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Nuclear magnetic resonance metabolomics of iron deficiency in soybean leaves

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dc.contributor.authorLima, Marta R. M.
dc.contributor.authorDiaz, Sílvia O.
dc.contributor.authorLamego, Inês
dc.contributor.authorGrusak, Michael A.
dc.contributor.authorVasconcelos, Marta
dc.contributor.authorGil, Ana M.
dc.date.accessioned2015-05-11T17:21:15Z
dc.date.available2015-05-11T17:21:15Z
dc.date.issued2014
dc.description.abstractIron (Fe) deficiency is an important agricultural concern that leads to lower yields and crop quality. A better understanding of the condition at the metabolome level could contribute to the design of strategies to ameliorate Fe-deficiency problems. Fe-sufficient and Fe-deficient soybean leaf extracts and whole leaves were analyzed by liquid 1H nuclear magnetic resonance (NMR) and high-resolution magic-angle spinning NMR spectroscopy, respectively. Overall, 30 compounds were measurable and identifiable (comprising amino and organic acids, fatty acids, carbohydrates, alcohols, polyphenols, and others), along with 22 additional spin systems (still unassigned). Thus, metabolite differences between treatment conditions could be evaluated for different compound families simultaneously. Statistically relevant metabolite changes upon Fe deficiency included higher levels of alanine, asparagine/aspartate, threonine, valine, GABA, acetate, choline, ethanolamine, hypoxanthine, trigonelline, and polyphenols and lower levels of citrate, malate, ethanol, methanol, chlorogenate, and 3-methyl-2-oxovalerate. The data indicate that the main metabolic impacts of Fe deficiency in soybean include enhanced tricarboxylic acid cycle activity, enhanced activation of oxidative stress protection mechanisms and enhanced amino acid accumulation. Metabolites showing accumulation differences in Fe-starved but visually asymptomatic leaves could serve as biomarkers for early detection of Fe-deficiency stress.por
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.citationLIMA, Marta R. M… [et al.] - Nuclear magnetic resonance metabolomics of iron deficiency in soybean leaves. Journal of Proteome research. ISSN 1535-3907. Vol. 13, n.º 6 (2014), p. 3075–3087por
dc.identifier.doi10.1021/pr500279f
dc.identifier.urihttp://hdl.handle.net/10400.14/17553
dc.identifier.wosWOS:000337074500032
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherAmerican Chemical Society
dc.relationEVALUATION OF THE METABOLIC RESPONSE OF OSTEOSARCOMA CELLS TO CONVENTIONAL AND NEW ANTICANCER DRUGS BY NUCLEAR MAGNETIC RESONANCE NMR SPECTROSCOPY AND MASS SPECTROMETRY MS METABONOMICS
dc.subjectChlorosispor
dc.subjectFe deficiencypor
dc.subjectGlycine max (soybean)por
dc.subjectNuclear magnetic resonance (NMR)por
dc.subjectHigh-resolution magic-angle spinning (HRMAS)por
dc.subjectMetabolomicspor
dc.subjectMultivariate analysispor
dc.titleNuclear magnetic resonance metabolomics of iron deficiency in soybean leavespor
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleEVALUATION OF THE METABOLIC RESPONSE OF OSTEOSARCOMA CELLS TO CONVENTIONAL AND NEW ANTICANCER DRUGS BY NUCLEAR MAGNETIC RESONANCE NMR SPECTROSCOPY AND MASS SPECTROMETRY MS METABONOMICS
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oaire.citation.endPage6
oaire.citation.endPage3087
oaire.citation.startPage13
oaire.citation.startPage3075
oaire.citation.titleJournal of Proteome Research
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person.familyNameLima
person.familyNameLamego
person.familyNameVasconcelos
person.familyNameGil
person.givenNameMarta
person.givenNameInês
person.givenNameMarta
person.givenNameAna
person.identifier74696
person.identifier.ciencia-id9815-79DC-CCEC
person.identifier.orcid0000-0002-1308-3541
person.identifier.orcid0000-0002-6079-1698
person.identifier.orcid0000-0002-5110-7006
person.identifier.orcid0000-0003-3766-4364
person.identifier.ridI-8166-2013
person.identifier.ridB-5440-2013
person.identifier.scopus-author-id44761160900
person.identifier.scopus-author-id14627797100
person.identifier.scopus-author-id55976900900
project.funder.identifierhttp://doi.org/10.13039/501100001871
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project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
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