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Orientador(es)
Resumo(s)
Over 18 months, enterobacteria were isolated from the raw (189 isolates) and
treated (156 isolates) wastewater of a municipal treatment plant. The isolates were
identified as members of the genera Escherichia (76%), Shigella (7%), Klebsiella
(12%) and Acinetobacter (4%). Antimicrobial susceptibility phenotypes were
determined using the agar diffusion method for the antibiotics amoxicillin,
gentamicin, ciprofloxacin, sulfamethoxazole/trimethoprim, tetracycline and cephalothin,
the disinfectants hydrogen peroxide, sodium hypochlorite, quaternary
ammonium/formaldehyde and iodine, and the heavy metals nickel, cadmium,
chromium, mercury and zinc. Class 1 integrons were detected by PCR amplification
using the primers CS5 and CS3. Compared with the raw influent, the treated
wastewater presented higher relative proportions of Escherichia spp. isolates
resistant to ciprofloxacin and cephalothin (Po0.0001 and Po0.05, respectively).
Except for mercury, which showed a positive correlation with tetracycline and
sulfamethoxazole/trimethoprim, no significant positive correlations were observed
between antibiotic, disinfectant and heavy metal resistance. The variable regions of
class 1 integrons, detected in c. 10% of the Escherichia spp. isolates, contained
predominantly the gene cassettes aadA1/dhfrI.
Descrição
Palavras-chave
Enterobacteriaceae Antibiotic Resistance Disinfectants Heavy metals Class 1 integron
Contexto Educativo
Citação
SILVA, Miguel Ferreira da...[et al] - Antimicrobial resistance patterns in Enterobacteriaceae isolated from an urban wastewater treatment plant. FEMS Microbiology Ecology. ISSN 0168-6496. Vol. 60 (2007), p.166–176
Editora
Blackwell Publishing