Percorrer por autor "Oliveira, M."
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- Clean label alternatives to the use of nitrite in cooked ham: results of a pilot studyPublication . Carvalho, T. Bento de; Oliveira, M.; Komora, N.; Teixeira, P.Introduction: Environmental and health concerns over the use and consumption of artificial additives like nitrite have prompted consumer wishes and desire for “cleaner” products containing natural ingredients whose names they know and understand. Objectives: The aim of this work was to assess if natural sources of nitrate in combination with nitrate-reducing starter cultures would be good substitutes of chemical nitrite in cooked ham. Methodology: Four cooked hams, combining rich nitrate vegetable sources with two different nitrate- reducing commercial starter cultures were manufactured at pilot scale by Primor. Results and discussion: With the exception of Enterobacteriaceae, which were not found in the control ham, all other microbiological parameters were similar for the various hams. The challenge test showed that at abuse temperature, L. monocytogenes increased 1 to 2 log cycles when compared to storage at 4 ℃. The colour, texture, pH and aw values were similar for the four test hams and the control throughout storage (28 days). Conclusions: These preliminary results demonstrate the potential of using natural sources of nitrates combined with nitrated-reducing starters as a clean label alternative to the use of nitrite in cooked ham, maintaining microbiological safety and organoleptic properties.
- Here4You-Footsteps... building the future: an action research project to prevent tobacco consumption in the 3rd CyclePublication . André, C.; Amendoeira, J.; Figueiredo, M. C.; Martins, M. R.; Pinto, C.; Nunes, M.; Rainha, L.; Godinho, A.; Ferreira, M.; Pintor, M.; Oliveira, M.Introduction Tobacco is the first preventable cause of morbidity and mortality, and should be targeted at younger age groups, with the school being considered as a privileged space for this intervention. The project “ Here4You - Footsteps…Building the future – Health Promoting School “ aims to enable 3rd cycle students to make responsible decision making in the prevention of tobacco consumption by building knowledge, attitudes, skills regarding healthy lifestyles, and behaviours related to risk factors associated with tobacco use. Objectives To disclose the gains from the development of the project. Methods Project developed in partnership between the School of Health of Santarém, as Health Promotion School - Health Indicators Monitoring Unit, and the Community Care Unit of Chamusca/Golegã. It is inserted in the methodology of Action Research: Diagnosis, Planning, Intervention and Evaluation, in the 7th and 8th years, from 2015/2016 until 2018/2019. The Global Youth Tobacco Survey, Portuguese version, was used at the beginning (7th year) and at the end of the intervention (8th year). Results 460 students were interviewed, with 279 sessions of health education in the classroom context. All the sessions were evaluated, using a questionnaire that integrates questions related to the contents and a grid of opinion about the session, constituted by 5 items, with scores between [5; 25]. In the last edition of the project, 13% of 7th graders said they had already smoked and, in the previous one, 10% of the students said they smoked, the lowest value found. Conclusions In order to foster complementarity and convergence solutions to generate positive synergies, the intervention occurred in the classroom in the different disciplines. Obtaining health outcomes implies a consistent and continuous intervention that accompanies the students throughout their formative course. With the GYTS it has been possible to evaluate the impact of the developed intervention.
- In-situ conversion of natural sources of nitrate for clean labelling meat products: cooked ham case studyPublication . Carvalho, T. Bento de; Oliveira, M.; Komora, N.; Teixeira, P.Introduction: Clean labelling of meat products has emerged as a promising marketing trend in the industry. Ingredient lists with recognizable ingredients and minimal processing have been preferred by consumers in recent years, with increasing demand. Objectives: This study firstly aimed to assess the behaviour of three pathogens commonly associated with ham products throughout storage at refrigeration (4 ºC) and abuse (10 ºC) temperature. Secondly, sensory analysis to evaluate differences between hams was carried out. Results and discussion: When inoculated in the products, L. monocytogenes reached similar values in both control and the test hams. A 2-log difference was observed between the two storage temperatures, being that at 10 °C bacterial counts were higher. No differences between C. sporogenes and C. perfringens counts were observed for both temperatures and between test ham samples and control sample (p > 0.05). No differences between samples, except ham sample C, and control were found in sensory analysis, proving the promising commercial potential of the restructured hams. Conclusion: These results show the promising application of this technology in the meat industry, having in mind consumers wishes and demand for more nutritious and natural ingredients in day-to-day foods.
- Listeria monocytogenes gut interactions and listeriosis: gut modulation and pathogenicityPublication . Oliveira, M.; Barbosa, J.; Teixeira, P.Following ingestion via contaminated food, Listeria monocytogenes faces multiple hurdles through the human digestive system, thereby influencing its capacity to cause infection. This review provides a comprehensive overview of the multifaceted mechanisms employed by L. monocytogenes to overcome gastrointestinal hurdles and interact with the host's microbiota, facing chemical and physical barriers such as saliva, stomach acidity, bile salts and mechanical clearance. Proposed evasion strategies will be highlighted, exploring the bacteriocins produced by L. monocytogenes, such as the well-described bacteriocin Listeriolysin S (LLS), a bacteriocin that inhibits inflammogenic species – Lmo2776, and a phage tail-like bacteriocin, monocin. The competitive dynamic interactions within the gut microbiota, as well as the modulation of microbiota composition and immune responses, will also be explored. Finally, the adhesion and invasion of the intestinal epithelium by L. monocytogenes is described, exploring the mechanism of pathogenesis, biofilm and aggregation capacities and other virulence factors. Unlike previous reviews that may focus on individual aspects of L. monocytogenes pathogenicity, this review offers a holistic perspective on the bacterium's ability to persist and cause infection, integrating information about survival strategies, including bacteriocin production, immune modulation, and virulence factors. By connecting recent findings on microbial interactions and infection dynamics, this review incorporates recent developments in the field and connects various lines of research that explore both host and microbial factors influencing infection outcomes.
- Proyecto ReCROP: mejorando la diversidad microbiana del suelo en agroecosistemas mediterráneosPublication . Epelde, L.; Anitua, A.; Anza, M.; Garbisu, C.; Prieto-Fernández, Á.; Martínez, M. C. Monterroso; Rodríguez-Garrido, B.; Cepeda, C. Trasar; González-Prieto, S.; Cortet, J.; Delcourt, N.; Kadiri, N.; Vergnes, A.; Roussel, S.; Blayac, T.; Lavaine, E.; Boularbah, A.; Benidire, L.; El Balghiti, F. Z.; Allani, M.; Soufi, S.; Bettaieb, T.; Sahli, A.; Semida, W. M.; Abd El-Mageed, T. A.; Abdulfattah, M. A.; Alves, R.; Oliveira, M.; Santos, C.; Tassi, E.; Bretzel, F.; D'Acqui, L. P.; Pini, R.; di Lonardo, S.; Cortez, J.; Cardoso, E.; Moreira, H.; Pereira, S. I. A.; Castro, P. M. L.
- Proyecto ReCROP: mejorando la diversidad microbiana del suelo en agroecosistemas mediterráneosPublication . Epelde, L.; Anitua, A.; Anza, M.; Garbisu, C.; Prieto-Fernández, Á.; Martínez, M. C. Monterroso; Rodríguez-Garrido, B.; Cepeda, C. Trasar; González-Prieto, S.; Cortet, J.; Delcourt, N.; Kadiri, N.; Vergnes, A.; Roussel, S.; Blayac, T.; Lavaine, E.; Boularbah, A.; Benidire, L.; El Balghiti, F. Z.; Allani, M.; Soufi, S.; Bettaieb, T.; Sahli, A.; Semida, W. M.; Abd El-Mageed, T. A.; Abdulfattah, M. A.; Alves, R.; Oliveira, M.; Santos, C.; Tassi, E.; Bretzel, F.; D'Acqui, L. P.; Pini, R.; di Lonardo, S.; Cortez, J.; Cardoso, E.; Moreira, H.; Pereira, S. I. A.; Castro, P. M. L.
- ReCROP - bioinocula and CROPping systems: an integrated biotechnological approach for improving crop yield, biodiversity and REsilience of Mediterranean agro-ecosystemsPublication . Delcourt, N.; Cortet, J.; Vergnes, A.; Pereira, S. I. A.; Prieto-Fernández, A.; Epelde, L.; Roussel, S.; Boularbah, A.; D'Acqui, L. P.; Semida, W. M.; Sahli, A.; Alves, R.; Oliveira, M.; Castro, Paula M. L.
- ReCROP: bioinocula and CROPping systems: an integrated biotechnological approach for improving crop yield, biodiversity and REsilience of Mediterranean agro-ecosystemsPublication . Delcourt, N.; Cortet, J.; Vergnes, A.; Pereira, S. I. A.; Prieto-Fernández, A.; Epelde, L.; Roussel, S.; Boularbah, A.; D'acqui, L. P.; Semida, W. M.; Sahli, A.; Alves, R.; Oliveira, M.; Castro, P. M. L.The Mediterranean economy is highly dependent on agriculture. However, agricultural sustainability and productivity in this region is under serious threat due to climate change and the depletion of water resources. This is worsened by poor management practices, such as the overuse of chemical fertilizers, pesticides, overgrazing and monoculture farming. Recent climate change models indicate that European and Northern African regions will undergo extreme climatic events throughout the year, this will negatively impact crop yield and productivity. Summer droughts and heat waves periods will increase for most parts of Europe, as well as short intense rain events. Preserving and improving productive agricultural land in this region is vital, especially through the application of sustainable soil and crop management practices that promote soil fertility and water conservation; this will improve resilience to degradation and to extreme climatic events. ReCROP is a European project that aims to identify sustainable and resilient agricultural production systems in the Mediterranean region through the combined use of biotechnological tools, such as bioinoculants, and environmentally friendly agronomic practices. ReCROP will assess different agroecosystems with key local crops (i.e vineyards, maize and aromatic/medicinal plants) of the Mediterranean region under field conditions to help improve crop resilience, yield, water conservation and soil health under the current scenario of climate change. Soil organisms play a key role in ecosystem processes, leading to essential soil functions and are used as bioindicators of soil quality. Their monitoring is crucial to assess the impact of beneficial agricultural practices on soil functioning. One of the goals of ReCROP will be to evaluate the beneficial impact of different agricultural practices on the structural and functional soil diversity at different levels of the soil food web. The macrofauna and mesofauna (i.e springtails and mites) as well as microbial biomass, activity and biodiversity of soil microbial communities (bacteria, archaea, fungi) will be monitored with a special effort to produce a multitaxa index of soil biological quality. This work will contribute to identify which practices are beneficial for the biodiversity of Mediterranean agricultural soils, thus providing resistance and resilience, in terms of soil functioning and against soil disturbances.
- Sustainable solutions for mediterranean agroecosystems through enhanced soil microbial diversityPublication . Epelde, L.; Anitua, A.; Lavín, J.L.; Garbisu, C.; Prieto-Fernández, Á.; Monterroso Martínez, M. C.; Rodríguez-Garrido, B.; Cepeda, C. Trasar; González-Prieto, S.; Cortet, J.; Delcourt, N.; Kadiri, N.; Vergnes, A.; Roussel, S.; Blayac, T.; Lavaine, E.; Boularbah, A; Benidire, L.; Balghiti, F. Z. El; Allani, M.; Soufi, S.; T., Bettaieb; Sahli, A.; Semida, W. M.; Abd El-Mageed, T. A.; Abdulfattah, M.A.; Alves, R.; Oliveira, M.; Santos, C.; Tassi, E.; Bretzel, F.; D'Acqui, L. P.; Pini, R.; di Lonardo, S.; Cortez, J.; Cardoso, E; Moreira, H.; Pereira, S.I.A.; Castro, P. M. L.