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Abstract(s)
Strain SC-087BT, isolated from sewage sludge compost during a study of bacterial diversity in
composts, was characterized. The isolate was a Gram-positive, short rod that was motile,
catalase- and oxidase-negative and able to grow at 27–45 6C, pH 5.5–9.7 and in up to 10%
NaCl. The peptidoglycan was of the B2b type, containing the characteristic amino acids ornithine,
homoserine and hydroxyglutamic acid. The muramic acid residues of the peptidoglycan were
partially glycolylated. The major cell-wall sugar was mannose; traces of xylose were also detected.
The predominant fatty acids, comprising more than 70% of the total, were anteiso-C17 : 0 and
anteiso-C15 : 0, the major respiratory quinone was menaquinone-12 (MK-12) and the G+C
content of the genomic DNA was 72 mol%. Based on analysis of the 16S rRNA gene sequence,
the closest phylogenetic neighbours of strain SC-087BT were members of the family
Microbacteriaceae, showing sequence similarity values of around 96% with members of the
species Microbacterium barkeri (96.0 %), Microbacterium gubbeenense (95.6 %) and
Microbacterium indicum (95.7 %). The chemotaxonomic and phenotypic traits analysed
supported the inclusion of this strain within the genus Microbacterium and the proposal of a novel
species. The name Microbacterium luticocti sp. nov. is proposed and the type strain is SC-087BT
(5DSM 19459T5CCUG 54537T).
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Citation
VAZ-MOREIRA, Ivone...[et al] - Microbacterium luticocti sp. nov., isolated from sewage sludge compost. International Journal of Systematic and Evolutionary Microbiology. ISSN 1466-5026. Vol. 5 (2008), p. 1700–1704
Publisher
Society for General Microbiology