Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.14/6090
Título: Inactivation kinetics of horseradish peroxidase in organic solvents of different hydrophobicity at different water contents
Autor: Saraiva, Jorge
Oliveira, Jorge C.
Oliveira, Susana
Hendrickx, Marc
Palavras-chave: Biocatalysis
Enzyme thermal inactivation
Time-temperature integrators
Water activity
Data: 1996
Editora: Wiley Blackwell
Citação: SARAIVA, Jorge [et al] - Inactivation kinetics of horseradish peroxidase in organic solvents of different hydrophobicity at different water contents. International Journal of Food Science and Technology. ISSN 0950-5423. 31:3 (1996) 233-240
Resumo: The thermal stability of horseradish peroxidase suspensions was studied in three organic solvents of different hydrophobicity (dodecane, octane, and 1-octanol) at three different water contents (14.1, 55.3 and 256.2mg water g−1 dry protein). In these conditions, the enzyme is much more stable than in aqueous solutions (inactivation temperatures were in the range of 125–150°C). The enzyme showed a similar stability when in the presence of organic solvents, compared to the enzyme in a solid matrix without organic solvents with the same water content. The inactivation kinetics was well described by assuming the existence of two iso-enzymes, both inactivating according to a First order model. The lowest value for the z-value of both fractions (around 15°C) was obtained at the higher water content studied. The use of solvent and water content variables should be adequate to develop time-temperature integrators to monitor thermal processes at 100–140°C.
Peer review: yes
URI: http://hdl.handle.net/10400.14/6090
Versão do Editor: The definitive version is available at http://onlinelibrary.wiley.com/doi/10.1046/j.1365-2621.1996.00343.x/abstract
Aparece nas colecções:ESB - Artigos em revistas internacionais com Arbitragem / Papers in international journals with Peer-review

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