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- Antiulcerative and antitumoral properties of spent brewer's yeast peptide extracts for incorporation in foodsPublication . Amorim, Manuela; Pereira, Joana; Monteiro, Karin; Ruiz, Ana; Pinheiro, Hélder; Carvalho, João; Pintado, ManuelaToday, the inflammatory bowel diseases such as ulcerative colitis are increasing and have been associated to contemporary people. Additionally, the increasing of cancer in the world is also a current fact. These issues are attracting attention in the search of new ingredients used in the prevention and improvement of these diseases. Particularly, several compounds, when added to foods, are able to positively stimulate certain biological systems leading to health improvement. Thereby, extracts obtained from the combined autolysis and hydrolysis of yeast cells possess compounds such peptides and amino acids, which eventually may control the production of inflammatory cytokines, thus leading to an anti-inflammatory activity and antitumoral properties. Thus, the main objective of the present work is to study the antiulcerative and antitumoral potential of yeast peptide extract for further incorporation in functional foods to prevent or improve symptoms in these chronic diseases. Peptide concentrates obtained by hydrolysis of spent brewer yeast proteins with extracts of Cynara cardunculus were studied using animal models to prove possible protection of the stomach mucosa against ulcerative lesions caused by oral administration of absolute ethanol. The peptide fraction below 3 kDa (peptidic extract) from yeast proteins was able to reduce gastric injuries to significant levels (p < 0.05). Additionally, the anti-proliferative activity of these extracts in nine cell lines of different human tumor was tested. The results exhibited a promising antiproliferative activity against leukemia cells. These results suggest that this new peptide extract can be used to develop new functional foods, although further studies are required.
- Antiulcerogenic activity of peptide concentrates obtained from hydrolysis of whey proteins by proteases from Cynara cardunculusPublication . Tavares, T.G.; Monteiro, K.M.; Possenti, A.; Pintado, M. E.; Carvalho, J. E.; Malcata, F. XavierPeptide concentrates generated by hydrolysis of whey with aqueous extracts of flowers of Cynara cardunculus were studied for possible protection of the stomach mucosa against ulcerative lesions caused by oral administration of absolute ethanol. Both the whole peptide fraction obtained via hydrolysis of whey protein concentrate (peptide concentrate, PepC) and its fraction below 3 kDa (PepCF) were able to reduce gastric injuries to significant levels (p < 0.05). Single-dose experiments, using 100 mg kg(-1) body weight (bw) of either PepCF or PepC, led to 68.5% and 37.4% protection, respectively - which compare well with 93.4% protection by 200 mg kg (1) bw carbenoxolone (a positive control). No dose-response correlation could be demonstrated. Gastric cytoprotection by PepCF appears to depend on sulphydryl-containing moieties, whereas PepC likely protects the gastric mucosa via the prostaglandin cycle and production of nitric oxide.