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Percorrer por autor "Carocho, Marcio"

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  • Plant extracts and SARS-CoV-2: research and applications
    Publication . Heleno, Sandrina A.; Carocho, Marcio; Reis, Filipa S.; Pires, Tânia C. S. P.; Pintado, Manuela; Ferreira, Isabel C. F. R.; Barros, Lillian
    The recent pandemic of COVID-19 caused by the SARS-CoV-2 virus has brought upon the world an unprecedented challenge. During its acute dissemination, a rush for vaccines started, making the scientific community come together and contribute to the development of efficient therapeutic agents and vaccines. Natural products have been used as sources of individual molecules and extracts capable of inhibiting/neutralizing several microorganisms, including viruses. Natural extracts have shown effective results against the coronavirus family, when first tested in the outbreak of SARS-CoV-1, back in 2002. In this review, the relationship between natural extracts and SARS-CoV is discussed, while also providing insight into misinformation regarding the use of plants as possible therapeutic agents. Studies with plant extracts on coronaviruses are presented, as well as the main inhibition assays and trends for the future regarding the yet unknown long-lasting effects post-infection with SARS-CoV-2.
    2023-01-31Artigo científico Acesso aberto Ver mais
  • Response surface methodology applied to essential oil extraction of eucalyptus leaves
    Publication . Hached, Hanine; Pedrosa, Mariana C.; Heleno, Sandrina; Barros, Lillian; Vaz, Josiana; Carocho, Marcio
    Plant volatiles are secondary metabolites with a wide range of applications in several industries, namely the textile, food, pharma, and perfumery. These molecules are usually from three major groups, the terpenoid, phenylpropanoid/benzenoid and fatty acid derivatives group. This work focused on applying the response surface methodology technique towards optimizing the yield in essential oils of Eucalyptus globulus Labill, extracted by hydro distillation. For this, three factors were varied, namely the time of extraction (variation between 180 to 270 min), particle size (varying between 1 and 3 mm), and solid/liquid ration (varying between 10 and 50 g/L). The response recorded was the amount (g) of recovered essential oil. Using the optimization function, the optimal points were set at 260 minutes of extraction time, particle size of 1.2 mm and 50 g/L ratio. The model was significant, and the lack of fit was not, allowing for an R2 of 0.9911 and an adjusted R2 of 0.9777. Figure 1 shows the 3D charts of the response-surface. The model predicted that, at the optimal point, the yield in essential oils is 0.707 g, an amount above the one found in any of the 17 extraction conditions performed. Overall, the most important of the three factors was the solid to liquid ratio, showing that this factor accounts for most variations in the amount of pure essential oil, followed by particle size and finally, the least important factor, extraction time. This study allows an optimization of the extraction of essential oils from eucalyptus by showing that larger amounts of extract render more oil, as well as smaller particle sizes, while the extraction time has a low influence, thus allowing for shorter extraction times, which corresponds to lower energetic waste. The results herein are important for the food industry, specifically for new food additives, but also as flavorings for grain-based foods.
    2022-03-23Atas de conferência Acesso aberto Ver mais