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- 1,3-Propanediol continuous production by Clostridium butyricum VP1 1718: effect of dilution rate and substrate concentrationPublication . Gonzalez-Pajuelo, M.; Ribeiro-Cruz, A. P.; Sousa- Monagas, C. M.; Andrade, J. C.; Vasconcelos, I.
- Fermentation of Low Grade Glycerol by Clostridium butyricum and Clostridum acetobutyllcum StrainsPublication . Gonzalez-Paluelo, M.; Andrade, J.C.; Vasconcelos, I.
- Antimicrobial resistance patterns inEnterobacteriaceae isolated froman urbanwastewater treatment plantPublication . Silva, Miguel Ferreira da; Vaz-Moreira, Ivone; Gonzalez-Pajuelo, Maria; Nunes, Olga C.; Manaia, Célia M.Over 18 months, enterobacteria were isolated from the raw (189 isolates) and treated (156 isolates) wastewater of a municipal treatment plant. The isolates were identified as members of the genera Escherichia (76%), Shigella (7%), Klebsiella (12%) and Acinetobacter (4%). Antimicrobial susceptibility phenotypes were determined using the agar diffusion method for the antibiotics amoxicillin, gentamicin, ciprofloxacin, sulfamethoxazole/trimethoprim, tetracycline and cephalothin, the disinfectants hydrogen peroxide, sodium hypochlorite, quaternary ammonium/formaldehyde and iodine, and the heavy metals nickel, cadmium, chromium, mercury and zinc. Class 1 integrons were detected by PCR amplification using the primers CS5 and CS3. Compared with the raw influent, the treated wastewater presented higher relative proportions of Escherichia spp. isolates resistant to ciprofloxacin and cephalothin (Po0.0001 and Po0.05, respectively). Except for mercury, which showed a positive correlation with tetracycline and sulfamethoxazole/trimethoprim, no significant positive correlations were observed between antibiotic, disinfectant and heavy metal resistance. The variable regions of class 1 integrons, detected in c. 10% of the Escherichia spp. isolates, contained predominantly the gene cassettes aadA1/dhfrI.
- Glycerol bioconversion to 1,3-propanediol by Clostridium butyricum in continuous cultures with cell recyclePublication . Gonzalez-Pajuelo, M.; Andrade, J.C.; Vasconcelos, I.