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Abstract(s)
Introdução: A periodontite é uma das doenças orais mais predominantes que afetam a maioria da população mundial. A periodontite grave, em particular, pode levar à incapacidade devido ao comprometimento da função mastigatória e estética ou edentulismo. Assim, as estratégias de intervenção que visem a sinalização Toll-like receptors (TLR) e as células T/B reguladoras do sistema imunitário podem ser uma abordagem promissora para reequilibrar a resposta imunitária e aliviar a perda óssea na doença periodontal. Objetivo: Avaliar alterações na expressão dos genes TLR na saliva de pacientes e validar o uso destas moléculas como biomarcadores no prognóstico e gestão dos casos clínicos de doença periodontal. Material e métodos: Foi feita a caracterização sociodemográfica e clínica de um grupo de pacientes com doença periodontal, bem como recolha de amostras de saliva e biofilme para posterior análise molecular. Foram selecionados pacientes de acordo com o grau/estadio da doença periodontal, sendo a expressão dos TLR avaliada por RT-PCR. Resultados: A população em estudo é constituída por 21 pacientes, entre os quais 52% eram mulheres e 48% homens, com prevalência da faixa etária dos 62-77 anos. Quanto ao diagnóstico, prevaleceram os pacientes com Periodontite, II, B. Foram identificadas alterações na expressão do gene TLR2 na saliva de um grupo representativo de amostras. A análise semiquantitativa demonstrou que a expressão relativa de TLR2 aumenta com a progressão da periodontite, embora o reduzido número de amostras não permita retirar conclusões definitivas. Conclusão: Sugere-se a continuidade deste estudo com um número mais elevado de amostras, bem como a identificação de novos marcadores moleculares que possam auxiliar no diagnóstico precoce e no plano de tratamento ou monitorização da doença periodontal.
Introduction: Periodontitis is one of the most prevalent oral diseases affecting the majority of the world's population. Severe periodontitis, in particular, can lead to disability due to impaired masticatory and esthetic function or edentulism. Thus, intervention strategies targeting Toll-like receptors (TLR) signaling and regulatory T/B cells of the immune system may be a promising approach to rebalance the immune response and alleviate bone loss in periodontal disease. Objective: To evaluate changes in the expression of TLR genes in the saliva of patients and validate the use of these molecules as biomarkers in the prognosis and management of clinical cases of periodontal disease. Material and methods: The sociodemographic and clinical characterization of a group of patients with periodontal disease was carried out, as well as the collection of saliva and biofilm samples for subsequent molecular analysis. Patients were selected according to the degree/stage of periodontal disease, and TLR expression was assessed by RT-PCR. Results: The study population consisted of 21 patients, among whom 52% were women and 48% men, with prevalence in the 62-77 age group. In terms of diagnosis, patients with Periodontitis, II, B prevailed. Alterations in the expression of the TLR2 gene were identified in the saliva of a representative group of samples. Semi-quantitative analysis showed that the relative expression of TLR2 increases with the progression of periodontitis, although the small number of samples does not allow definitive conclusions to be drawn. Conclusion: It is suggested that this study be continued with a higher number of samples, as well as the identification of new molecular markers that can help in the early diagnosis and treatment plan or monitoring of periodontal disease.
Introduction: Periodontitis is one of the most prevalent oral diseases affecting the majority of the world's population. Severe periodontitis, in particular, can lead to disability due to impaired masticatory and esthetic function or edentulism. Thus, intervention strategies targeting Toll-like receptors (TLR) signaling and regulatory T/B cells of the immune system may be a promising approach to rebalance the immune response and alleviate bone loss in periodontal disease. Objective: To evaluate changes in the expression of TLR genes in the saliva of patients and validate the use of these molecules as biomarkers in the prognosis and management of clinical cases of periodontal disease. Material and methods: The sociodemographic and clinical characterization of a group of patients with periodontal disease was carried out, as well as the collection of saliva and biofilm samples for subsequent molecular analysis. Patients were selected according to the degree/stage of periodontal disease, and TLR expression was assessed by RT-PCR. Results: The study population consisted of 21 patients, among whom 52% were women and 48% men, with prevalence in the 62-77 age group. In terms of diagnosis, patients with Periodontitis, II, B prevailed. Alterations in the expression of the TLR2 gene were identified in the saliva of a representative group of samples. Semi-quantitative analysis showed that the relative expression of TLR2 increases with the progression of periodontitis, although the small number of samples does not allow definitive conclusions to be drawn. Conclusion: It is suggested that this study be continued with a higher number of samples, as well as the identification of new molecular markers that can help in the early diagnosis and treatment plan or monitoring of periodontal disease.
Description
Keywords
Toll-like receptors Periodontite Biomarcadores moleculares Saliva Periodontitis Molecular biomarkers