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Advisor(s)
Abstract(s)
In this work, an analytical flow system able to perform low pressure chromatography with amperometric
detection is presented. As case study, the determination of niacin (vitamin B3) in coffee brewed samples
was selected. The manifold comprised a 1.0 cm length monolithic column coated with didecyldimethylammonium
bromide, a laboratory-made boron doped diamond electrode, and featured in-line ionic
strength adjustment of the mobile phase. The figures of merit concerning the selected case study namely,
detection limit, 7.90 10 7 M, determination rate, ca. 10 samples h 1, mobile phase and ISA solution
consumption, ca. 2.6 mL per analysis, and CV, below 5% for retention time and peak height, showed
the competitiveness of this analytical strategy comparing to the described HPLC methods for niacin
determination. The strategy displays a simple configuration, low cost, fast and easy assembling,
foreseeing its use to general purpose applications.
Description
Keywords
Low pressure chromatography Flow analysis Coffee Vitamin B3 Niacin Boron doped diamond electrode Modified monolithic columns
Pedagogical Context
Citation
SANTOS, João Rodrigo; RANGEL, António O. S. S. - Development of a chromatographic low pressure flow injection system using amperometric detection: Application to the analysis of niacin in coffee. Food Chemistry. ISSN 0308-8146. N.º 187 (2015), p. 152–158
Publisher
Elsevier