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Advisor(s)
Abstract(s)
The present communication describes the
chemical modification of anhydrous butterfat by interesterification
with oleic acid catalyzed by a lipase of Mucor
javanicus. Two reactor configurations were tested, a
batch-stirred tank reactor containing suspended lipase
and a batch-stirred tank reactor in combination with a
hollow-fiber membrane module containing adsorbed lipase.
The goal of this research was to assess the advantage
of using a (hydrophobic) porous support to immobilize
the lipase in attempts to engineer butterfat with
increased levels of unsaturated fatty acid residues (oleic
acid) at the expense of medium-to-long chain saturated
fatty acids (myristic and palmitic acids). Reactions were
carried out at 40°C in the absence of solvent under controlled
water activity, and were monitored by chromatographic
assays for free fatty acids. The results obtained
indicate that the rate of interesterification using the proposed
reactor configuration is enhanced by a factor
above 100 relative to that using suspended lipase, for the
same protein mass basis. Although hydrolysis of butterfat
occurred to some degree, the enzymatic process that
uses the hollow-fiber reactor was technically superior to
the stirred tank system.
Description
Keywords
Batch-stirred tank reactor Interesterification Immobilized enzyme Butterfat Membrane bioreactor Mucor javanicus
Pedagogical Context
Citation
BALCÃO, Victor M.; MALCATA, F. Xavier - On the performance of a hollow-fiber bioreactor for acidolysis catalyzed by immobilized lipase. Biotechnology and Bioengineering. ISSN 0006-3592. Vol. 60, n.º 1 (1998), p. 114-123
Publisher
John Wiley & Sons, Inc.