Browsing by Author "Feio, Gabriel"
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- Fresh-cut melon quality during storage: an NMR study of water transverse relaxation timePublication . Fundo, Joana F.; Amaro, Ana L.; Madureira, Ana Raquel; Carvalho, Alexandra; Feio, Gabriel; Silva, Cristina L. M.; Quintas, MafaldaMolecular mobility is a fundamental parameter which reflects the dynamic properties of food components and contributes to food degradation reactions comprehension. Fresh-cut fruits have become an important food market segment. However, processing of fruits promotes faster its physiological deterioration, biochemical changes and microbial degradation. The purpose of this work was to use NMR methodology as a tool to evaluate fresh-cut fruit quality, during storage at refrigerated conditions. The fresh-cut melon transverse relaxation time (T2) was measured for a period of 7 days of storage at 5 °C. The relationship between the obtained values, microstructure and quality parameters was investigated. In general, results show the existence of one class of water fluidity in the system, the one present in cells after processing. T2, a measure of this fluidity, is affected by the processing and storage time. Also, it is possible to find a close relationships between T2 and quality parameters of total colour difference (TCD), firmness and aw. As T2 increases TCD also increases, while firmness and aw decrease. These results highlight the usefulness of NMR methodology application in food science.
- Fresh-cut pear quality during storage: a NMR study of water transverse relaxation timePublication . Fundo, Joana; Carvalho, Alexandra; Feio, Gabriel; Silva, Cristina L.M.; Quintas, Mafalda A. C.Fresh-cut fruits have become an important food market segment due to increasing demand for fresh, healthy and convenient foods. However, processing fruits promotes a decrease in its stability with a faster physiological deterioration, biochemical changes and microbial degradation. Recently, food stability is strongly attributed to molecular dynamics and “water availability”. Understanding cooperatively changes in location and mobility of water is particularly important, considering that water dynamics profoundly influences physicochemical and microbiological quality of foods. The aim of this study was to use nuclear magnetic resonance spectroscopy (NMR) as a tool to evaluate storage fresh-cut fruit quality. Recently, NMR has evolved to become a powerful methodology to probe the molecular dynamics of food constituents, which in turns is a fundamental parameter to determine the dynamic properties of food components and contributes to food degradation reactions comprehension. In this work fresh-cut pear transverse relaxation time (T2) was measured for a period of 7 days of storage at 5 °C. The relationship between the obtained values, microstructure and quality parameters was investigated. In general, results show the existence of one class of water fluidity in the system, the one present in cells after processing. T2, a measure of this fluidity, is affected by the processing and storage time. Also, it is possible to find a relationships between T2 and the quality parameters: total colour difference (TCD), firmness and aw. T2 increases with aw, while it decreases with TCD and firmness. These results highlight the usefulness of NMR methodology application in food science
- Fresh-cut pear quality during storage; a NMR study of water transverse relaxation timePublication . Fundo, Joana; Carvalho, Alexandra; Feio, Gabriel; Silva, Cristina L. M.; Quintas, Mafalda
- Molecular mobility, composition and structure analysis in glycerol plasticised chitosan filmsPublication . Fundo, Joana; Fernandes, Rui; Almeida, Pedro M.; Carvalho, Alexandra; Feio, Gabriel; Silva, Cristina L. M.; Quintas, MafaldaThis study was developed with the purpose to investigate the effect of polysaccharide/plasticiser concentration on the microstructure and molecular dynamics of polymeric film systems, using transmission electron microscope imaging (TEM) and nuclear magnetic resonance (NMR) techniques. Experiments were carried out in chitosan/glycerol films prepared with solutions of different composition. The films obtained after drying and equilibration were characterised in terms of composition, thickness and water activity. Results show that glycerol quantities used in film forming solutions were responsible for films composition; while polymer/total plasticiser ratio in the solution determined the thickness (and thus structure) of the films. These results were confirmed by TEM. NMR allowed understanding the films molecular rearrangement. Two different behaviours for the two components analysed, water and glycerol were observed: the first is predominantly moving free in the matrix, while glycerol is mainly bounded to the chitosan chain.
- A NMR contribute to understand fresh-cut melon behaviour during storagePublication . Fundo, Joana; Carvalho, Alexandra; Feio, Gabriel; Silva, Cristina L. M.; Quintas, Mafalda
- NMR water transverse relaxation time approach to understand storage stability of fresh-cut ‘Rocha’ pearPublication . Fundo, Joana; Galvis-Sanchez, Andrea; Madureira, Ana Raquel; Carvalho, Alexandra; Feio, Gabriel; Silva, Cristina L. M.; Quintas, MafaldaFresh-cut fruit is an important segment in fruit market due to the increasing demand for healthy/ convenient foods. However, processing promotes a decrease in fruits stability with faster physiological and microbiological deteriorations. Food stability is strongly attributed to its molecular dynamics and “water availability”. Understanding changes in water location/mobility is of utmost importance, since water dynamics profoundly influences physic-chemical and microbiological quality of foods. Nuclear magnetic resonance spectroscopy (NMR) is a methodology used to study the food constituents' molecular dynamics. The aim of this study is to use NMR to evaluate changes in water mobility that occurred in fresh-cut pear tissues during storage, by measuring the transverse relaxation time (T2) parameter. Results showed the existence of three water classes in the cells after processing, with T2 values of 10 ms, 187 ms and 3s for cellular wall, cytoplasm and vacuole, respectively. Also, the obtained results demonstrated that T2 was affected by processing and storage. Moreover, a relationship between T2, microstructure and the quality parameters was established. T2, maximum value increased with pear hardness as well as water activity. On the contrary, a decrease in total colour difference (TCD) was found with T2. Results demonstrate the usefulness of the application of NMR relaxometry in food studies.
- Relationship between molecular mobility, microstructure and functional properties in chitosan/glycerol filmsPublication . Fundo, Joana; Carvalho, Alexandra; Feio, Gabriel; Silva, Cristina L. M.; Quintas, Mafalda A.C.Foods are partially crystalline partially amorphous systems. Edible films are considered good models for food systems due to their interesting physical properties, quite straightforward matrices, and easy reproduction. Chitosan is a semicrystalline biopolymer, biocompatible, biodegradable, with antimicrobial activity and filmogenic properties, thoroughly used in edible films' studies. This work aims to investigate the relationship between chitosan films' molecular relaxation time, their microstructure (crystallinity) and functional properties. Analyses were carried out using data on chitosan/glycerol films prepared with different polymer/plasticisant concentrations. Results demonstrate that there is a relationship between macroscopic properties and water and glycerol relaxation times. Moreover, results show that while water is free in the matrix, glycerol is linked to the chitosan polymeric chains, decreasing intermolecular attractions and increasing free volume, thus facilitating molecular migration. Also the data analysis reveals the usefulness of NMR and molecular mobility studies in the matrix for characterisation and development of polymeric structures. Industrial relevance: NMR spectroscopy is currently one of the key methods for food characterisation. Foodstuff is a complex matrix including many different compounds with different chemical structures, concentrations, solubility, properties and nutritional values. From a fundamental perspective, foods are mainly edible and digestible biopolymers that are partially crystalline/partially amorphous and thus edible films, specifically chitosan/glycerol films can be very interesting food model systems for mobility and microstructure studies. Studies on water and solids' mobility and thermo-mechanical properties in food systems (real or model systems),are fundamental to fully attain food physical properties and stability. These studies may be extremely useful for food product and process design, safety and sensorial attributes and also for better understanding and predicting, for example, food storage stability conditions.