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Dual RNA sequencing of vitis vinifera during lasiodiplodia theobromae infection unveils host–pathogen interactions

2019-12-01Journal article Open access
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dc.contributor.authorGonçalves, Micael F. M.
dc.contributor.authorNunes, Rui B.
dc.contributor.authorTilleman, Laurentijn
dc.contributor.authorPeer, Yves Van De
dc.contributor.authorDeforce, Dieter
dc.contributor.authorNieuwerburgh, Filip Van
dc.contributor.authorEsteves, Ana C.
dc.contributor.authorAlves, Artur
dc.date.accessioned2021-04-12T14:42:41Z
dc.date.available2021-04-12T14:42:41Z
dc.date.issued2019-12-01
dc.description.abstractLasiodiplodia theobromae is one of the most aggressive agents of the grapevine trunk disease Botryosphaeria dieback. Through a dual RNA-sequencing approach, this study aimed to give a broader perspective on the infection strategy deployed by L. theobromae, while understanding grapevine response. Approximately 0.05% and 90% of the reads were mapped to the genomes of L. theobromae and Vitis vinifera, respectively. Over 2500 genes were significantly differentially expressed in infected plants after 10 dpi, many of which are involved in the inducible defense mechanisms of grapevines. Gene expression analysis showed changes in the fungal metabolism of phenolic compounds, carbohydrate metabolism, transmembrane transport, and toxin synthesis. These functions are related to the pathogenicity mechanisms involved in plant cell wall degradation and fungal defense against antimicrobial substances produced by the host. Genes encoding for the degradation of plant phenylpropanoid precursors were up-regulated, suggesting that the fungus could evade the host defense response using the phenylpropanoid pathway. The up-regulation of many distinct components of the phenylpropanoid pathway in plants supports this hypothesis. Moreover, genes related to phytoalexin biosynthesis, hormone metabolism, cell wall modification enzymes, and pathogenesis-related proteins seem to be involved in the host responses observed. This study provides additional insights into the molecular mechanisms of L. theobromae and V. vinifera interactions.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.3390/ijms20236083pt_PT
dc.identifier.eid85076167447
dc.identifier.issn1661-6596
dc.identifier.pmcPMC6928909
dc.identifier.pmid31816814
dc.identifier.urihttp://hdl.handle.net/10400.14/32497
dc.identifier.wos000504428300265
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectBotryosphaeria diebackpt_PT
dc.subjectDual RNA-Seqpt_PT
dc.subjectGrapevinept_PT
dc.subjectPathogenesispt_PT
dc.subjectPlant defensept_PT
dc.titleDual RNA sequencing of vitis vinifera during lasiodiplodia theobromae infection unveils host–pathogen interactionspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue23pt_PT
oaire.citation.titleInternational Journal of Molecular Sciencespt_PT
oaire.citation.volume20pt_PT
person.familyNameGonçalves
person.familyNameTilleman
person.familyNameEsteves
person.familyNameAlves
person.givenNameMicael
person.givenNameLaurentijn
person.givenNameAna Cristina
person.givenNameArtur
person.identifier82868
person.identifier84187
person.identifier.ciencia-id8610-4CD2-2881
person.identifier.ciencia-id0C11-7785-3C4E
person.identifier.ciencia-id2110-7954-9D7A
person.identifier.orcid0000-0003-2295-3374
person.identifier.orcid0000-0001-7584-1179
person.identifier.orcid0000-0003-2239-2976
person.identifier.orcid0000-0003-0117-2958
person.identifier.ridB-2939-2008
person.identifier.ridC-3645-2008
person.identifier.scopus-author-id56548119400
person.identifier.scopus-author-id56379803400
person.identifier.scopus-author-id7103319531
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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relation.isAuthorOfPublication0f9eb4ad-8ee4-407d-8a35-46591124d1db
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relation.isAuthorOfPublication.latestForDiscoverya8bf1cd0-54ff-49ca-876d-3c5058ed9466

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