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Microbioma oral humano : análise microbiológica da diversidade de espécies de Candida na cavidade oral e em próteses removíveis
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Introdução: O microbioma oral é composto por um ecossistema fundamental para a manutenção da saúde oral. Entre os microrganismos que o agregam, o género Candida é um fungo que destaca se pela sua capacidade considerável de mudar do estado comensal para o patogénico, sobretudo em cenários favoráveis como má higienização, hábitos tabágicos, presença de diabetes e hipertensão arterial, tempo de uso da prótese, xerostomia e doenças orais. Objetivo: Este trabalho apresenta o objetivo de análisar a biodiversidade microbiológica das espécies do fungo Candida albicans em pacientes portadores das próteses removíveis. Foi aplicado um protocolo clínico de recolha de dados e amostras biológicas nos utentes da Clínica Dentária Universitária da Universidade Católica Portuguesa (CDU-UCP). Matérias e Métodos: Foram observados 69 pacientes todos portadores de próteses removíveis. Cada participante, forneceu amostras de saliva, biofilme dentário e do biofilme que se forma nas superfícies das próteses e da mucosa em contato com elas. O procedimento clínico incluiu a recolha de dados demográficos, como a idade, género dos pacientes, o tipo e material da prótese e o tempo de uso, hábitos de higiene orais e protéticas e condições sistémicas. Resultados: A maioria dos pacientes eram mulheres e as próteses mais frequentes as próteses acrílicas. Doenças sistémicas como diabetes mellitus e hipertensão arterial foram frequentes nos resultados. O período de uso ou hábito noturno de dormir com as próteses e a higiene oral deficitárias também foram fatores característicos na amostra estudada e, portanto, sugerem que é necessário aumentar a literacia no uso e manutenção das próteses dentárias. Considerações finais: Este trabalho mostrou ser possível fazer as recolhas e processamento das amostras para armazenamento em biobanco da FMD-UCP sendo agora necessário fazer a identificação molecular das várias espécies deste género.
Introduction:The oral microbiome is composed of an ecosystem that plays a fundamental role in maintaining oral health. Among the microorganisms that comprise it, the Candida genus stands out for its remarkable ability to shift from a commensal to a pathogenic state, particularly under favourable conditions such as poor hygiene, smoking habits, diabetes and hypertension, prosthesis wearing time, xerostomia, and oral diseases. Objective: This research aims to verify the microbiological biodiversity of Candida albicans species in patients wearing removable dentures. A clinical protocol was applied for data and biological sample collection from patients attending the University Dental Clinic of the Universidade Católica Portuguesa (CDU-UCP). Materials and Methods: A total of 69 patients, all users of removable dentures, were included. Each participant provided samples of saliva, dental biofilm, and the biofilm formed on the surfaces of the dentures and the mucosa in contact with them. The clinical procedure included the collection of demographic data such as age, gender, type and material of the prosthesis, duration of use, oral and prosthetic hygiene habits, and systemic conditions. Results: Most of the samples in this study were from female patients and most dentures were acrylic. Systemic diseases such as diabetes mellitus and hypertension were frequent findings in the analysis. The long period of use or the habit of sleeping with dentures and poor oral hygiene were also characteristic factors in the sample studied which can increase the risk of denture related stomatitis. Therefore, it is suggested that it is necessary to increase literacy in the use and maintenance of dentures. Final Considerations: This work showed that it is possible to collect and process the samples for storage in the biobank of FMD-UCP. It is now necessary to make the molecular identification of the various species of this genus.
Introduction:The oral microbiome is composed of an ecosystem that plays a fundamental role in maintaining oral health. Among the microorganisms that comprise it, the Candida genus stands out for its remarkable ability to shift from a commensal to a pathogenic state, particularly under favourable conditions such as poor hygiene, smoking habits, diabetes and hypertension, prosthesis wearing time, xerostomia, and oral diseases. Objective: This research aims to verify the microbiological biodiversity of Candida albicans species in patients wearing removable dentures. A clinical protocol was applied for data and biological sample collection from patients attending the University Dental Clinic of the Universidade Católica Portuguesa (CDU-UCP). Materials and Methods: A total of 69 patients, all users of removable dentures, were included. Each participant provided samples of saliva, dental biofilm, and the biofilm formed on the surfaces of the dentures and the mucosa in contact with them. The clinical procedure included the collection of demographic data such as age, gender, type and material of the prosthesis, duration of use, oral and prosthetic hygiene habits, and systemic conditions. Results: Most of the samples in this study were from female patients and most dentures were acrylic. Systemic diseases such as diabetes mellitus and hypertension were frequent findings in the analysis. The long period of use or the habit of sleeping with dentures and poor oral hygiene were also characteristic factors in the sample studied which can increase the risk of denture related stomatitis. Therefore, it is suggested that it is necessary to increase literacy in the use and maintenance of dentures. Final Considerations: This work showed that it is possible to collect and process the samples for storage in the biobank of FMD-UCP. It is now necessary to make the molecular identification of the various species of this genus.
Descrição
Palavras-chave
Microbioma Biofilme Saliva Próteses removíveis Microbiome Biofilm Dentures
Contexto Educativo
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Licença CC
Sem licença CC