Browsing by Issue Date, starting with "2021-09-18"
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- Graphene oxide and graphene quantum dots as delivery systems of cationic porphyrins: photo-antiproliferative activity evaluation towards T24 human bladder cancer cellsPublication . Menilli, Luca; Monteiro, Ana R.; Lazzarotto, Silvia; Morais, Filipe M. P.; Gomes, Ana T. P. C.; Moura, Nuno M. M.; Fateixa, Sara; Faustino, Maria A. F.; Neves, Maria G. P. M. S.; Trindade, Tito; Miolo, GiorgiaThe development of new photodynamic therapy (PDT) agents designed for bladder cancer (BC) treatments is of utmost importance to prevent its recurrence and progression towards more invasive forms. Here, three different porphyrinic photosensitizers (PS) (TMPyP, Zn-TMPyP, and P1-C5 ) were non-covalently loaded onto graphene oxide (GO) or graphene quantum dots (GQDs) in a one-step process. The cytotoxic effects of the free PS and of the corresponding hybrids were compared upon blue (BL) and red-light (RL) exposure on T24 human BC cells. In addition, intracellular reactive oxygen species (ROS) and singlet oxygen generation were measured. TMPyP and Zn-TMPyP showed higher efficiency under BL (IC50: 0.42 and 0.22 µm, respectively), while P1-C5 was more active under RL (IC50: 0.14 µm). In general, these PS could induce apoptotic cell death through lysosomes damage. The in vitro photosensitizing activity of the PS was not compromised after their immobilization onto graphene-based nanomaterials, with Zn-TMPyP@GQDs being the most promising hybrid system under RL (IC50: 0.37 µg/mL). Overall, our data confirm that GO and GQDs may represent valid platforms for PS delivery, without altering their performance for PDT on BC cells.
- The use of interprofessional education programs in health care: a scoping review protocolPublication . Pina, Rui; Soares, Tânia; Rabiais, Isabel; Nunes, Elisabete; José, HelenaObjective: The objective of this scoping review will be to map and summarize the scientific evidence about interprofessional education programs (IPE) used in health care in order to inform future research. Introduction: The authors consider that implementation of an IPE program in their organizations will contribute to a better quality of health care. It must be sensitive to the evidence concerning the topic. This scoping review intends to map and summarize that evidence as it is very dispersed, making it difficult to approach. Inclusion criteria: Will be included studies and papers that address IPE programs used by health teams, with two or more professional groups, necessarily including nurses, in any health care setting. Scientific studies to health teams with students will be excluded, as well as papers in which the use of IPE program has not been evaluated. Methods: The proposed scoping review will be conducted in accordance with the JBI methodology for scoping reviews(1). Search strategy will aim to locate articles published in English, Portuguese and Spanish, both publish and unpublished primary studies, reviews and texts and opinion papers. Studies will be selected by two independent reviewers and any disagreement will be resolved through discussion or with a third reviewer. Data extraction will be performed by the same two independent reviewers using a data extraction tool developed by them. The data will be presented graphically or in diagrammatic or tabular form, accompanied by a narrative summary.
- Histomorphometric evaluation of bone-guided regeneration in maxillary sinus floor augmentation using nano-hydroxyapatite/beta-tricalcium phosphate composite biomaterial: a case reportPublication . Salviano, Saulo Henrique; Lopes, João Carlos Amorim; Brum, Igor da Silva; Frigo, Lúcio; Santos, Mário José dos; Consonni, Sílvio Roberto; Carvalho, Jorge José deBackground: The development of techniques in biomaterials design and production added to advanced surgical procedures which enabled better and more predictable clinical out-comes. Maxillary sinus floor augmentation (MSFA) is among the more studied bone-guided regeneration procedure in the literature. The MSFA could be considered the gold standard procedure for bone-guided regeneration as it provides suitable functional and aesthetic solutions to alveolar ridge atrophy due to tooth loss. Purpose: This study aimed to conduct a detailed histomorphometric evaluation of collagen production in SFAs bone-guided regeneration, using nano-hydroxyapatite/beta-tricalcium phos-phate (nano-HA/beta-TCP) composite. Patients and Methods: A 52-year-old female had the left upper second premolar con-demned due to periodontal disease, then a tooth implant replacement was planned. Due to maxillary sinus pneumatization, the MSFA had to be done before implant placement. Nano-HA/beta-TCP composite (2g) was used in the MSFA procedure. After nine months of the healing process, during the Cone Morse implant installation process, bone samples were collected for histologic analysis (sirius red, hematoxylin/eosin, polarized microscopy). Six months after implant installation, a ceramic crown was installed according to the patient's request. Results: Proper masticatory function and aesthetics were re-established. The histomorpho-metric evaluation indicated that nano-HA/beta-TCP composite did not show any area devoid of cellular activity in sirius red or hematoxylin/eosin staining and the percentage (%) of new bone collagen fibers was achieved using polarization technique evaluation. Conclusion: According to these results, nano-HA/beta-TCP composite presented clinical and histomorphometric properties suit to be used as bone-guided regeneration biomaterial in MSFA. Furthermore, nano-HA/beta-TCP composite provided a favorable nano-environment to bone cells, enhancing bone matrix production.