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On the performance of a hollow-fiber bioreactor for acidolysis catalyzed by immobilized lipase

1998Journal article Open access
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dc.contributor.authorBalcão, Victor M.
dc.contributor.authorMalcata, F. Xavier
dc.date.accessioned2011-10-21T12:09:12Z
dc.date.available2011-10-21T12:09:12Z
dc.date.issued1998
dc.description.abstractThe present communication describes the chemical modification of anhydrous butterfat by interesterification with oleic acid catalyzed by a lipase of Mucor javanicus. Two reactor configurations were tested, a batch-stirred tank reactor containing suspended lipase and a batch-stirred tank reactor in combination with a hollow-fiber membrane module containing adsorbed lipase. The goal of this research was to assess the advantage of using a (hydrophobic) porous support to immobilize the lipase in attempts to engineer butterfat with increased levels of unsaturated fatty acid residues (oleic acid) at the expense of medium-to-long chain saturated fatty acids (myristic and palmitic acids). Reactions were carried out at 40°C in the absence of solvent under controlled water activity, and were monitored by chromatographic assays for free fatty acids. The results obtained indicate that the rate of interesterification using the proposed reactor configuration is enhanced by a factor above 100 relative to that using suspended lipase, for the same protein mass basis. Although hydrolysis of butterfat occurred to some degree, the enzymatic process that uses the hollow-fiber reactor was technically superior to the stirred tank system.por
dc.identifier.citationBALCÃO, Victor M.; MALCATA, F. Xavier - On the performance of a hollow-fiber bioreactor for acidolysis catalyzed by immobilized lipase. Biotechnology and Bioengineering. ISSN 0006-3592. Vol. 60, n.º 1 (1998), p. 114-123por
dc.identifier.urihttp://hdl.handle.net/10400.14/6653
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherJohn Wiley & Sons, Inc.por
dc.relation.publisherversionThe definitive version is available at www3.interscience.wiley.compor
dc.subjectBatch-stirred tank reactorpor
dc.subjectInteresterificationpor
dc.subjectImmobilized enzymepor
dc.subjectButterfatpor
dc.subjectMembrane bioreactorpor
dc.subjectMucor javanicuspor
dc.titleOn the performance of a hollow-fiber bioreactor for acidolysis catalyzed by immobilized lipasepor
dc.typejournal article
dspace.entity.typePublication
person.familyNameBalcão
person.familyNameMalcata
person.givenNameVictor
person.givenNameFrancisco
person.identifier.ciencia-idC314-A18C-931B
person.identifier.ciencia-id1B13-38A5-35F5
person.identifier.orcid0000-0003-0772-2834
person.identifier.orcid0000-0003-3073-1659
person.identifier.ridI-2182-2012
person.identifier.scopus-author-id6603449856
person.identifier.scopus-author-id7102542478
rcaap.rightsopenAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublication9b0860f7-99b1-4ded-a483-6435882272d0
relation.isAuthorOfPublicationa06c00da-0e2f-4434-8ede-4d7b22c0dfe9
relation.isAuthorOfPublication.latestForDiscoverya06c00da-0e2f-4434-8ede-4d7b22c0dfe9

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