Publication
Inactivation kinetics of horseradish peroxidase in organic solvents of different hydrophobicity at different water contents
| dc.contributor.author | Saraiva, Jorge | |
| dc.contributor.author | Oliveira, Jorge C. | |
| dc.contributor.author | Oliveira, Susana | |
| dc.contributor.author | Hendrickx, Marc | |
| dc.date.accessioned | 2011-10-14T14:44:00Z | |
| dc.date.available | 2011-10-14T14:44:00Z | |
| dc.date.issued | 1996 | |
| dc.description.abstract | The thermal stability of horseradish peroxidase suspensions was studied in three organic solvents of different hydrophobicity (dodecane, octane, and 1-octanol) at three different water contents (14.1, 55.3 and 256.2mg water g−1 dry protein). In these conditions, the enzyme is much more stable than in aqueous solutions (inactivation temperatures were in the range of 125–150°C). The enzyme showed a similar stability when in the presence of organic solvents, compared to the enzyme in a solid matrix without organic solvents with the same water content. The inactivation kinetics was well described by assuming the existence of two iso-enzymes, both inactivating according to a First order model. The lowest value for the z-value of both fractions (around 15°C) was obtained at the higher water content studied. The use of solvent and water content variables should be adequate to develop time-temperature integrators to monitor thermal processes at 100–140°C. | por |
| dc.identifier.citation | SARAIVA, Jorge [et al] - Inactivation kinetics of horseradish peroxidase in organic solvents of different hydrophobicity at different water contents. International Journal of Food Science and Technology. ISSN 0950-5423. 31:3 (1996) 233-240 | por |
| dc.identifier.doi | 10.1046/j.1365-2621.1996.00343.x | |
| dc.identifier.uri | http://hdl.handle.net/10400.14/6090 | |
| dc.language.iso | eng | por |
| dc.peerreviewed | yes | por |
| dc.publisher | Wiley Blackwell | por |
| dc.subject | Biocatalysis | por |
| dc.subject | Enzyme thermal inactivation | por |
| dc.subject | Time-temperature integrators | por |
| dc.subject | Water activity | por |
| dc.title | Inactivation kinetics of horseradish peroxidase in organic solvents of different hydrophobicity at different water contents | por |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| person.familyName | Oliveira | |
| person.givenName | Jorge | |
| person.identifier.orcid | 0000-0002-0680-6174 | |
| person.identifier.rid | C-2642-2008 | |
| person.identifier.scopus-author-id | 7202452289 | |
| rcaap.rights | restrictedAccess | por |
| rcaap.type | article | por |
| relation.isAuthorOfPublication | 5227bcfa-cb99-407e-a2ef-3f4eb86d4243 | |
| relation.isAuthorOfPublication.latestForDiscovery | 5227bcfa-cb99-407e-a2ef-3f4eb86d4243 |