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Advisor(s)
Abstract(s)
tHigh relative air humidity (RH ≥ 85%) during growth disturbs stomatal functioning, resulting in excessivewater loss in conditions of high evaporative demand. We investigated the expression of nine abscisic acid(ABA)-related genes (involved in ABA biosynthesis, oxidation and conjugation) and two non-ABA relatedgenes (involved in the water stress response) aiming to better understand the mechanisms underlyingcontrasting stomatal functioning in plants grown at high RH. Four rose genotypes with contrasting sen-sitivity to high RH (one sensitive, one tolerant and two intermediate) were grown at moderate (62 ± 3%)or high (89 ± 4%) RH. The sensitive genotype grown at high RH showed a significantly higher stomatalconductance (gs) and water loss in response to closing stimuli as compared to the other genotypes. More-over, high RH reduced the leaf ABA concentration and its metabolites to a greater extent in the sensitivegenotype as compared to the tolerant one. The large majority of the studied genes had a relevant role onstomatal functioning (NCED1, UGT75B2, BG2, OST1, ABF3 and Rh-APX) while two others showed a minorcontribution (CYP707A3 and BG1) and AAO3, CYP707A1 and DREB1B did not contribute to the tolerancetrait. These results show that multiple genes form a highly complex regulatory network acting togethertowards the genotypic tolerance to high RH.
Description
Keywords
Abscisic acid Stomatal anatomy Tetraploid roses Vapor pressure deficit Water loss
Pedagogical Context
Citation
CARVALHO, Dália R.A.; VASCONCELOS, Marta W.; LEE, Sangseok; KONING-BOUCOIRAN, Carole F.S.; VREUGDENHIL, Dick; KRENS, Frans A.; HEUVELINK, Ep; CARVALHO, Susana M. P. - Gene expression and physiological responses associated to stomatal functioning in Rosa x hybrida grown at high relative air humidity. Plant Science. ISSN 0168-9452. Vol. 253 (2016), p. 154-163
Publisher
Elsevier