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Evaluation of some carotenoids in grapes by reversed-and normal-phase liquid chromatography : A qualitative analysis

2005-04Journal article Restricted access
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dc.contributor.authorManuela Mendes-Pinto, Maria
dc.contributor.authorFerreira, A. C. Silva
dc.contributor.authorOliveira, M. Beatriz P. P.
dc.contributor.authorPinho, Paula Guedes de
dc.date.accessioned2011-10-22T10:17:22Z
dc.date.available2011-10-22T10:17:22Z
dc.date.issued2005-04
dc.description.abstractCarotenoids in grapes of three Port winemaking cultivars were investigated. Extracts were obtained with n-hexane/diethyl ether mixtures (0/100; 20/80; 50/50; 100/0) and analyzed by normal and reversed phase HPLC-DAD. Selection and identification of peaks were based on spectroscopic characteristics - ìmax, (%III/II) and k¢ values, leading to 28 probable carotenoids. Using pure standards, it was possible to identify seven compounds previously described (neochrome, neoxanthin, violaxanthin, flavoxanthin, zeaxanthin, lutein, and â-carotene), one more type of neochrome reported here, for the first time, and in addition, two geometrical isomers of lutein and â-carotene were tentatively described. The remaining 17 need to be further identified. High polarity solvent mixtures lead to qualitatively richer chromatograms. Reversed-phase separations allowed the detection of flavoxanthin and the possible geometrical isomer(s) of â-carotene. Under normal phase, zeaxanthin was detected, and neochromes were better separated from neoxanthin. Extraction with 50/50 n-hexane/diethyl ether mixtures and reversed-phase conditions was the best combination for analysis of the carotenoids, known as precursors of compounds with high aroma impact in wines.por
dc.identifier.citation"Journal of Agricultural and Food Chemistry" . ISSN 0021-8561. 52:10 (2004) 3182-3188por
dc.identifier.doi10.1021/jf0499469
dc.identifier.urihttp://hdl.handle.net/10400.14/6778
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherAmerican Chemical Societypor
dc.subjectPort wine grapespor
dc.subjectCarotenoid identificationpor
dc.titleEvaluation of some carotenoids in grapes by reversed-and normal-phase liquid chromatography : A qualitative analysispor
dc.typejournal article
dspace.entity.typePublication
person.familyNameSilva Ferreira
person.familyNameGuedes de Pinho
person.givenNameAntonio Cesar
person.givenNamePaula
person.identifier392870
person.identifier.ciencia-id931A-48DA-23A8
person.identifier.ciencia-id2510-1418-EF48
person.identifier.orcid0000-0002-1188-1021
person.identifier.orcid0000-0002-7395-5700
person.identifier.scopus-author-id6504448214
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublication826c11dd-d070-4b67-ac58-c96675e3f3b8
relation.isAuthorOfPublication0bde318f-79f7-4130-8600-e553f3384cc6
relation.isAuthorOfPublication.latestForDiscovery0bde318f-79f7-4130-8600-e553f3384cc6

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