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- From phyto to agromining: past, present, and future scopePublication . Novo, Luís A. B.; Castro, Paula M. L.; Alvarenga, Paula; Silva, Eduardo Ferreira daThe accumulation of valuable metals in plants has fascinated scientists ever since the 1700 s (Chaptal 1790; Malte-Brun 1824).
- Recent advances in phytoremediation of soil contaminated by industrial waste: a road map to a safer environmentPublication . Bernardino, Cassiano A. R.; Mahler, Claudio F.; Alvarenga, Paula; Castro, Paula M. L.; Silva, Eduardo Ferreira da; Novo, Luís A. B.The advent of the industrial revolution has boosted human and technological development, but at a price. Contaminants have been increasingly accumulating across wider areas scattered around the world, making pollution a major planetary issue. Among these pollutants, those related to industrial waste are perhaps the most threatening due to their broad variety and capacity to migrate through the air, water, and soil, enter the food chain, and cause a deleterious impact on life and the environment. Finding solutions to deter industrial pollution has thus become the need of the hour. However, conventional techniques have often proven unworkable due to their cost and harmful collateral effects to the environment. In this light, a plant-based technique with the capacity to stabilize, extract, and/or degrade pollutants known as phytoremediation, has emerged as a promising alternative due to its inexpensiveness and environment-friendly character. Here, we review the latest advances on phytoremediation of sites afflicted by industrial pollution and outline the future scope of this green technique.
- Genome editing weds CRISPR: what is in it for phytoremediation?Publication . Basharat, Zarrin; Novo, Luís A. B.; Yasmin, AzraThe arrival of sequence-specific endonucleases that allow genome editing has shaken the pillars of basic and applied plant biology. Clustered regularly interspaced palindromic repeats (CRISPR) is a revolutionary genome-engineering tool that enables the enhancement of targeted traits in plants. Numerous plants, including energy crops, known for their potential to tolerate, immobilize, and stabilize inorganic and organic pollutants, have already been edited using different CRISPR systems. Moreover, a large array of genes responsible for increased metal tolerance, metal uptake and hyperaccumulation have already been identified. Thus, the CRISPR-mediated genome reprogramming of plants, including its use in gene expression regulation through transcriptional repression or activation (CRISPRi and CRISPRa), could be of paramount importance for phytoremediation. The simplicity, inexpensiveness, and capabilities of this gene editing technique could soon be used to enhance plants and bacteria involved in phytotechnologies, such as phystabilization, phytoextraction, phytomining, phytovolatilization, and bio-energy generation. In this brief viewpoint piece, we posit some of the potential benefits of CRISPR for phytoremediation.
- Plant growth–promoting rhizobacteria-assisted phytoremediation of mine soilsPublication . Novo, Luís A. B.; Castro, Paula M. L.; Alvarenga, Paula; Silva, Eduardo F. daThe ever growing buildup of mine waste across the planet has become a global concern. Mine spoils often endanger surrounding ecosystems because toxic metals migrate through air, water, and soil, and ultimately enter the food chain. Yet, conventional techniques are usually impracticable because of the large amounts of mine waste, their cost and frequent hazardous side effects. Phytoremediation, a plant-based technique that allows immobilizing and/or extracting pollutants has shown promising results in the reclamation of mine tailings. Nevertheless, the need to enhance the efficiency of phytoremediation has prompted the adoption of auxiliary strategies such as the use of plant growth-promoting rhizobacteria (PGPR). PGPR are known to bestow a wide variety of beneficial effects such as increased growth, improved immunity, and the modification of metal phytoavailability in the rhizosphere, to name a few. In this chapter, we review the impact of PGPR on phytoremediation of mine soils to date and discuss future directions.
- Fitotecnologias: situação atual e perspectivas futurasPublication . Bernardino, Cassiano Augusto Rolim; Mahler, Claudio Fernando; Silva, Eduardo Ferreira da; Novo, Luís António BalreiraA degradação do meio ambiente, o aumento da urbanização, a elevada demanda energética, e as alterações climáticas, são atualmente alguns dos principais desafios do ser humano. Além disso, as atividades antropogênicas têm contribuído para a contaminação dos recursos naturais devido a geração, emissão e deposição de poluentes orgânicos e inorgânicos. Nesse sentido, um conjunto de técnicas alicerçadas no uso de plantas (fitotecnologias), têm ganhado grande visibilidade pois oferecem soluções ecológicas e sustentáveis para mitigar esses problemas. Esta revisão sumariza a situação atual dos principais tipos de fitotecnologias, das quais se destacam a fitorremediação, a fitomineração, a utilização da biomassa para produção de energia, e as coberturas verdes. Este trabalho apresenta ainda alguns dos desafios futuros das fitotecnologias, como a sua otimização através de técnicas avançadas de edição genética ou a articulação com as nanotecnologias.
- The effects of rhenium accumulation on Indian mustardPublication . Novo, Luís A. B.; Silva, Eduardo F.; Pereira, Andrea; Casanova, Alba; González, LuísRhenium(Re) is one of Earth’s scarcest and more largely scattered elements, with an estimate concentration of 0.4–0.6 μg kg−1 in the upper crust. Still, considerable concentrations of bioavailable ReO4 − ions are often found, particularly in copper-molybdenum mines, where their uptake by plants of these regions has been reported. Yet, the impact of Re on plants remains a question mark, as the only available knowledge derives from a limited investigation carried out over 60 years ago. The aim of this study was to evaluate the ecophysiological response of Brassica juncea, a species known to endure and accumulate various metals, to a broad range of Re concentrations. B. juncea plants were allowed to grow and on a substrate amended with KReO4 to attain soil Re levels ranging from 0 to 80 mg kg−1. Plants were collected 45 days after sowing for analysis. The results have shown that greater Re levels reduce growth, photosynthetic activity, soluble carbohydrate mobilization, and protein contents, and increase the plant’s oxidative stress (anthocyanins, H2O2, lipid peroxidation) and corresponding response (ascorbic acid, superoxide dismutase activity). Nevertheless, B. juncea exhibited a remarkable ability to endure and uptake Re, featuring shoot Re concentrations that ranged from 1615 to 24,987 mg kg−1 among the 5 and 80 mg kg−1 treatments.
