Browsing by Author "Pintado, Ana"
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- Carnosic acid production from sugarcane syrup by engineered yeast in fed-batch fermentationPublication . Carsanba, Erdem; Fernandes, Sara; Beato, Felipe; Carvalho, Luís Carlos; Pintado, Ana; Lopes, Ana; Ribeiro, Mónica; Leal, Tânia; Pintado, Manuela; Oliveira, CarlaPhenolic diterpene carnosic acid (CA) is widely used in the food, nutritional health, and cosmetic industries due to its antioxidative and antimicrobial properties. This work aimed to overproduce CA in Saccharomyces cerevisiae from sugarcane syrup in fed-batch 2 L bioreactor fermentation. A geranylgeranyl diphosphate (GGPP)-producing strain modified with genes encoding the enzymes copalyl diphosphate synthase (Pv.CPS), miltiradiene synthase (Ro.KSL2), hydroxy ferruginol synthase (Ro.HFS), CA synthase (Ro.CYP76AK8), CYP reductase (At.ATR1), and transketolase (TKL1) was used. Lowering the feed rate from 12–26 g/L/h to 7–8 g/L/h, and the use of a dynamic dissolved oxygen (DO) trigger (min. 10%, max. 40%, threshold 70%) instead of a DO trigger of 30%, enhanced CA production by 27%. As a result, the highest CA titer ever reported to date, 191.4 mg/L, was obtained in 4-day fermentation. This study shows the feasibility of engineered yeast to produce CA from the sustainable feedstock sugarcane syrup.
- Chaves thermal spring water impact on skin health: potential cosmetic applicationPublication . Pinto-Ribeiro, Inês; Castro, Cláudia; Rocha, Pedro Emanuel; Carvalho, Maria João; Pintado, Ana; Mendes, Adélia; Pedrosa, Sílvia Santos; Capeto, Paula; Azevedo-Silva, João; Oliveira, Ana L. S.; Pintado, Manuela; Madureira, Ana RaquelSince ancient times, thermal spring water has been proven to be beneficial to the skin and to improving dermatologic disorders, explaining its incorporation into cosmetic formulations as an active ingredient. Chaves thermal spring water, from northern Portugal, has been used as a local spa since Roman times, and its customers are satisfied with its medicinal quality. Despite the lack of published evidence on its specific effects on the skin, this study evaluates the potential of using Chaves thermal water as a cosmetic ingredient. The physiochemical composition demonstrated that Chaves thermal spring water is low-mineralized water, and its major components are sodium, potassium, silicon, and calcium. In vitro experiments demonstrated that this low mineralization might explain the absence of antioxidant and antiaging potential, and the maintenance of collagen and fibronectin levels. The quantification of the IL-6 levels showed that Chaves thermal spring water could be used as an anti-inflammatory product, suggesting its use by individuals with skin diseases. In agreement with this result, in vivo experiments revealed that Chaves thermal spring water improved the integrity of the skin barrier and preserved the skin microbial community. Overall, the present work suggests that Chaves thermal spring water might be used as a cosmetic product.