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|Título:||Evaluation of some carotenoids in grapes by reversed-and normal-phase liquid chromatography : A qualitative analysis|
|Autor:||Manuela Mendes-Pinto, Maria|
Ferreira, A. C. Silva
Oliveira, M. Beatriz P. P.
Pinho, Paula Guedes de
|Palavras-chave:||Port wine grapes|
|Editora:||American Chemical Society|
|Citação:||"Journal of Agricultural and Food Chemistry" . ISSN 0021-8561. 52:10 (2004) 3182-3188|
|Resumo:||Carotenoids in grapes of three Port winemaking cultivars were investigated. Extracts were obtained with n-hexane/diethyl ether mixtures (0/100; 20/80; 50/50; 100/0) and analyzed by normal and reversed phase HPLC-DAD. Selection and identification of peaks were based on spectroscopic characteristics - ìmax, (%III/II) and k¢ values, leading to 28 probable carotenoids. Using pure standards, it was possible to identify seven compounds previously described (neochrome, neoxanthin, violaxanthin, flavoxanthin, zeaxanthin, lutein, and â-carotene), one more type of neochrome reported here, for the first time, and in addition, two geometrical isomers of lutein and â-carotene were tentatively described. The remaining 17 need to be further identified. High polarity solvent mixtures lead to qualitatively richer chromatograms. Reversed-phase separations allowed the detection of flavoxanthin and the possible geometrical isomer(s) of â-carotene. Under normal phase, zeaxanthin was detected, and neochromes were better separated from neoxanthin. Extraction with 50/50 n-hexane/diethyl ether mixtures and reversed-phase conditions was the best combination for analysis of the carotenoids, known as precursors of compounds with high aroma impact in wines.|
|Versão do Editor:||http://pubs.acs.org/doi/abs/10.1021/jf0499469|
|Aparece nas colecções:||ESB - Artigos em revistas internacionais com Arbitragem / Papers in international journals with Peer-review|
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