| Name: | Description: | Size: | Format: | |
|---|---|---|---|---|
| 1.18 MB | Adobe PDF |
Advisor(s)
Abstract(s)
tHigh relative air humidity (RH ≥ 85%) during growth disturbs stomatal functioning, resulting in excessivewater loss in conditions of high evaporative demand. We investigated the expression of nine abscisic acid(ABA)-related genes (involved in ABA biosynthesis, oxidation and conjugation) and two non-ABA relatedgenes (involved in the water stress response) aiming to better understand the mechanisms underlyingcontrasting stomatal functioning in plants grown at high RH. Four rose genotypes with contrasting sen-sitivity to high RH (one sensitive, one tolerant and two intermediate) were grown at moderate (62 ± 3%)or high (89 ± 4%) RH. The sensitive genotype grown at high RH showed a significantly higher stomatalconductance (gs) and water loss in response to closing stimuli as compared to the other genotypes. More-over, high RH reduced the leaf ABA concentration and its metabolites to a greater extent in the sensitivegenotype as compared to the tolerant one. The large majority of the studied genes had a relevant role onstomatal functioning (NCED1, UGT75B2, BG2, OST1, ABF3 and Rh-APX) while two others showed a minorcontribution (CYP707A3 and BG1) and AAO3, CYP707A1 and DREB1B did not contribute to the tolerancetrait. These results show that multiple genes form a highly complex regulatory network acting togethertowards the genotypic tolerance to high RH.
Description
Keywords
Abscisic acid Stomatal anatomy Tetraploid roses Vapor pressure deficit Water loss
Citation
CARVALHO, Dália R.A.; VASCONCELOS, Marta W.; LEE, Sangseok; KONING-BOUCOIRAN, Carole F.S.; VREUGDENHIL, Dick; KRENS, Frans A.; HEUVELINK, Ep; CARVALHO, Susana M. P. - Gene expression and physiological responses associated to stomatal functioning in Rosa x hybrida grown at high relative air humidity. Plant Science. ISSN 0168-9452. Vol. 253 (2016), p. 154-163
Publisher
Elsevier