Browsing by Author "Correia, Maria J."
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- An ecosystem within our mouthPublication . Correia, Maria J.; Rosa, Nuno; Arrais, Joel; Oliveira, José Luis; Barros, Marlene
- Antibacterial effect of silver diamine fluoride incorporated in fissure sealantsPublication . Veiga, Nélio; Ferreira, Paula; Correia, Tiago; Correia, Maria J.; Pereira, Carlos; Amaral, Odete; Correia, Ilídio J.Introduction: The application of fissure sealants is considered to be an important primary prevention method used in dental medicine. However, one of the most common reasons of dental caries development in teeth with fissure sealants is due to the formation of microleakages. The association between various dental biomaterials may limit the major disadvantages and limitations of biomaterials functioning in a complementary manner. The present study consists in the incorporation of a cariostatic agent – silver diamine fluoride (SDF) – in a resin-based fissure sealant followed by the study of release kinetics by spectrophotometry analysis of the association between both biomaterials and assessment of the inhibitory effect on the growth of the reference bacterial strain Streptococcus mutans (S. mutans) in an in vitro study. Materials and Methods: An experimental in vitro study was designed consisting in the entrapment of SDF (Cariestop® 12% and 30%) into a commercially available fissure sealant (Fissurit® ), by photopolymerization and photocrosslinking. The same sealant, without SDF was used as a negative control. The effect of the sealants on the growth of S. mutans was determined by the presence of bacterial inhibitory halos in the cultures at the end of the incubation period. In order to confirm the absence of bacteria in the surface of the materials, Scanning Electron Microscopy (SEM) characterization was performed. Also, to analyze the release profile of SDF along time, spectrophotometry technique was applied. Results: The obtained results indicate that the association of SDF to a resin-based fissure sealant may be able to increase the inhibition of S. mutans growth. However, no SDF release was noticed during the in vitro release studies and no statistical significant difference was verified when comparing the inhibitory halo sizes obtained for test and control group. Conclusions: In this study, the entrapment of SDF in the resin-based fissure sealant did not potentiate the antibacterial effect of the fissure sealant or avoid the immediate development of dental caries. The development of more laboratorial research and, afterwards, long-term clinical data are necessary in order to verify if this association between these biomaterials is effective and can be considered for being used in oral health management. Also, other methodologies for associating cariostatic agents and sealant should be addressed.
- Computational prediction of the human-microbial oral interactomePublication . Coelho, Edgar D.; Arrais, Joel P.; Matos, Sérgio; Pereira, Carlos; Rosa, Nuno; Correia, Maria J.; Barros, Marlene; Oliveira, José L.Background: The oral cavity is a complex ecosystem where human chemical compounds coexist with a particular microbiota. However, shifts in the normal composition of this microbiota may result in the onset of oral ailments, such as periodontitis and dental caries. In addition, it is known that the microbial colonization of the oral cavity is mediated by protein-protein interactions (PPIs) between the host and microorganisms. Nevertheless, this kind of PPIs is still largely undisclosed. To elucidate these interactions, we have created a computational prediction method that allows us to obtain a first model of the Human-Microbial oral interactome.Results: We collected high-quality experimental PPIs from five major human databases. The obtained PPIs were used to create our positive dataset and, indirectly, our negative dataset. The positive and negative datasets were merged and used for training and validation of a naïve Bayes classifier. For the final prediction model, we used an ensemble methodology combining five distinct PPI prediction techniques, namely: literature mining, primary protein sequences, orthologous profiles, biological process similarity, and domain interactions. Performance evaluation of our method revealed an area under the ROC-curve (AUC) value greater than 0.926, supporting our primary hypothesis, as no single set of features reached an AUC greater than 0.877. After subjecting our dataset to the prediction model, the classified result was filtered for very high confidence PPIs (probability ≥ 1-10-7), leading to a set of 46,579 PPIs to be further explored.Conclusions: We believe this dataset holds not only important pathways involved in the onset of infectious oral diseases, but also potential drug-targets and biomarkers. The dataset used for training and validation, the predictions obtained and the network final network are available at http://bioinformatics.ua.pt/software/oralint.
- Do clear aligners release toxic chemicals? - a systematic reviewPublication . Ferreira, Mariana; Costa, Hélder; Veiga, Nélio; Correia, Maria J.; Gomes, Ana T. P. C.; Lopes, Pedro C.The use of advanced polymer materials in clear aligners, such as polyurethane and PET-G, has gained widespread acceptance due to their mechanical properties, biocompatibility and aesthetic appeal. However, concerns persist regarding their potential to release chemical compounds. Our goal is to understand the impact of the toxicity of clear aligners on oral cavity of orthodontic patients. An extensive systematic search was conducted in electronic databases PubMed, Scopus and Cochrane, to identify articles with relevant data. This systematic review will be conducted following the Preferred Reporting Items for Systematic reviews and Meta-analysis guidelines to answer a question formulated according to the Population, Intervention, Com-parison and Outcomes strategy. Four hundred and thirteen articles potentially relevant were identified and after applying PRISMA guidelines 7 articles were included in this review. Our results suggest that clear aligners are generally safe, concerns remain regarding the chemical leaching of thermoplastic materials, bacterial accumulation due to reduced saliva flow, and mild inflammatory responses. Our review empha-sizes that although most materials are biocompatible, some exhibit moderate cytotoxicity, underscoring the need for continued research and improvements in material composition.
- Do clear aligners release toxic chemicals? - a systematic reviewPublication . Ferreira, Mariana; Costa, Hélder; Veiga, Nélio; Correia, Maria J.; Gomes, Ana T. P. C.; Lopes, Pedro C.Clear aligners are a modern orthodontic solution designed to address dental malocclusions discreetly and effectively. In terms of clinical side effects, the most reported issues among aligner users are mild irritation of the oral mucosa, localized inflammation, and hypersensitivity. The use of advanced polymer materials in clear aligners, such as polyurethane and PET-G, has gained widespread acceptance due to their mechanical properties, biocompatibility, and aesthetic appeal. However, concerns persist regarding their potential to release chemical compounds. Our goal is to understand the impact of clear aligner toxicity on the oral cavity of orthodontic patients. An extensive systematic search was conducted in the electronic databases PubMed, Scopus, and Cochrane to identify articles with relevant data. This systematic review was conducted following the Preferred Reporting Items for Systematic reviews and Meta-analysis guidelines (PRISMA) to answer a question formulated according to the Population, Intervention, Comparison, and Outcomes. Four hundred and thirteen articles potentially relevant were identified and after applying PRISMA guidelines and inclusion/exclusion criteria, seven articles were included in this review. Our results suggest that clear aligners are generally safe, but concerns remain regarding the chemical leaching of thermoplastic materials, bacterial accumulation due to reduced saliva flow, and mild inflammatory responses. Our review emphasizes that although most materials are biocompatible, some exhibit moderate cytotoxicity, with the potential to impact these patients’ oral health, underscoring the need for continued research and improvements in material composition.
- Oral health in inflammatory bowel disease: the overlooked impact and the potential role of salivary calprotectinPublication . Rodrigues, Cláudio; Gomes, Ana T. P. C.; Leal, Joana; Pereira, Pedro; Lopes, Pedro C.; Mendes, Karina; Correia, Maria J.; Veiga, Nélio; Rosa, Nuno; Soares, Caroline; Ministro, PaulaBackground: Inflammatory Bowel Disease (IBD), a chronic condition characterized by gastrointestinal inflammation, is influenced by genetic and environmental factors. Emerging evidence suggests a “mouth-gut axis,” with the oral cavity reflecting extra-intestinal manifestations of IBD. This study evaluated the oral health status of IBD patients and the potential of salivary calprotectin (SCP) as a biomarker for assessing IBD activity and oral health. Methods: Oral health was assessed in 100 IBD patients [60 with Crohn’s disease (CD) and 40 with ulcerative colitis (UC)] and 14 controls. Evaluations included the Decayed, Missing, and Filled Teeth (DMFT) Score, Periodontal Diagnosis and the need for dental or prosthetic treatment. Saliva and stool samples were collected to measure SCP and faecal calprotectin (FCP) levels using the Elia Calprotectin 2 Test. IBD activity was evaluated with FCP, the Harvey-Bradshaw Index for CD, and the Partial Mayo Score for UC. Results: The DMFT index mean was comparable between IBD patients (mean 7.99, SD 7.73) and controls (mean 10.00, SD 6.49). However, periodontal disease was significantly more prevalent in IBD patients (57% in CD, 70% in UC) than in controls (29%), with severe cases (stages III/IV) more frequent in IBD. Additionally, 89% of IBD patients required dental treatment, and 39% needed prosthetic rehabilitation. SCP levels showed no significant correlation with disease activity or oral health status, while FCP correlated with C-reactive protein and erythrocyte sedimentation rate. Conclusions: This study underscores the need for improved oral health management in IBD patients and suggests that SCP may not be a reliable biomarker for monitoring IBD or periodontal disease.
- Root-to-shoot ABA signaling does not contribute to genotypic variation in stomatal functioning induced by high relative air humidityPublication . Carvalho, Dália R. A.; Fanourakis, Dimitrios; Correia, Maria J.; Monteiro, José A.; Araújo-Alves, José Pedro L.; Vasconcelos, Marta W.; Almeida, Domingos P. F.; Heuvelink, Ep; Carvalho, Susana M. P.High relative air humidity (RH 85%) during leaf expansion hampers stomatal responsiveness to closing stimuli, a genotype-dependent effect. Genotypes with reduced stomatal closure in response to closing stimuli (i.e., sensitive genotypes) show low bulk leaf abscisic acid concentration ([ABA]). We hypothesized that the amount of ABA produced in the roots and delivered with the transpiration stream to the leaves is a critical step for a proper stomatal functioning in high RH-grown plants. Ten genotypes from a cut rose tetraploid population were grown at moderate (62%) or high (89%) RH. Stomatal anatomy and responsiveness to desiccation or ABA feeding were evaluated. Root and leaf petiole xylem sap [ABA] were quantified, and ABA delivery rate from root-to-shoot was estimated. High RH reduced stomatal responsiveness to both closing stimuli in the sensitive genotypes, whereas it had a nonsignificant effect in the tolerant ones. Estimates of [ABA] in root xylem sap at transpirational flow rate were not related to the genotypic differences in the sensitivity to high RH. However, high RH increased this concentration irrespective of the genotype, probably due to a reduced dilution of the [ABA] in the xylem sap as a result of a lower transpiration rate compared to moderate RH-grown plants. Leaf petiole xylem sap [ABA] was neither related to the genotypic differences in the sensitivity to high RH nor it was affected by RH. We concluded that genotypic differences in the stomatal sensitivity to high RH cannot be explained by changes in the [ABA] delivered with the transpiration stream from the roots to the leaves
- Unlocking the potential of probiotic administration in caries management: a systematic reviewPublication . Lopes, Pedro C.; Gomes, Ana T. P. C.; Mendes, Karina; Blanco, Letícia; Correia, Maria J.Background The use of prebiotics and/or probiotic bacteria with the potential to modulate the oral ecosystem may play an important role in the prevention and management of dental caries. To assess the evidence of the potential of pre/probiotics both in the prevention and treatment of dental caries, we focused on the PICO question “In individuals with caries, after probiotic administration, is there an improvement in outcomes directly related to caries risk and development?“. Methods An extensive systematic search was conducted in electronic databases PubMed, Web of Science, Scopus and Cochrane, to identify articles with relevant data. This systematic review included trials performed in Humans; published in English; including the observation of patients with caries, with clear indication of the probiotic used and measuring the outcomes directly involved with the cariogenic process, including the quantification of bacteria with cariogenic potential. To evaluate the methodological quality of the studies, the critical assessment tool from the Joanna Briggs Institute was used. Results Eight hundred and fifty articles, potentially relevant, were identified. Following PRISMA guidelines 14 articles were included in this systematic review. Outcomes such as reduction of cariogenic microorganism counts, salivary pH, buffer capacity, and caries activity were assessed. The probiotic most often referred with beneficial results in dental caries outcomes is Lacticaseibacillus rhamnosus. Regarding the most used administration vehicle, in studies with positive effects on the caries management, probiotic supplemented milk could be considered the best administration vehicle. Conclusions Evidence suggests a beneficial effect of probiotic supplemented milk (Lacticaseibacillus rhamnosus) as an adjuvant for caries prevention and management. However, comparable evidence is scarce and better designed and comparable studies are needed.
- Unravelling the benefits of thermal waters enhancing oral health: a pilot studyPublication . Lopes, Sara; Morgado, Susana; Gomes, Ana T. P. C.; Lopes, Pedro C.; Couto, Patrícia; Correia, Maria J.; Flores-Fraile, Javier; Veiga, NélioBackground: Oral health represents a public health problem due to its remarkable social impact and medical costs. Crenotherapy with sulfur water is shown to be a complementary, less toxic, and traumatizing therapy, but the number of studies that evaluate the effect of natural mineral waters effect on oral health are scarce. The aim of this pilot study is to evaluate the impact of thermal water therapy on the oral health of the participants, assessing parameters such as plaque index, gingival bleeding index and periodontal probing depth as well on the perception of symptoms of oral mucosa diseases (OMD). Methods: An observational, longitudinal and comparative study was designed, and 90 thermalists were randomly allocated to two treatment groups for 14 days: Thermal sulfuric natural mineral water of the Amarante Thermal baths group (TW_TA group) (n = 45) or saline solution (control group) (n = 45), in May 2022. The study was based on clinical observation and application of a self-response questionnaire involving sociodemographic data and quality of life assessment. The evaluation was carried out in 2 different moments: before and at the end of treatment (14 days). Results: The study involved 90 thermal practitioners, evenly split between the TW_TA group and a control group. Most participants were women (70%), with a similar average age in both groups. Oral examination showed a high prevalence of filled and missing teeth, and around 25% of participants used removable prostheses, predominantly in the control group. Thermal treatment had a positive impact on oral health. In the TW_TA group, gingival bleeding significantly decreased from 68.9% to 40%, while it remained unchanged in the control group. Periodontal health improved, with no participants in the TW_TA group having pockets deeper than 5 mm by the end of the study, indicating reduced periodontal pathology. Also, plaque levels dropped in both groups after treatment, as assessed by the O’Leary index. Additionally, quality of life related to OMD improved, particularly in the TW_TA group. The overall reduction in symptoms was significant, although the differences between groups were not statistically significant. Conclusions: This study demonstrates the positive effects of thermal water treatment on oral health, including reduced gingival bleeding and plaque levels, along with improved quality of life related to OMD. Further targeted research is needed to explore the benefits of thermal water effects and optimize oral health practices in Portugal using thermal waters.
- White spot lesion treatment options: a systematic review of different techniques for masking these lesionsPublication . Lamorgese, Michela; Veiga, Nélio; Correia, Maria J.; Gomes, Ana T. P. C.; Lopes, Sara; Lopes-Rocha, Lígia; Fidalgo-Pereira, Rita; Lopes, Pedro C.White spot lesions (WSLs) are early clinical stages of enamel demineralization, often related to orthodontic treatment or poor oral hygiene. The use of gels such as fluoride for topical application inhibits demineralization and promotes remineralization of dental tissues through various mechanisms. A variety of therapeutic approaches are available; however, recent research indicates that combined treatment strategies may yield superior clinical outcomes compared to monotherapy. The aim of this study was to critically compare the efficacy of combining multiple treatment techniques for WSLs compared to using these techniques alone. A systematic search was conducted in PubMed, Scopus, and Cochrane databases according to PRISMA guidelines. The PICO strategy was used to formulate the research question: Which clinical approaches combined or isolated (C) influence the treatment and prevention effectiveness (O) of white spot lesions (I) in humans (P) in the last ten years (T)? Inclusion criteria focused on clinical studies from the last ten years evaluating the combined use of at least two treatment techniques for WSL, resulting in a total of 8 randomized controlled trials selected from an initial pool of 1185 articles. Our results suggest that combined treatment strategies, including resin infiltration with fluoride varnish and ozone therapy combined with fluoride application, demonstrated enhanced efficacy in lesion masking and remineralization compared to single-treatment approaches. CPP-ACP and hydroxyapatite-based creams improved aesthetics, particularly when used alongside fluoride varnish. Our study concluded that the combination of remineralization agents like fluoride gel, infiltrative resins, and antimicrobial treatments offers superior outcomes on white spot lesion treatment than using these techniques alone. However, long-term clinical studies are needed to standardize treatment protocols and confirm durability.