Browsing by Issue Date, starting with "2001"
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- ExcluídoPublication . Cunha, Jorge Teixeira da
- Microbiological profile in sourdough: evolution during refrigerated storage.Publication . Rocha, João M.; Malcata, F. Xavier
- O princípio de incerteza de W. Heisenberg: física e filosofiaPublication . Meneses, Ramiro Délio Borges de
- Hydrolysis of whey proteins by proteases extracted from Cynara cardunculus and immobilized onto highly activated supportsPublication . Lamasa, Estela M.; Barrosa, Rui M.; Balcão, Victor M.; Malcata, F. XavierBlends of cardosins A and B, enzymes present in aqueous extracts of the flowers of the thistle (Cynara cardunculus L.), have for long been used as rennets by the cheesemaking industry in the Iberian Peninsula. These dimeric proteases are present in the stigmæ and stylets of said flowers, and are thought to play a role in sexual reproduction of the plant. In the present research effort, production of cardosin derivatives (starting from a crude extract), encompassing full stabilization of their dimeric structure, has been attempted via covalent, multi-subunit immobilization onto highly activated agarose-glutaraldehyde supports. Boiling such enzyme derivatives in the presence of sodium dodecyl sulfate and β-mercaptoethanol did not lead to leaching of enzyme, thus proving the effectiveness of the attachment procedure. Furthermore, derivatives prepared under optimal conditions presented ca. half the specific activity of the enzyme in soluble form, and were successfully employed at lab-scale trials to perform (selective) hydrolysis of α-lactalbumin, one of the major proteins in bovine whey.
- Artigo 385º (Abandono de funções)Publication . Cunha, José Damião da
- A questão do mal em Paul RicceurPublication . Martins, Maria Manuela Brito
- Determination of carotenoid profiles in grapes, musts, and fortified wines from Douro varieties of vitis viniferaPublication . Pinho, Paula Guedes de; Ferreira, António C. Silva; Pinto, Manuela Mendes; Benitez, Juan Gomez; Hogg, Timβ-Carotene and six xanthophylls (lutein, neoxanthin, violaxanthin, luteoxanthin, cryptoxanthin, and echinenone) have been identified and semiquantitatively or quantitatively determined in musts and port wines for the first time. An HPLC method was developed and compared with that of one based on thin layer cromatography with scanning densitometry. The most abundant carotenoids present in red grape varieties are β-carotene and lutein. In wines, significant quantities of violaxanthin, luteoxanthin, and neoxanthin were found. This study was done with berries (skin and pulp), musts, and fortified wines. Some experiments were performed to follow carotenoid content from grapes to wines. Although the levels of β-carotene and lutein found in fortified wines were lower than those found in musts, other xanthophylls, such as neoxanthin, violaxanthin, and luteoxanthin, exist in appreciable amounts in young ports.
- A alma e o corpo em fragmentos de Safo: traduções e adaptaçõesPublication . Pulquério, Manuel
- O pensamento filosófico de António José BrandãoPublication . Sousa, Ana Paula Loureiro de
- Artigo 317º (Espionagem)Publication . Cunha, Maria da Conceição