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- Optimization of the culture medium composition using response surface methodology for new recombinant cyprosin B production in bioreactor for cheese productionPublication . Sampaio, Pedro N.; Calado, Cecília R. C.; Sousa, Lisete; Bressler, David C.; Pais, Maria Salomé; Fonseca, Luís PinaThe optimization of culture medium compositionwas carried out for improvement the recombinantcyprosin B production, an enzyme with high milk-clottingactivity. Response surface methodology (RSM) wasapplied to evaluate the effect of variables namely glucose,yeast extract (YE) and bactopeptone present in the culturemedium, used for recombinant cyprosin B production bytransformed Saccharomyces cerevisiae BJ1991 strain inshake-flask and bioreactor culture conditions. The centracomposite experimental design (CCD) was adopted toderive a statistical model for optimizing the composition ofthe fermentation medium. The optimal concentration estimatedfor each variable related to a theoretical maximumof cyprosin B activity (488 U mL-1) was 30 g L-1 glucose,15 g L-1 YE and 27 g L-1 bactopeptone. The optimizedmedium composition, based on empirical model, ledto a cyprosin B activity of 519 U mL-1, which correspondsto an increase of 46%. The fermentation using optimizedculture medium in a 5-L bioreactor allowed a significantincrease in biomass (82%) and recombinant cyprosin Bproduction (139%). The improvement in the recombinantcyprosin B production after optimization process can beconsidered adequate for large-scale applications, and theclotting activity of cyprosin B account for their use inindustrial cheese making.
- Validation of the falls efficacy scale - international in a sample of Portuguese elderlyPublication . Marques-Vieira, Cristina Maria Alves; Sousa, Luís Manuel Mota de; Sousa, Lisete Maria Ribeiro de; Berenguer, Sílvia Maria Alves CaldeiraOBJECTIVE: to translate and adapt Falls Efficacy Scale - International (FES-I). To analyze the psychometric properties of the FES-I Portugal version. METHOD: psychometric study. Sample consisting of 170 elderly people residing in the Autonomous Region of Madeira. A two- part form was used (sociodemographic characterization and FES-I Portugal). The cross-cultural adaptation was performed and the following psychometric properties were evaluated: validity (construct, predictive, and discriminant), reliability (Cronbach's alpha), and inter-rater reliability. RESULTS: the results allow us to verify a dimension of less demanding physical activities and another of more demanding physical activities. The inter-rater reliability study was 0.62, with an interclass correlation coefficient of 0.859, for a 95% confidence interval. The internal consistency of the Portuguese version was 0.962. CONCLUSION: the validity and reliability of the FES-I Portugal are consistent with the original version and proved to be appropriate instruments for evaluating the "impaired walking" and "risk of falls" nursing diagnoses in the older people.
- The nursing diagnosis ‘impaired walking’ in elderly: systematic literature reviewPublication . Marques-Vieira, Cristina Maria Alves; Sousa, Luís Manuel Mota de; Sousa, Lisete Maria Ribeiro de; Caldeira, SílviaWalk is an activity that requires different skills and can be highly complex particularly for the elderly. The aim was to identify the defined characteristics and related factors of the nursing diagnosis impaired walking in elderly. A Systematic literature review, based on a search done between January and March 2014, in the electronic platforms EBSCO Host®, SCOPUS and ISI, and using the search strategy walk* OR gait AND Nurs*. A sample of 36 studies was obtained. A total of 17 defined characteristics and 34 related factors were identified. Among all, nine defined characteristics and 20 related factors are not listed in the diagnosis of NANDA International. This research identified new defined characteristics and related factors not listed in NANDA International. This study is a contribution to the development of the taxonomy of NANDA International, which should represent nursing knowledge, and this highlights some implications for clinical practice, education and further research.