A carregar...
Publicação
Validação de um índice microbiano para avaliação do risco cárie em adultos
| Nome: | Descrição: | Tamanho: | Formato: | |
|---|---|---|---|---|
| 3.89 MB | Adobe PDF |
Resumo(s)
Introdução: A cárie dentária permanece uma das doenças orais mais prevalentes no mundo, apresentando etiologia multifatorial associada à interação entre fatores biológicos, comportamentais e ambientais. A integração de indicadores microbiológicos poderá complementar modelos convencionais de avaliação do risco, como o Caries Management by Risk Assessment (CAMBRA), permitindo uma caracterização mais abrangente do risco individual. Objetivos: Explorar o potencial da integração de indicadores microbiológicos na avaliação do risco de cárie em adultos, analisando a distribuição de espécies bacterianas cariogénicas e associadas à homeostasia da microbiota oral. Materiais e métodos: Realizou-se um estudo observacional transversal com 39 adultos da Clínica Dentária Universitária da Universidade Católica Portuguesa – Centro Regional de Viseu. Os participantes responderam a um questionário, foram submetidos a avaliação clínica intraoral e à recolha de saliva e biofilme oral. A análise microbiológica foi realizada por técnica molecular baseada no gene 16S rRNA, incidindo sobre Streptococcus mutans, Streptococcus sobrinus, Streptococcus mitis e Actinomyces johnsonii. Os resultados foram analisados de acordo com os grupos de severidade de cárie. Resultados: Streptococcus mutans foi detetada em todos os grupos, com maior prevalência nos indivíduos saudáveis. Streptococcus sobrinus apresentou distribuição irregular, enquanto Streptococcus mitis e Actinomyces johnsonii foram identificadas em todos os participantes. A presença isolada das espécies estudadas não permitiu discriminar os diferentes níveis de severidade de cárie. Conclusão: Os resultados sugerem que os indicadores microbiológicos, quando interpretados isoladamente, apresentam capacidade limitada para discriminar o risco de cárie. A reduzida dimensão da amostra, a distribuição desigual dos participantes e o desenho transversal limitaram a validação plena do índice microbiológico. Assim, os achados devem ser interpretados como exploratórios e como contributo preliminar para futuros estudos de validação.
Introduction: Dental caries remains one of the most prevalent oral diseases worldwide, with a multifactorial aetiology associated with the interaction of biological, behavioural, and environmental factors. The integration of microbiological indicators may complement conventional caries risk assessment models, such as Caries Management by Risk Assessment (CAMBRA), allowing a more comprehensive characterisation of individual risk. Objectives: To explore the potential of integrating microbiological indicators into caries risk assessment in adults by analysing the distribution of cariogenic bacterial species and species associated with oral microbiota homeostasis. Materials and Methods: A cross-sectional observational study was conducted with 39 adults attending the University Dental Clinic of the Universidade Católica Portuguesa – Viseu Regional Centre. Participants completed a questionnaire, underwent an intraoral clinical examination, and provided saliva and oral biofilm samples. Microbiological analysis was performed using a molecular technique based on the 16S rRNA gene, focusing on Streptococcus mutans, Streptococcus sobrinus, Streptococcus mitis, and Actinomyces johnsonii. The results were analysed according to caries severity groups. Results: Streptococcus mutans was detected in all groups, with higher prevalence among healthy individuals. Streptococcus sobrinus showed an irregular distribution, whereas Streptococcus mitis and Actinomyces johnsonii were identified in all participants. The isolated presence of the studied species did not allow discrimination between different levels of caries severity. Conclusion: The findings suggest that microbiological indicators, when interpreted individually, have limited ability to discriminate caries risk. The small sample size, unequal distribution of participants, and cross-sectional design limited the full validation of the microbiological index. Therefore, the findings should be interpreted as exploratory and as a preliminary contribution to future validation studies.
Introduction: Dental caries remains one of the most prevalent oral diseases worldwide, with a multifactorial aetiology associated with the interaction of biological, behavioural, and environmental factors. The integration of microbiological indicators may complement conventional caries risk assessment models, such as Caries Management by Risk Assessment (CAMBRA), allowing a more comprehensive characterisation of individual risk. Objectives: To explore the potential of integrating microbiological indicators into caries risk assessment in adults by analysing the distribution of cariogenic bacterial species and species associated with oral microbiota homeostasis. Materials and Methods: A cross-sectional observational study was conducted with 39 adults attending the University Dental Clinic of the Universidade Católica Portuguesa – Viseu Regional Centre. Participants completed a questionnaire, underwent an intraoral clinical examination, and provided saliva and oral biofilm samples. Microbiological analysis was performed using a molecular technique based on the 16S rRNA gene, focusing on Streptococcus mutans, Streptococcus sobrinus, Streptococcus mitis, and Actinomyces johnsonii. The results were analysed according to caries severity groups. Results: Streptococcus mutans was detected in all groups, with higher prevalence among healthy individuals. Streptococcus sobrinus showed an irregular distribution, whereas Streptococcus mitis and Actinomyces johnsonii were identified in all participants. The isolated presence of the studied species did not allow discrimination between different levels of caries severity. Conclusion: The findings suggest that microbiological indicators, when interpreted individually, have limited ability to discriminate caries risk. The small sample size, unequal distribution of participants, and cross-sectional design limited the full validation of the microbiological index. Therefore, the findings should be interpreted as exploratory and as a preliminary contribution to future validation studies.
Descrição
Palavras-chave
Cárie dentária Avaliação do risco de cárie Microbiota oral Indicadores microbiológicos CAMBRA 16S rRNA Dental caries Caries risk assessment Oral microbiota Microbiological indicators
Contexto Educativo
Citação
Editora
Licença CC
Sem licença CC