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Extratos de fungos marinhos na desinfeção em endodontia : um modelo ex vivo
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Abstract(s)
A presente dissertação investiga o potencial de extratos de fungos marinhos, designadamente Penicillium lusitanum e Aspergillus affinis, como agentes antimicrobianos alternativos na descontaminação de canais radiculares em Endodontia. O estudo surge da necessidade de identificar substitutos biotecnológicos para o hipoclorito de sódio (NaClO), irrigante amplamente utilizado, mas cuja elevada citotoxicidade limita a sua segurança clínica. Foi utilizado um modelo ex vivo com dentes humanos monorradiculares contaminados com Enterococcus faecalis e Candida albicans. Os grupos experimentais tratados com os extratos de fungos marinhos foram comparados com NaClO 5,25% e solução salina estéril. Os resultados confirmaram a eficácia superior do NaClO, enquanto os extratos de fungos marinhos apresentaram atividade antimicrobiana limitada, não assegurando a eliminação total da contaminação. Em conclusão, embora os extratos estudados não superem o NaClO como irrigantes, podem constituir uma alternativa promissora como medicação intracanal, reforçando a relevância da bioprospecção marinha sustentável e da integração de terapias regenerativas baseadas em produtos naturais na prática endodôntica contemporânea.
This dissertation investigates the potential of marine fungal extracts, specifically Penicillium lusitanum and Aspergillus affinis, as alternative antimicrobial agents for root canal disinfection in endodontics. The study addresses the need for biotechnological substitutes to sodium hypochlorite (NaClO), a widely used irrigant whose high cytotoxicity limits clinical safety. An ex vivo model was employed using human single-rooted teeth contaminated with Enterococcus faecalis and Candida albicans. Experimental groups irrigated with marine extracts were compared to NaClO 5.25% and sterile saline controls. Results confirmed the superior antimicrobial efficacy of NaClO, while the fungal extracts exhibited limited activity, failing to completely eliminate microbial contamination under the tested conditions. In conclusion, although marine fungal extracts do not surpass NaClO as irrigants, they represent a promising intracanal medication, supporting sustainable marine bioprospecting and the integration of regenerative therapies based on natural products in contemporary endodontic practice.
This dissertation investigates the potential of marine fungal extracts, specifically Penicillium lusitanum and Aspergillus affinis, as alternative antimicrobial agents for root canal disinfection in endodontics. The study addresses the need for biotechnological substitutes to sodium hypochlorite (NaClO), a widely used irrigant whose high cytotoxicity limits clinical safety. An ex vivo model was employed using human single-rooted teeth contaminated with Enterococcus faecalis and Candida albicans. Experimental groups irrigated with marine extracts were compared to NaClO 5.25% and sterile saline controls. Results confirmed the superior antimicrobial efficacy of NaClO, while the fungal extracts exhibited limited activity, failing to completely eliminate microbial contamination under the tested conditions. In conclusion, although marine fungal extracts do not surpass NaClO as irrigants, they represent a promising intracanal medication, supporting sustainable marine bioprospecting and the integration of regenerative therapies based on natural products in contemporary endodontic practice.
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Keywords
Endodontia Irrigação Fungos marinhos Enterococcus faecalis Candida albicans Hipoclorito de sódio Endodontics Irrigation Marine fungi Sodium hypochlorite
Pedagogical Context
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Without CC licence