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Listeria monocytogenes is a foodborne pathogen responsible for listeriosis, a serious illness with a high mortality rate typically ranging from 20-30%, but which can exceed 40% [1]. This condition primarily affects vulnerable populations such as pregnant women, newborns, the elderly, and those with weakened immune systems[2]. When contaminated food containing L. monocytogenes is ingested, the bacteria encounter various barriers within the human digestive system, including the gut microbiota, which significantly reduces its potential to cause an infection [3]. The objective of this work was to evaluate the interaction between L. monocytogenes and Escherichia coli, a representative human gut bacterium, by assessing their growth individually or in co-culture.