dc.contributor.author | Balestra, G. M. | |
| dc.contributor.author | Vasconcelos, M. W. | |
| dc.contributor.author | Carvalho, S. M. P. | |
| dc.date.accessioned | 2024-11-06T11:53:16Z | |
| dc.date.available | 2024-11-06T11:53:16Z | |
| dc.date.issued | 2017 | |
| dc.description.abstract | Introduction: Kiwifruit bacterial canker (KBC), caused by Pseudomonas syringae pv. actinidiae (PSA), is currently the most destructive disease of kiwifruit worldwide. It was introduced in Portugal in 2010, leading to drastic production losses and plant mortality. Conversely, a closely related pathovar, P. syringae pv. actinidifoliorum (PSAF), only causes necrotic spots, not being associated with plant mortality. Current control techniques are expensive, consisting in the application of copper- and antibiotics-based compounds, with serious risks of environmental contamination and development of resistant bacteria. Therefore, the aim of this work was to characterize six plant essential oils against (anise, basil, cardamom, cumin, fennel and laurel) by gas chromatography–mass spectrometry (GC-MS) and evaluate their antimicrobial potential against different strains of PSA and PSAF. Methods: 1. Two highly virulent PSA strains (CFBP 7286 and 1F, isolated from Italy and France, respectively) and two less virulent PSAF strains (ICMP 18804 and ICMP 19441, isolated from New Zealand and Australia, respectively) were grown overnight in LB broth at 27 ᵒC and with shaking. 2. Bacterial inoculum (1-2x107 CFU.mL-1) was plated onto Nutrient Sucrose Agar medium and 20 μL droplets of each essential oil concentration were placed in 5 mm diameter paper discs (N = 3). 3. Plates were incubated at 28 ᵒC for 48 h, after which the inhibition zone was measured. 4. Essential oils chemical characterization was performed by GC-MS. Conclusions: Plant essential oils differed in their composition; however, compounds such as 3-carene, eucalyptol, camphor, fenchone and 4-terpineol were detected transversely in all oils. Although cumin presented the most simple composition, it showed MIC as low as 0.1 % for PSA 19441. In general, basil, cardamom and fennel had higher MICs, whereas anise had the lowest. PSAF strain 19441 was the most sensitive to almost all essential oils tested. | pt_PT |
| dc.description.version | info:eu-repo/semantics/publishedVersion | pt_PT |
| dc.identifier.uri | http://hdl.handle.net/10400.14/47134 | |
| dc.language.iso | eng | pt_PT |
| dc.peerreviewed | yes | pt_PT |
| dc.title | Chemical characterization and antimicrobial activity of 6 plant essential oils against Pseudomonas syringae pv. actinidiae and P. syringae pv. actinidifoliorum | pt_PT |
| dc.type | conference object | |
| dspace.entity.type | Publication | |
| oaire.citation.conferencePlace | Portugal | pt_PT |
| oaire.citation.title | IX International Symposium on Kiwifruit | pt_PT |
| rcaap.rights | openAccess | pt_PT |
| rcaap.type | conferenceObject | pt_PT |