Browsing by Author "Fernandes, Mariana"
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- Campylobacter spp. as a foodborne pathogen: a reviewPublication . Silva, Joana; Leite, Daniela; Fernandes, Mariana; Mena, Cristina; Gibbs, Paul Anthony; Teixeira, PaulaCampylobacter is well recognized as the leading cause of bacterial foodborne diarrheal disease worldwide. Symptoms can range from mild to serious infections of the children and the elderly and permanent neurological symptoms. The organism is a cytochrome oxidase positive, microaerophilic, curved Gram-negative rod exhibiting corkscrew motility and is carried in the intestine of many wild and domestic animals, particularly avian species including poultry. Intestinal colonization results in healthy animals as carriers. In contrast with the most recent published reviews that cover specific aspects of Campylobacter/campylobacteriosis, this broad review aims at elucidating and discussing the (i) genus Campylobacter, growth and survival characteristics; (ii) detection, isolation and confirmation of Campylobacter; (iii) campylobacteriosis and presence of virulence factors; and (iv) colonization of poultry and control strategies.
- Marketing accountability and marketing automation: evidence from PortugalPublication . Silva, Susana C.; Corbo, Leonardo; Vlačić, Božidar; Fernandes, MarianaPurpose: The present study seeks to outline the role of marketing automation (MA) in measuring the return on marketing activities and the challenges associated with reaching accountability in marketing. Design/methodology/approach: To investigate the objective of the study, the authors adopted a qualitative approach, conducting an exploratory study among ten key informants located in Portugal. Findings: Based on the results of the qualitative analysis, a conceptual framework is proposed, which includes both strategic- and operational-level factors with the goal of creating a value-based agenda. In this agenda, executives such as the Chief Marketing Officer emerge as value creators, fostering business scalability, and further arguments are provided to justify budget allocation to MA activities. Originality/value: Through careful research of the elements that characterize the phenomenon under study, the present paper ultimately contributes to a better understanding of MA and accountability within the current business paradigm.
- Noite europeia dos investigadores em Armamar: palco da ciência em ambientes ruraisPublication . Branquinho, Raquel; Duarte, Joaquim; Sarabando, Cândida; Damião, Cláudia; Ambrósio, Susana; Barbosa, Joana Cristina; Barbosa, Joana Bastos; Rezende, Lourenço; Carocho, Márcio; Fernandes, Mariana; Marques, Richard; Friães, Sofia; Santos Carvalho, Ana; Teixeira, Bruno; Ribeiro, Daniela; Grosso, Filipa; Duarte, Inês; Teixeira, José Carlos; Saavedra, Maria José; Alvim, Mariana; Lúcio, Marlene; Oliveira, Mónica; Cardoso Santos, Nuno; Cardoso, Paula; Nunes, Paulo; Cruz, Pedro; Bartolomeu, Raul; Cardoso, Ricardo; Ferreira, Sónia; Carvalho, Teresa; Teixeira, Sofia; Fonseca, Joana; Duarte, Hernâni; Sá-Pinto, Xana; Gomes, Joana; Soares, Luís
- Study of cytolethal distending toxin (cdt ) in campylobacter coli using a multiplex polymerase chain reaction assay and its distribution among clinical and food strainsPublication . Fernandes, Mariana; Mena, Cristina; Silva, Joana; Teixeira, PaulaFrom a collection of 242 food isolates of Campylobacter spp. 72 were identified as Campylobacter jejuni and 170 as Campylobacter coli and of 112 isolates from clinical sources, 92 were identified as C. jejuni and 20 as C. coli. The results for food isolates were in contrast to other reported identifications. Twenty C. coli strains from clinical cases of Campylobacter that occurred in Portugal and 170 isolated from poultry products were examined for the presence of cytolethal distending toxin (cdt) by multiplex polymerase chain reaction assay. CdtA, cdtB, and cdtC were present in 100% of the clinical strains. With respect to the food isolates, the cdtA and cdtC genes were detected in 99.4% of the strains and cdtB in 98.8%. Small point mutations or major deletions within the primer binding sites are possible explanations for these observations but need to be further investigated.