Browsing by Issue Date, starting with "1994"
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- Ocorrência de Listeria spp. e Listeria monocytogenes em pescado fumado a frio em PortugalPublication . Duarte, Gabriela; Velho, Manuela Vaz; Gibbs, Paul
- O poema Fontellum de António de CabedoPublication . Couto, Aires Pereira do
- Carbodiimide modification enhances activity of pig pancreatic phospholipase A2Publication . Ferreira, João Paulo M.; Sasisekharan, Ram; Louie, Otway; Langer, RobertPig phospholipase A2, pig iso-phospholipase A2 and bovine pancreatic phospholipase A2 were reacted in solution with 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide, in the presence of N-hydroxysulfosuccinimide, at pH 7. The influence of micellar protectants was analyzed. In the presence of n-hexadecylphosphocholine, the losses of activity in micellar diheptanoyl-lecithin were 80, 35, and 10% in bovine phospholipase A2, pig iso-phospholipase A2, and pig phospholipase A2, respectively. With 1-oleoylglycerophosphocholine, the bovine enzyme lost 40% activity, but the pig enzyme was activated sevenfold. The modified pig enzyme showed pre-micellar activation on monomeric diheptanoyl-lecithin, and either reduced or increased activities on mixed micelles of bile salt with egg phosphatidylcholine, depending on the composition of the micelles. This activation is consistent with previous protein-engineering studies of pig pancreatic phospholipase A2. In this study, we present new information concerning the specificity and interfacial recognition behaviour of this enzyme in relation to this activation.
- Rheological description at the minimum gelling conditions of kappa-carrageenan/locust bean gum systemsPublication . Fernandes, Paulo B.; Gonçalves, M.P.; Doublie, Jean-LouisKappa-carrageenan/locust bean gum interactions at the minimum gelling concentration have been studied by dynamic and viscosity measurements at 15°C. Both rheological methods used have been shown to be complementary. The existence of a weak network structure exhibiting thixotropic behaviour has been observed. The formation of the weak network is discussed.
- Roma-Portugal, impressões de um paísPublication . Cardoso, A. Pinto
- Hannah Arendt e as origens do totalitarismoPublication . Andrade, Inês Madeira de
- A visão das origens em Gen 2,4b-3,24 como coerência temática e unidade literáriaPublication . Vaz, Armindo dos Santos
- Ser e estar perante a morte no Porto dos séculos XIX e XX: reflexos no património cemiterialPublication . Sousa, Gonçalo de Vasconcelos e
- Bibliotecas monásticas e culturaPublication . Ganho, Maria de Lourdes Sirgado