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Abstract(s)
O objetivo deste trabalho foi desenvolver um dispositivo baseado em papel (μPAD) para a quantificação de ferro salivar uma vez que esta determinação pode constituir um potencial exame diagnóstico complementar para patologias como o cancro oral e a anemia. Para tal, desenvolveu-se um μPAD para quantificar ferro baseado na reação colorimétrica entre a batofenantrolina dissulfónica (BPS) e o catião ferro. O dispositivo possui um intervalo de aplicabilidade de 0,125 a 0,750 mg/L, com limites de deteção e quantificação de 0,034 mg/L e 0,091 mg/L, respetivamente. A estrutura final do μPAD apresenta duas camadas de papel de filtro Whatman 1 entre uma bolsa de plastificar. A camada superior possui um diâmetro de 9,5 mm, onde são adicionados 10 μL de solução reagente contendo BPS e hidroxilamina, e a camada inferior possui um diâmetro de 14 mm. Este dispositivo permanece estável quer a vácuo quer em contato com o ar durante pelo menos 1 mês. A digitalização do dispositivo deve ser feita entre 20 a 60 minutos após a adição da amostra. A exatidão da quantificação de ferro pelo dispositivo desenvolvido foi avaliada através da utilização de amostras de água certificadas, sendo ainda necessário otimizar a aplicação do método para amostras de saliva devido a presença de interferentes na matriz salivar.
Sendo assim, é possível concluir que o método desenvolvido permite quantificar de forma portátil, rápida e fácil o ferro em águas, o que o torna ideal para utilização em locais com recursos reduzidos, mas são necessários trabalhos futuros para otimizar a sua aplicação em amostras salivares.
The aim of this work was to develop a microfluidic paper-based analytical device (μPAD) for salivary iron quantification, as this determination may constitute a potential complementary diagnostic test for diseases such as oral cancer and anemia. For this purpose, based on the colorimetric reaction between bathophenanthroline disulfonic acid (BPS) and iron cation, a μPAD was developed to quantify iron. The device has an applicability range of 0.125 to 0.750 mg/L, with detection and quantification limits of 0.034 mg/L and 0.091 mg/L, respectively. The final structure of the μPAD consists of two layers of Whatman filter 1 between a plastic laminating pouch. The top layer, where 10 μL of reagent solution containing BPS and hydroxylamine are added, has a diameter of 9.5 mm and the bottom layer has a diameter of 14 mm. This device remains stable both under vacuum and in contact with air for at least 1 month. The device should be scanned 20 to 60 minutes after sample addition. The accuracy of the iron quantification by the developed device was evaluated using certified water samples. It’s still necessary to optimize the application of the method for saliva samples due to the presence of interfering salivary matrix. Thus, it can be concluded that the developed method allows portable, fast and easy quantification of iron in waters, which makes it ideal for use in low resource locations, but further work is needed to optimize its application in salivary samples.
The aim of this work was to develop a microfluidic paper-based analytical device (μPAD) for salivary iron quantification, as this determination may constitute a potential complementary diagnostic test for diseases such as oral cancer and anemia. For this purpose, based on the colorimetric reaction between bathophenanthroline disulfonic acid (BPS) and iron cation, a μPAD was developed to quantify iron. The device has an applicability range of 0.125 to 0.750 mg/L, with detection and quantification limits of 0.034 mg/L and 0.091 mg/L, respectively. The final structure of the μPAD consists of two layers of Whatman filter 1 between a plastic laminating pouch. The top layer, where 10 μL of reagent solution containing BPS and hydroxylamine are added, has a diameter of 9.5 mm and the bottom layer has a diameter of 14 mm. This device remains stable both under vacuum and in contact with air for at least 1 month. The device should be scanned 20 to 60 minutes after sample addition. The accuracy of the iron quantification by the developed device was evaluated using certified water samples. It’s still necessary to optimize the application of the method for saliva samples due to the presence of interfering salivary matrix. Thus, it can be concluded that the developed method allows portable, fast and easy quantification of iron in waters, which makes it ideal for use in low resource locations, but further work is needed to optimize its application in salivary samples.
Description
Keywords
μPAD Ferro Saliva Iron