Repository logo
 
Publication

Characterization of potential CLA-producing strains according to LA tolerance

2017Conference object Open access
Simple item page
dc.contributor.authorFontes, Ana Luiza
dc.contributor.authorPimentel, Lígia
dc.contributor.authorSalsinha, Ana Sofia
dc.contributor.authorCardoso, Beatriz
dc.contributor.authorAndrade, José Carlos
dc.contributor.authorRodriguéz-Alcalá, Luís Miguel
dc.contributor.authorGomes, Ana Maria
dc.date.accessioned2018-11-26T18:23:57Z
dc.date.available2018-11-26T18:23:57Z
dc.date.issued2017
dc.description.abstractBackground: Conjugated linoleic acid (CLA) isomers are naturally produced from dietary linoleic acid (LA) by ruminal bacteria. However, strains of lactobacilli, bifidobacteria and propionibacteria have also demonstrated the ability to produce those bioactive fatty acids. In vitro studies normally test CLA production at 0.5 mg/mL of LA, but possibly some strains can tolerate higher concentrations and if they are producers, CLA yields may probably be higher. This work aims to determine the maximum LA concentration that potential CLA-producing strains can tolerate in an in vitro production assay. Method: Thirty five lactobacilli, 17 bifidobacteria, 1 propionibacterium and 1 lactococcus strains were submitted to a modified method described by Roméro-Pérez et al. [1]. Activated strains were inoculated in MRS agar plates containing 1, 2 or 5 mg/mL of LA for 48 h at 37 ºC. Strains from the agar plate at the highest LA tolerated concentration were then spiked into MRS broth with LA in a 96-well microplate and subsequently incubated at 37 ºC for 48 h; growth curves were recorded at 600, 620 and 660 nm in a microplate reader. Cultures without substrate were used as control. Agar plates experiment was carried out in duplicate and microplate experiment in triplicate. Results & Conclusions: Among the strains tested, 18 (~33%) could not grow at the lowest LA concentration assayed, having considered their tolerance below 1 mg/mL. A group of 16 strains showed growth at 1 mg/mL of LA, 8 tolerated up to 2 mg/mL and 12 up to 5 mg/mL. At 5 mg/mL lactobacilli was the dominant group, whereas at 2 mg/mL was bifidobacteria. At <1 mg/mL and 1 mg/mL the distribution of lactobacilli and bifidobacteria was similar. The propionibacterium and the lactococcus strains could grow a 1 mg/mL. In conclusion, potential CLA-producing strains exhibit different LA tolerance degrees. This is a parameter to consider in future production tests.
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationFontes, A.L.; Pimentel, L.; Salsinha, A.S.; Cardoso, B.; Andrade, J.C.; Rodriguez-Alcalá, L.M.; Gomes, A.M. (2017) Characterization of potential CLA-producing strains according to LA tolerance. MICROBIOTEC ´17 - 2017, Book of Abstracts - Congress of Microbiology and Biotechnology, 7-9 December, Porto, Portugal.pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.14/26126
dc.language.isoengpt_PT
dc.relation.publisherversionhttp://www.porto.ucp.pt/sites/default/files/files/Biotecnologia/Microbiotec17/BookofAbstracts_15_12_2017.pdfpt_PT
dc.subjectLA tolerance
dc.subjectProbiotics
dc.subjectCLA
dc.subjectLactic acid bacteria
dc.subjectBifidobacterium
dc.titleCharacterization of potential CLA-producing strains according to LA tolerancept_PT
dc.typeconference object
dspace.entity.typePublication
oaire.citation.titleMICROBIOTEC ´17 - 2017pt_PT
person.familyNamePimentel
person.familyNameAndrade
person.familyNameGomes
person.givenNameLígia
person.givenNameJosé
person.givenNameAna Maria
person.identifier534450
person.identifier684026
person.identifier162628
person.identifier.ciencia-id6618-9DD9-3C65
person.identifier.ciencia-id2D1D-00CA-016F
person.identifier.ciencia-idAA12-BFC1-B2E3
person.identifier.orcid0000-0002-6656-017X
person.identifier.orcid0000-0002-2403-6062
person.identifier.orcid0000-0001-7883-2446
person.identifier.ridK-3254-2014
person.identifier.ridB-9944-2013
person.identifier.scopus-author-id22035944100
person.identifier.scopus-author-id55415967800
person.identifier.scopus-author-id57190668768
rcaap.rightsopenAccesspt_PT
rcaap.typeconferenceObjectpt_PT
relation.isAuthorOfPublicationd6520be9-873a-41f9-9aa1-96a5b65e8070
relation.isAuthorOfPublicationb5156d42-c49f-4991-8aa9-fc77cfff8a33
relation.isAuthorOfPublicationc18c2fb1-f3dc-4274-a646-23d456432798
relation.isAuthorOfPublication.latestForDiscoveryc18c2fb1-f3dc-4274-a646-23d456432798

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
41793422.pdf
Size:
214.68 KB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
3.44 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

CBQF - Documentos de Conferências / Conference Objects