Percorrer por autor "Rodrigues, Francisco A. P."
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- Coaxial 3D printing: synergistic approach of natural and synthetic biomaterials for knee meniscus replacementPublication . Rodrigues, Francisco A. P.; Araújo, Marco; Granja, Pedro L.; Oliveira, Ana Leite; Costa, João B.
- Detergent-free supercritical CO2–assisted protocol for the production of sustainable and highly preserved decellularized porcine meniscus for orthopedic applicationsPublication . Ho, Chou I.; Rodrigues, Francisco A. P.; Reis, Mariana S.; Ribeiro, Viviana P.; Oliveira, Ana Leite; Costa, João B.Introduction & Objectives Meniscal injuries occur approximately 66 to 70 per 100,000 individuals annually (Fig. 1), potentially leading to the development of osteoarthritis (OA) or other degenerative cartilage disease in 10 to 20 years. One of the conventional treatments is meniscal allograft transplantation. However, its limitations constrain its comprehensive application in the healthcare system.
- Detergent-free supercritical CO2–assisted protocol for the production of sustainable and highly preserved decellularized porcine meniscus for orthopedic applicationsPublication . Ho, Chou I.; Rodrigues, Francisco A. P.; Reis, Mariana S.; Ribeiro, Viviana P.; Oliveira, Ana L.; Costa, João B.
- Detergent-free supercritical CO2–assisted protocol for the production of sustainable and highly preserved decellularized porcine meniscus for orthopedic applicationsPublication . Ho, Chou I.; Rodrigues, Francisco A. P.; Reis, Mariana S.; Ribeiro, Viviana P.; Oliveira, Ana Leite; Costa, João B.
- Going green and mimetic: new ECM-based hydrogel for meniscus regeneration using supercritical CO2-assisted decellularizationPublication . Ho, Chou I.; Rodrigues, Francisco A. P.; Reis, Mariana S.; Sousa, Clara; Ribeiro, Viviana P.; Oliveira, Ana L.; Costa, João B.Meniscal injuries and its subsequent progression to osteoarthritis represent a major clinical challenge, as current treatments often fail to achieve effective and complete functional restoration. An alternative regenerative approach is tissue engineering using decellularized extracellular matrix (dECM), which aims to restore meniscal structure and function. Porcine meniscus, an abundant byproduct of meat production, offers a readily available source for this purpose. Unlike conventional decellularization methods that are harsh, time-consuming, and detrimental to extracellular matrix (ECM) integrity, a detergent-free decellularization protocol utilizing supercritical carbon dioxide (scCO2) fluid was developed. This sustainable and minimally-invasive process was employed in cyclic pressurization-depressurization dynamics and scCO2 fluid was applied for sterilization by incorporating oxidative additives. Quantitative and qualitative analyses using quantification extraction kits and Fourier transform infrared (FTIR) spectroscopic analysis, presented as principal component analysis (PCA) and loading plots through a Machine Learning Toolbox, demonstrated substantial preservation of key ECM components. Sterilization efficiency was also confirmed using turbidity tests. dECM residual DNA content results fell within the range of general benchmark of 50ng/mg of dry tissue and the endotoxin levels, recently recognized as critical cause of host responds and regenerative outcomes [1], were below 20 endotoxin units (EU). The dECM showed cytocompatibility in vitro and an injectable hydrogel was successfully developed. The dECM hydrogel presented adhesive properties and was optimized to be used as a bioink for 3D bioprinting approaches.
- In-situ fibroin conformational change using a coaxial-printing strategy for knee meniscus replacementPublication . Rodrigues, Francisco A. P.; Oliveira, Ana Leite; Costa, João B.
- In-situ silk fibroin conformational change using a coaxial-printing strategy for knee meniscus replacementPublication . Rodrigues, Francisco A. P.; Oliveira, Ana Leite; Costa, João B.
- Innovative sampling strategies for trace metals analysis in water monitoringPublication . Ribas, Tânia C. F.; Pereira, Mafalda G.; Rodrigues, Francisco A. P.; Mesquita, Raquel B. R.; Rangel, António O. S. S.
- Molecules in motion: unravelling the dynamics of vascularization control in tissue engineeringPublication . Rodrigues, Francisco A. P.; Oliveira, Claudia S.; Sá, Simone C.; Tavaria, Freni K.; Lee, Sang Jin; Oliveira, Ana L.; Costa, João B.Significant progress has been made in tissue engineering (TE), aiming at providing personalized solutions and overcoming the current limitations of traditional tissue and organ transplantation. 3D bioprinting has emerged as a transformative technology in the field, able to mimic key properties of the natural architecture of the native tissues. However, most successes in the area are still limited to avascular or thin tissues due to the difficulties in controlling the vascularization of the engineered tissues. To address this issue, several molecules, biomaterials, and cells with pro- and anti-angiogenic potential have been intensively investigated. Furthermore, different bioreactors capable to provide a dynamic environment for in vitro vascularization control have been also explored. The present review summarizes the main molecules and TE strategies used to promote and inhibit vascularization in TE, as well as the techniques used to deliver them. Additionally, it also discusses the current challenges in 3D bioprinting and in tissue maturation to control in vitro/in vivo vascularization. Currently, this field of investigation is of utmost importance and may open doors for the design and development of more precise and controlled vascularization strategies in TE.
- A novel sampling strategy for metal ions determination in water: application to the determination of cadmiumPublication . Rodrigues, Francisco A. P.; Ribas, Tânia C. F.; Mesquita, Raquel B. R.; Rangel, António O. S. S.This work proposes a novel, portable, and reusable device for in situ water sampling, sample preparation, and analyte enrichment in a single step. For this purpose, a commercial cationic exchange resin (Chelex 100), was used to obtain a solid-phase extraction column for cadmium (II) retention and enrichment. The optimization of key parameters such as sample volume, reagents concentration, and flow-rate, was performed to maximize cadmium retention and elution efficiency. The method was applied to real samples, and the results agreed with those obtained with reference methodologies, with no significant differences (< 10%). Additionally, recovery rates of 97.8 ± 3.5% were achieved. This method integrates, in one step, the sampling and sample preparation procedures and employs reusable devices, contributing to providing a sustainable solution for metal ions monitoring in aquatic environments.