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Abstract(s)
A saliva tem sido explorada como um fluido de diagnóstico precoce e para monitorização de patologias, tanto orais como sistémicas. No entanto, as estratégias de diagnóstico utilizadas continuam a ser baseadas na identificação de algumas moléculas, muitas vezes inespecíficas de determinada situação. Urge, pois, desenvolver estratégias que permitam avaliar o cenário molecular completo. O SalivaPrint é uma dessas estratégias. Contudo, para que esta abordagem possa ser utilizada no diagnóstico há que padronizar os perfis obtidos e identificar os fatores que afetam este perfil, tais como a idade, o género e a condição oral dos indivíduos. Nesse sentido, este trabalho tem como objetivos: 1. Verificar se há variação do SalivaPrint com a idade e com o género; 2. Verificar se há variação do SalivaPrint com a saúde oral; 3. Verificar se há relação dos parâmetros físico-químicos da saliva com os índices de saúde oral.
Foi recolhida saliva não estimulada de indivíduos do género feminino e masculino, saudáveis sistémicos e orais dos 7 aos 50, e um grupo dos 25-50 anos com patologia oral. Foi feita a determinação dos fatores físico-químicos, e dos perfis totais de proteínas por eletroforese capilar em micro-chips.
Verificou-se que existem alterações do SalivaPrint com a idade e com a saúde oral mas não com o género. A concentração total de proteína não difere, com o género, no entanto difere com a idade. O pH e o fluxo, não diferem, em indivíduos com patologia periodontal nem com a presença de cárie.
Uma vez que se observaram diferenças no SalivaPrint de pessoas saudáveis, é importante ter esse facto em conta na comparação com indivíduos doentes para isolar o efeito da doença dos efeitos de outros parâmetros.
Este tipo de trabalhos, é essencial para encontrar formas menos invasivas de diagnóstico que tenham em conta toda a variabilidade molecular e fisiológica do indivíduo.
Saliva is nowadays used to allow early diagnose and monitorization of both oral and systemic diseases. However, the diagnostic strategies used today are based on the identification of some molecules, often unspecific of a given condition. Therefore, it’s imperative to develop strategies that can assess the complete molecular scenario. SalivaPrint is one such strategies. However, for this approach to be used in the diagnosis, it is necessary to standardize the profiles obtained and to identify the factors that affect this profile, such as age, gender and the oral condition of the individuals. Therefore, this work aims to: 1. Verify if there’s any variation of SalivaPrint related to age or gender; 2. Verify if there’s any variation of SalivaPrint related to oral health; 3. Verify is there’s any relation between saliva physical and chemical parameters and the oral health indexes. Unstimulated whole saliva was collected from male and female individuals, systemic and orally healthy, aged between 7 and 50 and also in a group of oral disease bearers, aged between 25 and 50 years. Physical and chemical factors were determined, and total protein profile were established by using capillary electrophoresis in micro-chips. It was verified that there are changes in SalivaPrint related to age and oral health but not with gender. It was found that total protein concentration does not varies with gender however it varies with age. pH and flow do not vary either with periodontal disease neither the presence of tooth decay. Differences were observed in saliva protein profiles of healthy individuals. That is important to be considered when comparing to unhealthy individuals in order to isolate the disease effects from the effects of other parameters. This type of work it’s essential to find less invasive forms of diagnose that take into account all the molecular and physiological variability.
Saliva is nowadays used to allow early diagnose and monitorization of both oral and systemic diseases. However, the diagnostic strategies used today are based on the identification of some molecules, often unspecific of a given condition. Therefore, it’s imperative to develop strategies that can assess the complete molecular scenario. SalivaPrint is one such strategies. However, for this approach to be used in the diagnosis, it is necessary to standardize the profiles obtained and to identify the factors that affect this profile, such as age, gender and the oral condition of the individuals. Therefore, this work aims to: 1. Verify if there’s any variation of SalivaPrint related to age or gender; 2. Verify if there’s any variation of SalivaPrint related to oral health; 3. Verify is there’s any relation between saliva physical and chemical parameters and the oral health indexes. Unstimulated whole saliva was collected from male and female individuals, systemic and orally healthy, aged between 7 and 50 and also in a group of oral disease bearers, aged between 25 and 50 years. Physical and chemical factors were determined, and total protein profile were established by using capillary electrophoresis in micro-chips. It was verified that there are changes in SalivaPrint related to age and oral health but not with gender. It was found that total protein concentration does not varies with gender however it varies with age. pH and flow do not vary either with periodontal disease neither the presence of tooth decay. Differences were observed in saliva protein profiles of healthy individuals. That is important to be considered when comparing to unhealthy individuals in order to isolate the disease effects from the effects of other parameters. This type of work it’s essential to find less invasive forms of diagnose that take into account all the molecular and physiological variability.
Description
Keywords
Diagnóstico Saúde oral Proteínas Eletroforese Idade Género Diagnostics Oral health Proteins Electrophoresis Age Gender