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Objetivo: Determinar e interpretar do ponto de vista fisiopatológico o conjunto de interações entre as proteínas microbianas e do hospedeiro, na cavidade oral, no contexto da cárie dentária.
Enquadramento: A cavidade oral é um ecossistema complexo refletindo as interações moleculares estabelecidas entre proteínas do hospedeiro e proteínas produzidas pelo microbiota que o coloniza, tanto em estados de saúde como em diversas patologias.
É amplamente reconhecido o papel fulcral que o microbiota oral desempenha na etiologia da cárie dentária. Contudo, não se conhecem ainda a maioria dos intervenientes moleculares que medeiam esta ação. Para esclarecer este papel, urge identificar não só as moléculas envolvidas mas também os mecanismos moleculares em que elas participam.
As ferramentas bioinformáticas desempenham um papel importante nesta tarefa, fornecendo os meios que permitem estabelecer os pares de proteínas interatuantes, ou seja, o interactoma hospedeiro-microbiota em cárie dentária. Assim, o grupo de Biomedicina Computacional da UCP juntamente com o grupo de Bioinformática da UA e do Centro de Informática e Sistemas da Universidade de Coimbra desenvolveu a primeira ferramenta dedicada à cavidade oral, o Oralint que utiliza a informação molecular reunida no OralCard para gerar estes interactomas.
Metedologia: Identificar os microrganismos alterados na cárie dentária e fazer o levantamento das proteínas produzidas por eles. Atualizar a informação relativa às proteínas humanas alteradas na cárie dentária. As proteínas identificadas serão incluídas no OralCard e submetidas ao Oralint de modo a estabelecer o interactoma hospedeiro-microbiota na cárie dentária. Os resultados obtidos serão interpretados e discutidos à luz da fisiopatologia da cárie dentária. Expectativas: Atualizar o OralCard relativamente a proteínas humanas e microbianas relacionadas com cárie dentária. Estabelecer pela primeira vez o interactoma hospedeiro-microbiota oral na cárie dentária e, assim, contribuir para o esclarecimento dos mecanismos moleculares alterados no hospedeiro como consequência da ação dos microrganismos que colonizam a cavidade oral.
Objective: Determine and interpret the pathophysiological point of view, the set of interactions between microbial and host proteins in the oral cavity, in the context of dental caries. Framework: The oral cavity is a complex ecosystem that reflects the molecular interactions established between host proteins and proteins produced by microorganisms which colonize this system. This is true not only in health but also in several diseases. The pivotal role that oral microbiota plays in the etiology of dental caries is widely recognized. However, many of the molecular players that mediate this action are is still unknown. In order to clarify this role, we must not only identify the molecules involved but also the molecular mechanisms in which they participate. The bioinformatic tools play an important role in this task by providing the means for establish the pairs of interacting proteins, or the host- microbial interactome in tooth decay. In this sense, the group of Computational Biomedicine PCU in partnership with the Bioinformatics group of the AU and the Center for Informatics and Systems of University of Coimbra developed the first tool of its kind dedicated to the oral cavity , the Oralint using molecular information gathered in OralCard to generate these interactomas . Methodology: Identify organisms changed in tooth decay and mapping of the protein produced by them. Update the information on the altered proteins in human dental caries. The identified proteins are included in OralCard and submitted to Oralint to establish the microbiota - host interactome in tooth decay. The results will be interpreted and discussed in the light of the pathophysiology of dental caries. Expectations: Update the OralCard with information relating to human and microbial proteins related to dental caries. First establish the interactome oral host- microbiota in tooth decay and thus contribute to the elucidation of the molecular mechanisms altered in the host as a result of the action of microorganisms that colonize the oral cavity.
Objective: Determine and interpret the pathophysiological point of view, the set of interactions between microbial and host proteins in the oral cavity, in the context of dental caries. Framework: The oral cavity is a complex ecosystem that reflects the molecular interactions established between host proteins and proteins produced by microorganisms which colonize this system. This is true not only in health but also in several diseases. The pivotal role that oral microbiota plays in the etiology of dental caries is widely recognized. However, many of the molecular players that mediate this action are is still unknown. In order to clarify this role, we must not only identify the molecules involved but also the molecular mechanisms in which they participate. The bioinformatic tools play an important role in this task by providing the means for establish the pairs of interacting proteins, or the host- microbial interactome in tooth decay. In this sense, the group of Computational Biomedicine PCU in partnership with the Bioinformatics group of the AU and the Center for Informatics and Systems of University of Coimbra developed the first tool of its kind dedicated to the oral cavity , the Oralint using molecular information gathered in OralCard to generate these interactomas . Methodology: Identify organisms changed in tooth decay and mapping of the protein produced by them. Update the information on the altered proteins in human dental caries. The identified proteins are included in OralCard and submitted to Oralint to establish the microbiota - host interactome in tooth decay. The results will be interpreted and discussed in the light of the pathophysiology of dental caries. Expectations: Update the OralCard with information relating to human and microbial proteins related to dental caries. First establish the interactome oral host- microbiota in tooth decay and thus contribute to the elucidation of the molecular mechanisms altered in the host as a result of the action of microorganisms that colonize the oral cavity.
Descrição
Palavras-chave
Proteínas da cavidade oral OralOme Interações proteína-proteína Interatómica Cárie dentária Proteins of the oral cavity Protein-protein interactions Inter-atomic Dental caries