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Protein quality assessment on saliva samples for biobanking purposes

2016-08-01Journal article Open access
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dc.contributor.authorRosa, Nuno
dc.contributor.authorMarques, Jéssica
dc.contributor.authorEsteves, Eduardo
dc.contributor.authorFernandes, Mónica
dc.contributor.authorMendes, Vera M.
dc.contributor.authorAfonso, Ângela
dc.contributor.authorDias, Sérgio
dc.contributor.authorPereira, Joaquim Polido
dc.contributor.authorManadas, Bruno
dc.contributor.authorCorreia, Maria José
dc.contributor.authorBarros, Marlene
dc.date.accessioned2021-05-28T10:53:00Z
dc.date.available2021-05-28T10:53:00Z
dc.date.issued2016-08-01
dc.description.abstractBiobank saliva sample quality depends on specific criteria applied to collection, processing, and storage. In spite of the growing interest in saliva as a diagnostic fluid, few biobanks currently store large collections of such samples. The development of a standard operating procedure (SOP) for saliva collection and quality control is fundamental for the establishment of a new saliva biobank, which stores samples to be made available to the saliva research community. Different collection methods were tested regarding total volume of protein obtained, protein content, and protein profiles, and the results were used to choose the best method for protein studies. Furthermore, the impact of the circadian variability and inter- and intraindividual differences, as well as the saliva sample stability at room temperature, were also evaluated. Considering our results, a sublingual cotton roll method for saliva collection proved to produce saliva with the best characteristics and should be applied in the morning, whenever possible. In addition, there is more variability in salivary proteins between individuals than in the same individual for a 5-month period. According to the electrophoretic protein profile, protein stability is guaranteed for 24 hours at room temperature and the protein degradation profile and protein identification were characterized. All this information was used to establish an SOP for saliva collection, processing, and storage in a biobank. We conclude that it is possible to collect saliva using an easy and inexpensive protocol, resulting in saliva samples for protein analysis with sufficient quality for biobanking purposes.pt_PT
dc.description.versioninfo:eu-repo/semantics/acceptedVersionpt_PT
dc.identifier.doi10.1089/bio.2015.0054pt_PT
dc.identifier.eid84981350223
dc.identifier.issn1947-5535
dc.identifier.pmid26937781
dc.identifier.urihttp://hdl.handle.net/10400.14/33365
dc.identifier.wos000382510300005
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.subjectBiobankpt_PT
dc.subjectBiobanking best practicespt_PT
dc.subjectProteomicspt_PT
dc.subjectQuality controlpt_PT
dc.subjectSample processingpt_PT
dc.subjectEvidence based biobankingpt_PT
dc.subjectPersonalized medicinept_PT
dc.subjectQuality managementpt_PT
dc.titleProtein quality assessment on saliva samples for biobanking purposespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage297pt_PT
oaire.citation.issue4pt_PT
oaire.citation.startPage289pt_PT
oaire.citation.titleBiopreservation and Biobankingpt_PT
oaire.citation.volume14pt_PT
person.familyNameRosa
person.familyNameEsteves
person.familyNameFernandes
person.familyNameDias
person.familyNameManadas
person.familyNameCorreia
person.familyNameBarros
person.givenNameNuno
person.givenNameEduardo
person.givenNameMónica
person.givenNameSergio
person.givenNameBruno
person.givenNameMaria
person.givenNameMarlene
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rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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