Publication
Evaluation of some carotenoids in grapes by reversed-and normal-phase liquid chromatography : A qualitative analysis
dc.contributor.author | Manuela Mendes-Pinto, Maria | |
dc.contributor.author | Ferreira, A. C. Silva | |
dc.contributor.author | Oliveira, M. Beatriz P. P. | |
dc.contributor.author | Pinho, Paula Guedes de | |
dc.date.accessioned | 2011-10-22T10:17:22Z | |
dc.date.available | 2011-10-22T10:17:22Z | |
dc.date.issued | 2005-04 | |
dc.description.abstract | Carotenoids in grapes of three Port winemaking cultivars were investigated. Extracts were obtained with n-hexane/diethyl ether mixtures (0/100; 20/80; 50/50; 100/0) and analyzed by normal and reversed phase HPLC-DAD. Selection and identification of peaks were based on spectroscopic characteristics - ìmax, (%III/II) and k¢ values, leading to 28 probable carotenoids. Using pure standards, it was possible to identify seven compounds previously described (neochrome, neoxanthin, violaxanthin, flavoxanthin, zeaxanthin, lutein, and â-carotene), one more type of neochrome reported here, for the first time, and in addition, two geometrical isomers of lutein and â-carotene were tentatively described. The remaining 17 need to be further identified. High polarity solvent mixtures lead to qualitatively richer chromatograms. Reversed-phase separations allowed the detection of flavoxanthin and the possible geometrical isomer(s) of â-carotene. Under normal phase, zeaxanthin was detected, and neochromes were better separated from neoxanthin. Extraction with 50/50 n-hexane/diethyl ether mixtures and reversed-phase conditions was the best combination for analysis of the carotenoids, known as precursors of compounds with high aroma impact in wines. | por |
dc.identifier.citation | "Journal of Agricultural and Food Chemistry" . ISSN 0021-8561. 52:10 (2004) 3182-3188 | por |
dc.identifier.doi | 10.1021/jf0499469 | |
dc.identifier.uri | http://hdl.handle.net/10400.14/6778 | |
dc.language.iso | eng | por |
dc.peerreviewed | yes | por |
dc.publisher | American Chemical Society | por |
dc.subject | Port wine grapes | por |
dc.subject | Carotenoid identification | por |
dc.title | Evaluation of some carotenoids in grapes by reversed-and normal-phase liquid chromatography : A qualitative analysis | por |
dc.type | journal article | |
dspace.entity.type | Publication | |
person.familyName | Silva Ferreira | |
person.familyName | Guedes de Pinho | |
person.givenName | Antonio Cesar | |
person.givenName | Paula | |
person.identifier | 392870 | |
person.identifier.ciencia-id | 931A-48DA-23A8 | |
person.identifier.ciencia-id | 2510-1418-EF48 | |
person.identifier.orcid | 0000-0002-1188-1021 | |
person.identifier.orcid | 0000-0002-7395-5700 | |
person.identifier.scopus-author-id | 6504448214 | |
rcaap.rights | restrictedAccess | por |
rcaap.type | article | por |
relation.isAuthorOfPublication | 826c11dd-d070-4b67-ac58-c96675e3f3b8 | |
relation.isAuthorOfPublication | 0bde318f-79f7-4130-8600-e553f3384cc6 | |
relation.isAuthorOfPublication.latestForDiscovery | 0bde318f-79f7-4130-8600-e553f3384cc6 |
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