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- The contribution of an intergenerational program to build bridges between school and community and to promote old people’s well-being and mutual learningPublication . Azevedo, Cláudia; Palmeirão, CristinaThe intergenerational program presented here is a part of a major research, which includes several projects developed in the north of Portugal. The challenges of aging societies and the need to create strong and effective bonds of solidarity between generations lead us to develop an intergenerational program between a school (students (N=25 from seventh grade) and a nursing home (N=15). We wish to register on Lifelong Education issue, for the reason that school is the main field where the changes of attitudes can be reality, namely myths and stereotypes to old people. The aims of this study were: i) encourage the educational community to the practice a new (way) of citizenship; ii) Develop relationships between a group of students from the Matosinhos’ school grouping44, and old people living in the Sant’Ana’s nursing home45 enhancing thus the intergenerational networks and iii) Promote an active aging by sharing and valuing the generational knowledge. The encounters, twice a week, on Civic Education class embraced an encouragement to mutual learning based on the differents subjects. The elders refer the important of maintain contact and dialogue with the youth and the opportunity of learning. The students refer the occasion of teaching differents subjects to the elders and stimulation of solidarity bonds and great harmony. Teachers said that the greatest impact was in writing, with the development of various types of texts, in pairs or in groups. Seniors and juniors did their best so that their meetings were fruitful and fulfilled its main goal: mutual learning.
- The chemopreventive effect of the dietary compound kaempferol on the MCF-7 human breast cancer cell line is dependent on inhibition of glucose cellular uptakePublication . Azevedo, Cláudia; Correia-Branco, Ana; Araújo, João R.; Guimarães, João T.; Keating, Elisa; Martel, FátimaOur aim was to investigate the effect of several dietary polyphenols on glucose uptake by breast cancer cells. Uptake of H-3-deoxy-D-glucose (H-3-DG) by MCF-7 cells was time-dependent, saturable, and inhibited by cytochalasin B plus phloridzin. In the short-term (26min), myricetin, chrysin, genistein, resveratrol, kaempferol, and xanthohumol (10-100 mu M) inhibited H-3-DG uptake. Kaempferol was found to be the most potent inhibitor of H-3-DG uptake [IC50 of 4 mu M (1.6-9.8)], behaving as a mixed-type inhibitor. In the long-term (24h), kaempferol (30 mu M) was also able to inhibit H-3-DG uptake, associated with a 40% decrease in GLUT1 mRNA levels. Interestingly enough, kaempferol (100 mu M) revealed antiproliferative (sulforhodamine B and H-3-thymidine incorporation assays) and cytotoxic (extracellular lactate dehydrogenase activity determination) properties, which were mimicked by low extracellular (1mM) glucose conditions and reversed by high extracellular (20mM) glucose conditions. Finally, exposure of cells to kaempferol (30 mu M) induced an increase in extracellular lactate levels over time (to 731 +/- 32% of control after a 24 h exposure), due to inhibition of MCT1-mediated lactate cellular uptake. In conclusion, kaempferol potently inhibits glucose uptake by MCF-7 cells, apparently by decreasing GLUT1-mediated glucose uptake. The antiproliferative and cytotoxic effect of kaempferol in these cells appears to be dependent on this effect.