Browsing by Author "Magalhães, Joana"
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- Estratégias para estimulação cognitiva das pessoas idosas com demênciaPublication . Proença, Bárbara; Magalhães, Joana; Pereira, Raquel; Costa, Tânia
- Molecular techniques and target selection for the identification of Candida spp. in oral samplesPublication . Magalhães, Joana; Correia, Maria José; Silva, Raquel M.; Esteves, Ana Cristina; Alves, Artur; Duarte, Ana SofiaCandida species are the causative agent of oral candidiasis, with medical devices being platforms for yeast anchoring and tissue colonization. Identifying the infectious agent involved in candidiasis avoids an empirical prescription of antifungal drugs. The application of high-throughput technologies to the diagnosis of yeast pathogens has clear advantages in sensitivity, accuracy, and speed. Yet, conventional techniques for the identification of Candida isolates are still routine in clinical and research settings. Molecular approaches are the focus of intensive research, but conversion into clinic settings requires overcoming important challenges. Several molecular approaches can accurately identify Candida spp.: Polymerase Chain Reaction, Microarray, High-Resolution Melting Analysis, Multi-Locus Sequence Typing, Restriction Fragment Length Polymorphism, Loop-mediated Isothermal Amplification, Matrix Assisted Laser Desorption Ionization-mass spectrometry, and Next Generation Sequencing. This review examines the advantages and disadvantages of the current molecular methods used for Candida spp. Identification, with a special focus on oral candidiasis. Discussion regarding their application for the diagnosis of oral infections aims to identify the most rapid, affordable, accurate, and easy-to-perform molecular techniques to be used as a point-of-care testing method. Special emphasis is given to the difficulties that health care professionals need to overcome to provide an accurate diagnosis.
- Percepção dos professores de educação regular e educação especial acerca das novas políticas de educação inclusiva em PortugalPublication . Magalhães, Joana; Veríssimo, Lurdes; Veiga, ElisaThis article presents the result of a research project which its main objective was to explore the impact of the new inclusive education policies in the special education teachers’ and regular education teachers’ perceptions and attitudes. So, an exploratory study was conducted and four special education teachers and four regular education teachers were interviewed. The results of the research have highlighted that there is a positive attitude towards the legislation and inclusion, although they are many aspects that need to be improved, such as human and material resources.