Repository logo
 
Publication

Graphene oxide and graphene quantum dots as delivery systems of cationic porphyrins: photo-antiproliferative activity evaluation towards T24 human bladder cancer cells

2021-09-18Journal article Open access
Simple item page
dc.contributor.authorMenilli, Luca
dc.contributor.authorMonteiro, Ana R.
dc.contributor.authorLazzarotto, Silvia
dc.contributor.authorMorais, Filipe M. P.
dc.contributor.authorGomes, Ana T. P. C.
dc.contributor.authorMoura, Nuno M. M.
dc.contributor.authorFateixa, Sara
dc.contributor.authorFaustino, Maria A. F.
dc.contributor.authorNeves, Maria G. P. M. S.
dc.contributor.authorTrindade, Tito
dc.contributor.authorMiolo, Giorgia
dc.date.accessioned2021-10-07T13:37:16Z
dc.date.available2021-10-07T13:37:16Z
dc.date.issued2021-09-18
dc.description.abstractThe development of new photodynamic therapy (PDT) agents designed for bladder cancer (BC) treatments is of utmost importance to prevent its recurrence and progression towards more invasive forms. Here, three different porphyrinic photosensitizers (PS) (TMPyP, Zn-TMPyP, and P1-C5 ) were non-covalently loaded onto graphene oxide (GO) or graphene quantum dots (GQDs) in a one-step process. The cytotoxic effects of the free PS and of the corresponding hybrids were compared upon blue (BL) and red-light (RL) exposure on T24 human BC cells. In addition, intracellular reactive oxygen species (ROS) and singlet oxygen generation were measured. TMPyP and Zn-TMPyP showed higher efficiency under BL (IC50: 0.42 and 0.22 µm, respectively), while P1-C5 was more active under RL (IC50: 0.14 µm). In general, these PS could induce apoptotic cell death through lysosomes damage. The in vitro photosensitizing activity of the PS was not compromised after their immobilization onto graphene-based nanomaterials, with Zn-TMPyP@GQDs being the most promising hybrid system under RL (IC50: 0.37 µg/mL). Overall, our data confirm that GO and GQDs may represent valid platforms for PS delivery, without altering their performance for PDT on BC cells.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.3390/pharmaceutics13091512pt_PT
dc.identifier.eid85115391466
dc.identifier.issn1999-4923
dc.identifier.pmcPMC8470602
dc.identifier.pmid34575587
dc.identifier.urihttp://hdl.handle.net/10400.14/35419
dc.identifier.wos000699842200001
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectBladder cancerpt_PT
dc.subjectCationic porphyrinspt_PT
dc.subjectGraphene oxidept_PT
dc.subjectGraphene quantum dotspt_PT
dc.subjectNon-covalent hybridspt_PT
dc.subjectPhotodynamic therapypt_PT
dc.titleGraphene oxide and graphene quantum dots as delivery systems of cationic porphyrins: photo-antiproliferative activity evaluation towards T24 human bladder cancer cellspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue9pt_PT
oaire.citation.titlePharmaceuticspt_PT
oaire.citation.volume13pt_PT
person.familyNameMenilli
person.familyNameGomes
person.familyNameMoura
person.familyNameFateixa Caria
person.familyNameFaustino
person.familyNameTrindade
person.familyNameMIOLO
person.givenNameLuca
person.givenNameAna Teresa Peixoto de Campos
person.givenNameNuno M. M.
person.givenNameSara
person.givenNameMaria Amparo
person.givenNameTito
person.givenNameGIORGIA
person.identifier108827
person.identifier.ciencia-id6612-501E-6A79
person.identifier.ciencia-id0C1B-0EB6-4767
person.identifier.ciencia-id3511-B59D-C733
person.identifier.ciencia-idE915-34DA-4DAA
person.identifier.ciencia-idE417-E41B-4853
person.identifier.orcid0000-0003-2774-6411
person.identifier.orcid0000-0002-3201-0081
person.identifier.orcid0000-0002-9373-7006
person.identifier.orcid0000-0001-5958-466X
person.identifier.orcid0000-0003-4423-3802
person.identifier.orcid0000-0002-5456-7243
person.identifier.orcid0000-0003-1095-7124
person.identifier.ridR-1895-2017
person.identifier.ridC-7379-2013
person.identifier.ridJ-5787-2012
person.identifier.ridA-8030-2010
person.identifier.scopus-author-id57194868828
person.identifier.scopus-author-id55697408700
person.identifier.scopus-author-id14039621700
person.identifier.scopus-author-id33167546100
person.identifier.scopus-author-id6601979742
person.identifier.scopus-author-id7003743712
person.identifier.scopus-author-id6701910426
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication5aecf265-0171-4eee-a2b0-26f911d2b9eb
relation.isAuthorOfPublicationdc6dc93a-e504-4645-a70c-8f95f81589b5
relation.isAuthorOfPublication6d1e9777-c50b-46af-8491-9b31bb58d9b6
relation.isAuthorOfPublication5fe9e264-17cc-485c-b0cc-a3d15b6f3101
relation.isAuthorOfPublicationab29e601-494a-44fb-9bcf-6994e01e7476
relation.isAuthorOfPublicatione3bd6e0e-7370-4b3a-84ae-2a6d6aa67f36
relation.isAuthorOfPublication430a69be-45d7-4e7f-83ba-5e29b2c776db
relation.isAuthorOfPublication.latestForDiscovery6d1e9777-c50b-46af-8491-9b31bb58d9b6

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
38536516.pdf
Size:
7.75 MB
Format:
Adobe Portable Document Format
Download

Collections

CIIS - Contribuições em Revistas Científicas / Contribution to Journals