Percorrer por autor "Mendes, Karina"
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- Antimicrobial properties and bioactivity potential of smart nanoparticles for dental applicationsPublication . Correia, Bruna L.; Malekshiri, Moslem; Bartolomeu, Maria; Silva, Virgília; Oliveira, Ana; Noites, Rita; Cardoso, Miguel; Mendes, Karina; Gomes, Ana T. P. C.; Duarte, Ana Sofia
- Consequences of early human saliva interaction on membranes for guided bone regenerationPublication . Kunrath, Marcel; Jolic, Martina; Giraldo-Osorno, Paula; Mendes, Karina; Rosa, Nuno; Dahlin, Christer
- D-2-hydroxyglutarate supports a tolerogenic phenotype with lowered major histocompatibility class II expression in non-malignant dendritic cells and acute myeloid leukemia cellsPublication . Hammon, Kathrin; Renner, Kathrin; Althammer, Michael; Voll, Florian; Babl, Nathalie; Decking, Sonja-Maria; Siska, Peter J.; Matos, Carina; Conejo, Zugey Elizabeth Cárdenas; Mendes, Karina; Einwag, Friederike; Siegmund, Heiko; Iberl, Sabine; Berger, Raffaela S.; Dettmer, Katja; Schoenmehl, Rebecca; Brochhausen, Christoph; Herr, Wolfgang; Oefner, Peter J.; Rehli, Michael; Thomas, Simone; Kreutz, MarinaD-2-hydroxyglutarate (D-2-HG) accumulates in primary acute myeloid leukemia (AML) patients with mutated isocitrate dehydrogenase (IDH) and other malignancies. D-2-HG suppresses antitumor T cell immunity but little is known about potential effects on non-malignant myeloid cells. Here we show that D-2-HG impairs human but not murine dendritic cell (DC) differentiation, resulting in a tolerogenic phenotype with low major histocompatibility (MHC) class II expression. In line, IDH-mutated AML blasts exhibited lower expression of HLA-DP and were less susceptible to lysis by HLA-DP-specific T cells. Interestingly, D-2-HG reprogrammed metabolism towards increased lactate production in DCs and AML besides its expected impact on DNA demethylation. Vitamin C accelerated DNA demethylation, but only the combination of vitamin C and glycolytic inhibition lowered lactate levels and supported MHC class II expression. Our results indicate an unexpected link between the immunosuppressive metabolites 2-HG and lactic acid and suggest a potentially novel therapeutic strategy with combinations of anti-glycolytic drugs and epigenetic modulators (hypomethylating agents) or other therapeutics for the treatment of AML.
- Exploring the inflammatory profile of homelessness population: a comprehensive analysis of individuals in two temporary shelters in LisbonPublication . Gomes, Ana T. P. C.; Mendes, Karina; Ferrito, Cândida; Andrade, Filipa; Neves-Amado, João; Resende, Ana; Santos, Paulo; Manso, Dina; Almeida, António; Vollrath, Antónia; Lopes, Rafaela; Barros, Marlene; Rosa, Nuno; Figueiredo, Amélia SimõesBackground: Homeless people are continuously facing adverse living conditions as poor access to basic nutrition, hygiene conditions and healthcare services, being at increased risk of severe infectious diseases as HIV and hepatitis as well as cardiovascular diseases and mental disorders. The characterization of homeless people’s health is fundamental to identify their health care needs. Considering that the aforementioned diseases are associated with chronic inflammatory processes, the main goal of this study was to characterize the inflammatory profile of a homeless population through quantification in saliva of a panel of inflammatory cytokines. Methods: The inflammatory profile was assessed in 114 individuals residing in two temporary shelters located in Lisbon and that accepted to participated in the study. Inflammatory proteins were quantified using a Multiplex Immunoassay approach. Data analysis was performed using the GraphPad Prism software and statistical significance among the groups was assessed using the nonparametric Mann–Whitney test. Results: Even though some protein levels might be masked by drug treatment, data analysis showed high levels of INF-ϒ, IL-10 and TNF-α in the infectious disease group, critical cytokines for the immune response against viruses and bacteria. Also, cytokines like IL-1β and IL-6 were detected at statistically significant levels in the cardiovascular disease group and all cytokines included in this study were quantified in the mental disorders group. Conclusion: These findings may help the healthcare services in the evaluation of treatment efficacy and disease monitoring, and in the development of effective public healthcare strategies and policy interventions to improve quality of life of the homeless population.
- Microbial DNA extraction methods for microbial screening - saliva vs biofilm comparisonPublication . Gomes, Ana T. P. C.; Pinto, Marla; Abrantes, Patrícia; Almeida, Rita; Mendes, Karina; Duarte, Ana S.; Silva, Raquel M.; Rosa, Nuno; Correia, Maria; Barros, Marlene
- Molecular & immunological approaches in oral inflammatory diseases: a bridge to precision medicinePublication . Mendes, Karina; Gomes, Ana T. P. C.; Correia, Maria; Pinto, Marla; Marques, Tiago; Rosa, Nuno
- Multiplex immunoassay for inflammatory proteins quantification in saliva – a methodologic approachPublication . Rosa, Nuno; Gomes, Ana T. P. C.; Mendes, Karina; Duarte, Ana S.; Silva, Raquel M. S.; Correia, Maria; Barros, Marlene
- O4 - Exploring the biological properties and regenerative potential of biomaterials using cell culture modelsPublication . Duarte, Ana Sofia; Correia, Bruna L.; Bartolomeu, Maria; Mendes, Karina; Gomes, Ana T. P. C.Life expectancy has improved signifcantly and, along with the declining birthrate, has contributed to the aging of populations, especially in industrialized countries. Alas, aging is intrinsically associated with the incidence of health problems including bone and tooth loss that require suitable solutions to support the quality of life. To meet these demands, signifcant research eforts have been undertaken to develop novel biomaterials, both orthopedic and dental implants. The feld of biomaterials for bone tissue engineering is increasingly evolving. The most recent generations of biomaterials have increasingly more activity and interaction with the biological environment and stimulate the regeneration of functional tissue. Natural polymers and compounds have been combined with each other to improve workability and are strategically integrated with ceramics or bioactive glasses to reinforce the structure of the fnal system, thus producing composites with a better mechanical performance. Our research group has been focused on the biological characterization of diferent added-value materials and composites, namely by evaluating their antimicrobial, biocompatibility, and regenerative properties. Some of our recent work results allowed us to conclude that marine fungal extracts, as well as sol–gel-derived bioactive glass nanoparticles, have inhibitory efects on the growth of C. albicans and E. faecalis (main pathogens in persistent root canal infections). Additionally, we have characterized cuttlefsh bone powders for endodontic applications. We are also committed to developing strategies for monitoring cell response to these biomaterials at the molecular level that could be used to follow infammation and osteoconduction.
- Oral health in inflammatory bowel disease: the overlooked impact and the potential role of salivary calprotectinPublication . Rodrigues, Cláudio; Gomes, Ana T. P. C.; Leal, Joana; Pereira, Pedro; Lopes, Pedro C.; Mendes, Karina; Correia, Maria J.; Veiga, Nélio; Rosa, Nuno; Soares, Caroline; Ministro, PaulaBackground: Inflammatory Bowel Disease (IBD), a chronic condition characterized by gastrointestinal inflammation, is influenced by genetic and environmental factors. Emerging evidence suggests a “mouth-gut axis,” with the oral cavity reflecting extra-intestinal manifestations of IBD. This study evaluated the oral health status of IBD patients and the potential of salivary calprotectin (SCP) as a biomarker for assessing IBD activity and oral health. Methods: Oral health was assessed in 100 IBD patients [60 with Crohn’s disease (CD) and 40 with ulcerative colitis (UC)] and 14 controls. Evaluations included the Decayed, Missing, and Filled Teeth (DMFT) Score, Periodontal Diagnosis and the need for dental or prosthetic treatment. Saliva and stool samples were collected to measure SCP and faecal calprotectin (FCP) levels using the Elia Calprotectin 2 Test. IBD activity was evaluated with FCP, the Harvey-Bradshaw Index for CD, and the Partial Mayo Score for UC. Results: The DMFT index mean was comparable between IBD patients (mean 7.99, SD 7.73) and controls (mean 10.00, SD 6.49). However, periodontal disease was significantly more prevalent in IBD patients (57% in CD, 70% in UC) than in controls (29%), with severe cases (stages III/IV) more frequent in IBD. Additionally, 89% of IBD patients required dental treatment, and 39% needed prosthetic rehabilitation. SCP levels showed no significant correlation with disease activity or oral health status, while FCP correlated with C-reactive protein and erythrocyte sedimentation rate. Conclusions: This study underscores the need for improved oral health management in IBD patients and suggests that SCP may not be a reliable biomarker for monitoring IBD or periodontal disease.
- P9 - Antimicrobial properties and bioactivity potential of smart nanoparticles for dental applicationsPublication . Correia, Bruna L.; Malekshiri, Moslem; Bartolomeu, Maria; Silva, Virgília; Oliveira, Ana; Noites, Rita; Cardoso, Miguel; Mendes, Karina; Gomes, Ana T. P. C.; Duarte, Ana SofiaTooth decay is one of the greatest causes of tooth loss in the world. This not only afects the patient’s quality of life but also carries an economic burden associated with the need for multiple reinterventions. Endodontic treatment aims to preserve teeth by cleaning, disinfecting and flling/sealing the root canal. Despite the high success rate of endodontic treatment, failures do occur in a large number of cases. Several new biomaterials for dentistry have been developed, however their bioactivity is often misunderstood. Our work focuses on the biological characterization of novel bioactive glass nanoparticles, including the evaluation of their antimicrobial and biocompatibility properties. Candida albicans (ATCC 11225) and Enterococcus faecalis (ATCC 29212) were included to evaluate the antimicrobial potential by the drop plate method [1]. The cytotoxicity was tested using the MC3T3-E1 cell line, through the resazurin reduction assay. The novel bioactive glass nanoparticles demonstrated antimicrobial activity against C. albicans and E. faecalis, being able to inhibit their growth but also, in some incubation times, decreased the survival of these microorganisms. After 24 h of incubation of MC3T3-E1 osteoblast cells with bioactive glass nanoparticles conditioned medium, around 48% cell viability was achieved. These novel bioactive glass nanoparticles have shown promising properties which may fnd applications on diferent areas of clinical dentistry.