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Active packaging approach: impact of zinc oxide nanoparticles shape and size in antimicrobial activity
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Nanotechnology has been used in food packaging to improve material properties such as barrier to gases, thermal and light stability, and mechanical strength, offering active and intelligent functionalities that assure protection and preservation, together with biobased materials due to requirements for using less plastic packaging. Zinc oxide nanoparticles (ZnO NPs) has received a positive safety evaluation from European Food Safety Authority (EFSA) for packaging applications as transparent UV absorbers based on absence of significant migration in particulate form. It is also considered Generally Recognized As Safe (GRAS) by the FDA. ZnO NPs are also known to have good antimicrobial properties and therefore are suitable to be applied as active compounds. Despite the abundant literature addressing the use of ZnO NPs as antimicrobial component in packaging materials, the effect of particles size and morphology on the activity against different microorganisms is still poorly studied. In this work the impact of different shapes (spherical, sheet and flower) and sizes of nanoparticles was studied in their antimicrobial activity against Escherichia coli at 4 and 22 °C. ZnO NPs characterization was performed to assess their structural and physicochemical properties using scanning electron microscopy (SEM), X-ray powder diffraction (XRD), ultraviolet–visible spectroscopy (UV-VIS) and nitrogen adsorption–desorption for specific surface area determination. A further preliminary study was performed to characterize the antimicrobial activity of in situ ZnO NPs in bacterial nanocellulose (BNC) against E. coli at 4 and 22 °C.
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Mendes, A. R., Leite, A., Silva, F. A. G. S., & Granadeiro, C. et al. (2024). Active packaging approach: impact of zinc oxide nanoparticles shape and size in antimicrobial activity. 1-1. Poster session presented at Encontro Ciência 2024, Porto, Portugal.