Percorrer por autor "Nunes, Olga"
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- Crop rotation and cropping phase in rice paddies and their relationship with variations of the soil microbial communitiesPublication . Lopes, Ana Rita Rego; Bello, Diana; Prieto-Fernández, Ángeles; Trasar-Cepeda, Carmen; Gil-Sotres, Fernando; Leirós, Maria; Manaia, Célia M.; Nunes, Olga
- Fate of pseudomonas aeruginosa and blaVIM in soil under selective pressure by copper and zincPublication . Fortunato, Gianuario; Vaz-Moreira, Ivone; Nunes, Olga; Manaia, Célia
- A global multinational survey of cefotaxime-resistant coliforms in urban wastewater treatment plantsPublication . Marano, Roberto B.M.; Fernandes, Telma; Manaia, Célia M.; Nunes, Olga; Morrison, Donald; Berendonk, Thomas U.; Kreuzinger, Norbert; Tenson, Tanel; Corno, Gianluca; Fatta-Kassinos, Despo; Merlin, Christophe; Topp, Edward; Henn, Leonie; Scott, Andrew; Heß, Stefanie; Slipko, Katarzyna; Laht, Mailis; Kisand, Veljo; Cesare, Andrea Di; Karaolia, Popi; Michael, Stella G.; Petre, Alice L.; Rosal, Roberto; Pruden, Amy; Riquelme, Virginia; Agüera, Ana; Esteban, Belen; Luczkiewicz, Aneta; Kalinowska, Agnieszka; Leonard, Anne; Gaze, William H.; Adegoke, Anthony A.; Stenstrom, Thor A.; Pollice, Alfieri; Salerno, Carlo; Schwermer, Carsten U.; Krzeminski, Pawel; Guilloteau, Hélène; Donner, Erica; Drigo, Barbara; Libralato, Giovanni; Guida, Marco; Bürgmann, Helmut; Beck, Karin; Garelick, Hemda; Tacão, Marta; Henriques, Isabel; Martínez-Alcalá, Isabel; Guillén-Navarro, Jose M.; Popowska, Magdalena; Piotrowska, Marta; Quintela-Baluja, Marcos; Bunce, Joshua T.; Polo-López, Maria I.; Nahim–Granados, Samira; Pons, Marie-Noëlle; Milakovic, Milena; Udikovic-Kolic, Nikolina; Ory, Jérôme; Ousmane, Traore; Caballero, Pilar; Oliver, Antoni; Rodriguez-Mozaz, Sara; Balcazar, Jose L.; Jäger, Thomas; Schwartz, Thomas; Yang, Ying; Zou, Shichun; Lee, Yunho; Yoon, Younggun; Herzog, Bastian; Mayrhofer, Heidrun; Prakash, Om; Nimonkar, Yogesh; Heath, Ester; Baraniak, Anna; Abreu-Silva, Joana; Choudhury, Manika; P. Munoz, Leonardo; Krizanovic, Stela; Brunetti, Gianluca; Maile-Moskowitz, Ayella; Brown, Connor; Cytryn, EddieThe World Health Organization Global Action Plan recommends integrated surveillance programs as crucial strategies for monitoring antibiotic resistance. Although several national surveillance programs are in place for clinical and veterinary settings, no such schemes exist for monitoring antibiotic-resistant bacteria in the environment. In this transnational study, we developed, validated, and tested a low-cost surveillance and easy to implement approach to evaluate antibiotic resistance in wastewater treatment plants (WWTPs) by targeting cefotaxime-resistant (CTX-R) coliforms as indicators. The rationale for this approach was: i) coliform quantification methods are internationally accepted as indicators of fecal contamination in recreational waters and are therefore routinely applied in analytical labs; ii) CTX-R coliforms are clinically relevant, associated with extended-spectrum β-lactamases (ESBLs), and are rare in pristine environments. We analyzed 57 WWTPs in 22 countries across Europe, Asia, Africa, Australia, and North America. CTX-R coliforms were ubiquitous in raw sewage and their relative abundance varied significantly (<0.1% to 38.3%), being positively correlated (p < 0.001) with regional atmospheric temperatures. Although most WWTPs removed large proportions of CTX-R coliforms, loads over 103 colony-forming units per mL were occasionally observed in final effluents. We demonstrate that CTX-R coliform monitoring is a feasible and affordable approach to assess wastewater antibiotic resistance status.
- Microbiological quality of treated water by solar-driven oxidation processesPublication . Moreira, Nuno; Narciso-Da-Rocha, Carlos; Polo-López, Maria; Pastrana-Martínez, Luisa; Faria, Joaquim; Manaia, Célia M.; Fernández-Ibáñez, Pilar; Nunes, Olga; Silva, Adrián
- Study of bacterial microbiota over the course of tilapia growth cycle in an aquaponics systemPublication . Pablos, Cristina; Molina, Maria Dolores; Vaz-Moreira, Ivone; Silva, Maria Inês Leão; Nunes, Olga; Manaia, Célia; Marugán, JavierAquaponics combines hydroponic crop production with recirculating aquaculture. These systems comprise various compartments ( in this case: fish tank, biofilter, sump, hydroponic floating raft, settler, and aerobic digester provided by Green in Blue ). Efficient developing of aquaponics in terms of water quality, fish welfare, plant health and food safety requires a thorough understanding of the microbial composition for each compartment of the system . In this study 1m3 dechlorinated tap water aquaponic system stocked with 50 Nile tilapia ( Oreochromisniloticus L., stocked at 5.7 kg per system) and 72 lettuce plants (Lactuca sativa Batavian Red) for 22lettuce cycles of 5 weeks. The experiment was conducted in a small scale, closed-loop, simple recirculating aquaculture system (RAS) running for six months from 18 January ended on 21 July2023. Several physico -chemical water parameters were monitored throughout the cycle to follow the general evolution of the system and correlate potential microbiota variations with water parameters and establish connection to the nitrogen cycle. DNA sequencing results in Figure 1 clearly displays that aquaponics microbiome is complex and each compartment possesses its own bacterial community (p<0.05). An important genus in the biofilter is Nitrospira . This highlights its crucial role in nitrification as no Nitrobacter or Nitrosomonas is detected in the biofilter. Nocardia. A substantial proportion of denitrifying Flavobacterium genus common in soil, aquatic systems and plant-associated habitats were detected in sump. Also, Cetobacterium genus related to fish gut. As conclusion, metagenomics allows optimization of the operational parameters of aquaponics system to prevent pathogen proliferation and pathogen cross transfer among compartments, as well as to enhance efficient functioning of aquaponics biofilters.